Exploring Strategic Approaches for CvFAP Photodecarboxylation through Violet Light Irradiation

Abstract

In this study, we describe a light-driven photocatalytic decarboxylation of palmitic acid and related fatty acids using Chlorella variabilis fatty acid photodecarboxylase (CvFAP). By utilizing violet light emitting diode (LED) light (50 W; 397 nm), we achieved a remarkable conversion efficiency of 99% within just 4 min, surpassing the previous 79% conversion achieved in 60 min using blue LED light (300 W; 439 nm). Importantly, the use of 50 W violet LED light also resulted in a lower enzyme photoinactivation rate when compared to 300 W blue LED. Comparing the whole-cell biocatalyst with the enzymatic extract, we found that the former demonstrated superior catalytic performance and reduced susceptibility to photoinactivation. Furthermore, whole-cell biocatalyst reuse was demonstrated after five sequential batches. Employing this approach, we successfully synthesized 26 mmol L-1 h-1 of pentadecane, showcasing a promising strategy to improve productivity. These findings represent a significant advancement in CvFAP photodecarboxylation processes compared to the literature, utilizing an alternative light source, with potential implications to the biofuel sector