The Versatility of Two-Dimensional Liquid Chromatography

Cardoso, Carmen L.; Moraes, Marcela Cristina de; Cass, Quézia B.

doi:10.21577/0103-5053.20230118

Cited by 1 publication

(3 citation statements)

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“…The use of two-dimensional liquid chromatography (2D-LC) has become increasingly common, with easy-to-use, affordable, commercial systems available from a variety of manufacturers [1]. Literature reviews frequently update the current state-of-the-art in 2D-LC [2][3][4][5][6][7] and multi-dimensional LC (MD-LC) [1,8]. Fewer of the published reports employ three-dimensional separation methods [9,10], but the number of these reports is increasing.…”

Section: Introductionmentioning

confidence: 99%

“…In this way, we have three separate dimensions of separation operating at all times during an analysis. Because of this, we do not refer to our parallel 2 Ds simply as an array of columns. We have two complete and independent second dimensions operating simultaneously, instead of one second dimension with multiple columns available.…”

Section: Introductionmentioning

confidence: 99%

“…Note that the superscript prefix designating the dimension number used throughout this report follows the recommended nomenclature from Wu et al [12], which is highly recommended reading. We added parenthetical numbers to differentiate between the first and second 2 Ds, 2 D(1) and 2 D(2).…”

Section: Introductionmentioning

confidence: 99%

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Multi-Dimensional Liquid Chromatography of Pulse Triacylglycerols with Triple Parallel Mass Spectrometry

Byrdwell,

Kotapati

2023

Separations

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We analyzed ten pulses (the dried seeds of legumes), i.e., baby lima beans, black beans, black-eyed peas, butter beans, cranberry beans, garbanzo beans, green split peas, lentils, navy beans, and pinto beans, using three-dimensional liquid chromatography (3D-LC) with parallel second dimensions, LC × (LC + LC). We combined non-aqueous reversed-phase (NARP) chromatography as the first dimension separation, 1D, with argentation UHPLC for separation based on degree and location of unsaturation in the first second dimension, 2D(1), and multi-cycle NARP-UHPLC in the second second dimension, 2D(2). Pulses contained 1.9% to 2.7% lipids, except garbanzo beans, which contained 6.2% lipids. High-resolution, accurate-mass (HRAM) orbitrap mass spectrometry (MS) was used to perform lipidomic analysis of the 2D(2) and percent relative quantification, showing that the most abundant average triacylglycerol (TAG) molecular species across all pulses were PLL at 10.67% and PLLn at 10.45%. Common beans (Phaseolus vulgaris) were clustered together using principal component analysis (PCA), showing the highest levels of linolenic acid, C18:3, in molecular species such as PLnLn, LLnLn, and OLLn, with palmitic (P), C16:0, linoleic (L), 18:2, linolenic (Ln), 18:3, and oleic (O), 18:1, FAs. Calibration curves derived from interweaved sets of regioisomer standards allowed the absolute quantification of 1,2- and 1,3-regioisomers for a subset of TAGs.

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