Hybrid Matrix Grafts to Favor Tissue Regeneration in Rabbit Femur Bone Lesions

Goy, Dante Pascual; Gorosito, E.; Costa, Hermes S.; Mortarino, Pablo; Pedemonte, Noelia Acosta; Toledo, J.; Mansur, Herman S.; Pereira, Marivalda M.; Battaglino, Ricardo A.; Feldman, Sara

doi:10.2174/1874120701206010085

Cited by 5 publications

(1 citation statement)

References 18 publications

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“…In that report, the glass formed exhibited textural parameters containing sub-micron sized particles and large surface area desirable for in vivo biological applications. Previous studies have reported the formation of glass/PVA composites from tetraethylorthosilicate (TEOS) as the SiO 2 precursor [16][17][18][19][20][21][22]. The current work, therefore, reports a paradigm shift from TEOS to bentonite clay as an economic source of silica.…”

Section: Introductionmentioning

confidence: 91%

RETRACTED ARTICLE: Mechanical and Bioactivity Assessment of Wollastonite/PVA Composite Synthesized from Bentonite Clay

2019

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Glass/polymer composites can mimic the natural structure of bone by possessing a fiber-matrix configuration which provides appropriate physical and biological properties. Wollastonite ceramics are known for their promising bioactivity and biocompatibility when applied in bone regeneration. Polyvinyl alcohol (PVA) has various attractive properties including biocompatibility and degradability which may be exploited as a polymer matrix in composites for biomedical applications. Therefore, a cost-effective method of preparing wollastonite/PVA composites is desirable by starting from bentonite clay as a silica source for the glass, instead of traditional alkoxysilanes. The composite prepared was characterized by mechanical testing, scanning electron microscopy, X-ray diffractometry and Fourier-transform infrared spectroscopy to evaluate its compressive strength, morphology, phase composition and bioactivity, respectively. Results obtained revealed for the composite a compressive strength of 0.3 MPa, the ability to induce apatite on its surface when immersed in a simulated body fluid for 7 days and desirable controlled degradation. Hence, this method can be up-scaled for preparation of wollastonite/PVA composite commercially for possible use in bone regeneration.

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Section: Introductionmentioning

confidence: 91%

RETRACTED ARTICLE: Mechanical and Bioactivity Assessment of Wollastonite/PVA Composite Synthesized from Bentonite Clay

2019

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^{Bone Regeneration Mediated by a Bioactive and Biodegradable Extracellular Matrix-Like Hydrogel Based on Elastin-Like Recombinamers}

Coletta

Ibáñez‐Fonseca

Missana

et al. 2017

Tissue Engineering Part A

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The morbidity of bone fractures and defects is steadily increasing due to changes in the age pyramid. As such, novel biomaterials that are able to promote the healing and regeneration of injured bones are needed to overcome the limitations of auto-, allo-, and xenografts, while providing a ready-to-use product that may help to minimize surgical invasiveness and duration. In this regard, recombinant biomaterials, such as elastin-like recombinamers (ELRs), are very promising as their design can be tailored by genetic engineering, thus allowing scalable production and batch-to-batch consistency, among others. Furthermore, they can self-assemble into physically crosslinked hydrogels above a certain transition temperature, in this case body temperature, but are injectable below this temperature, thereby markedly reducing surgical invasiveness. In this study, we have developed two bioactive hydrogel-forming ELRs, one including the osteogenic and osteoinductive bone morphogenetic protein-2 (BMP-2) and the other the Arg-Gly-Asp (RGD) cell adhesion motif. The combination of these two novel ELRs results in a BMP-2-loaded extracellular matrix-like hydrogel. Moreover, elastase-sensitive domains were included in both ELR molecules, thereby conferring biodegradation as a result of enzymatic cleavage and avoiding the need for scaffold removal after bone regeneration. Both ELRs and their combination showed excellent cytocompatibility, and the culture of cells on RGD-containing ELRs resulted in optimal cell adhesion. In addition, hydrogels based on a mixture of both ELRs were implanted in a pilot study involving a femoral bone injury model in New Zealand white rabbits, showing complete regeneration in six out of seven cases, with the other showing partial closure of the defect. Moreover, bone neoformation was confirmed using different techniques, such as radiography, computed tomography, and histology. This hydrogel system therefore displays significant potential in the regeneration of bone defects, promoting self-regeneration by the surrounding tissue with no involvement of stem cells or osteogenic factors other than BMP-2, which is released in a controlled manner by elastase-mediated cleavage from the ELR backbone.

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Desarrollo Y Crecimiento Femoral De Conejas De La Línea New Zealand

Ruggieri¹,

Ramallo²,

Grenón³

et al. 2021

Acta biol. Colomb.

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En este proyecto se investigan los cambios que acontecen en el desarrollo y crecimiento de conejos hembras de la línea New Zealand (CoNZ) en sus huesos femorales. Los animales fueron mantenidas en jaulas individuales desde las 2 semanas de edad, con comida y agua ad libitum y se sacrificaron en tiempos mensualmente consecutivos: 1, 2, 3, 4, 5, y 6 meses. Tras la obtención de las piezas femorales, y a partir de estudios imagenológicos se determinaron los ángulos del cuello femoral (Af), la longitud total (L), la densidad mineral ósea total, del centro óseo y de la metáfisis femoral (DMOt, DMOco y DMOmf respectivamente), analizándose las variaciones intergrupales por el test Wilcoxon, y corrección de Bonferroni. Se realizaron estudios histológicos de los cortes descalcificados de las piezas femorales. Los análisis sobre los Af mostraron un incremento significativo durante el primer mes mientras que L se estabilizó a partir del 4to mes. Los valores de DMOt mostraron un plateau a partir del cuarto mes, si bien las DMOco y DMOmf ya a partir del tercer mes no mostraron incrementos significativos. Histológicamente se observó para el cuarto mes ausencia de las diferentes zonas características del cartílago de crecimiento metafisiario, con presencia únicamente de un pequeño remanente de células condrales. Desde el quinto mes se observa ausencia total de cartílago, con presencia únicamente de tejido osteoide (TO). La interpretación integrada de los resultados nos permite afirmar, que a partir del cuarto mes de desarrollo, el fémur de CoNZ adquiere características compatibles con un periodo de adultez.

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Hybrid Matrix Grafts to Favor Tissue Regeneration in Rabbit Femur Bone Lesions

Cited by 5 publications

References 18 publications

RETRACTED ARTICLE: Mechanical and Bioactivity Assessment of Wollastonite/PVA Composite Synthesized from Bentonite Clay

RETRACTED ARTICLE: Mechanical and Bioactivity Assessment of Wollastonite/PVA Composite Synthesized from Bentonite Clay

^{Bone Regeneration Mediated by a Bioactive and Biodegradable Extracellular Matrix-Like Hydrogel Based on Elastin-Like Recombinamers}

Desarrollo Y Crecimiento Femoral De Conejas De La Línea New Zealand

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