Phenolic Composition of Dipteryx alata Vogel Pulp + Peel and Its Antioxidant and Cytotoxic Properties

Barizão, Érica Oliveira; Boeing, Joana Schuelter; Rotta, Eliza Mariane; Volpato, Hélito; Nakamura, Celso Vataru; Maldaner, Liane; Visentainer, Jesuí Vergílio

doi:10.21577/0103-5053.20210112

Cited by 2 publications

(2 citation statements)

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“…In a study with extracts from the epicarp + mesocarp (residues) of Dipteryx alata by ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), eighteen phenolic compounds were quantified for the first time, predominantly luteolin and trans-cinnamic acid [56]. These results indicate that species of the Dipteryx genus can be explored as a natural source of phenolic compounds with promising properties.…”

Section: Discussionmentioning

confidence: 98%

Phytochemical Analysis and Antioxidant Activity of Ethanolic Extracts from Different Parts of Dipteryx punctata (S. F. Blake) Amshoff

Sousa,

Gomes,

Viana

et al. 2023

Applied Sciences

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The genus Dipteryx, to which the cumaru tree belongs, contains neotropical species native to Central and South American countries. They are used both in the sale of timber and seeds and for the extraction of the active compound coumarin, used as a flavoring agent. This study evaluated the phytochemical profile and antioxidant activity of extracts of leaves, branches, and fruits (residues and seeds) of the species Dipteryx punctata. The plant material for analysis was collected in five seed-producing areas, in Mojuí dos Campos, Pará, Brazil. The extracts were obtained via Soxhlet extractor using 92.8% distilled ethanol as the solvent and operated till exhaustion (8 h). Chromatographic analyses were performed by thin-layer chromatography (TLC) and gas chromatography coupled to mass spectrometry (GC-MS), followed by phytochemical determination of phenolics and flavonoids and analysis of antioxidant activity (TLC and free radical scavenging 2,2-diphenyl-1-picrylhydrazyl (DPPH). The highest extract yields were obtained from D. punctata fruit residues and seeds from all areas, with maximum values of 26.1% and 47.2%, respectively, in Boa Fé (area 3). In the evaluation by TLC, the extracts of leaves, branches, and residues presented the classes of terpenes, condensed and hydrolysable tannins, and flavonoids; coumarin (1,2-benzopyrone) was identified only in residue and seed extracts. The major constituents highlighted in the collection areas were: lupeol in leaves (34.4% in area 5), 4-O-methylmannose in branches and residues (85.5% in area 2 and 90.6% in area 5, respectively), and coumarin in seeds (99.3% in area 3). The best results for the antioxidant action were obtained for extracts from leaves and residues, requiring a concentration of 117.6 µg.mL−1 of the extract from the leaves and 160.4 µg.mL−1 of the extract from the residues to reduce the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical by 50%. This phytochemical study contributes to reducing the scarcity of information on D. punctata. The chemical classes and compounds identified corroborate the antioxidant activity and add value to the species, and the data obtained reinforce the importance of reusing fruit residues, which are chemically rich but discarded in the environment.

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Section: Discussionmentioning

confidence: 98%

Phytochemical Analysis and Antioxidant Activity of Ethanolic Extracts from Different Parts of Dipteryx punctata (S. F. Blake) Amshoff

Sousa,

Gomes,

Viana

et al. 2023

Applied Sciences

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“…No studies were identified quantifying the contents of total phenolics, tannins, flavonoids, and flavonols in extracts of D. alata leaves. However, Barizão et al (2021) analyzed hydroalcoholic extracts of the pulp and peel of D. alata fruit, finding higher values of phenolic compounds (3.15 ± 0.14 and 2.98 ± 0.01 mg GAE/g) for ethanol and methanol solvents, respectively. These values were lower than those presented by the UCE (50.00 ± 2.5 mg GAE/g), which was the lowest result obtained in this study.…”

Section: Content Of Total Phenolic Compounds Tannins Flavonoids and F...mentioning

confidence: 97%

Phytochemical analysis, toxicity and evaluation of antioxidant and antimicrobial activities of leaves of Dipteryx alata Vogel

Scapin,

Sarri,

Augusco

et al. 2024

Braz. J. Biol.

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In this study, our objective was to conduct a comprehensive phytochemical analysis, determine toxicity levels, and assess the antioxidant and antimicrobial properties of extracts derived from the leaves of Dipteryx alata Vogel, a native species of the Brazilian cerrado flora. Three distinct extracts were prepared utilizing assisted ultrasound and the Soxhlet apparatus, namely, Ultrasound Crude Extract (UCE), Soxhlet Crude Extract (SCE), and the Soxhlet Ethanol Extract (SEE). The phytochemical analysis revealed the presence of flavonoids, tannins, phytosterols, and saponins in all extracts. Additionally, alkaloids were specifically identified in the SCE and SEE extracts. In the analysis using LC-DAD, the compounds gallic acid, rutin, quercetin, luteolin and kampefrol were determined in higher concentrations in the SCE, followed by the SEE and UCE, respectively. The GC-MS analysis revealed the presence of campesterol, stigmasterol and β-sitosterol in all extracts, with UCE and SCE showing a higher concentration of β-sitosterol. SCE showed the highest concentration of all identified compounds. In the analysis of antioxidant activity by DPPH• and ABTS•+, SEE showed greater efficiency (IC50 = 2.98 ± 2.92 and 6.57 ± 0.89 μg/mL, respectively). In the toxicity test with Allium cepa, all extracts stimulated root growth at 50 g/mL; UCE and SEE stimulated root growth at 250 g/mL; and SEE inhibited root growth at 750 g/mL. In the Artemia salina toxicity, all extracts were non-toxic. Antibacterial activity was identified in the microorganisms S. aureus and S. mutans; however, the extracts did not show antifungal action against the strain of C. albicans. The extracts of D. alata have therapeutic potential for applicability in dentistry.

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Phenolic Composition of Dipteryx alata Vogel Pulp + Peel and Its Antioxidant and Cytotoxic Properties

Cited by 2 publications

References 0 publications

Phytochemical Analysis and Antioxidant Activity of Ethanolic Extracts from Different Parts of Dipteryx punctata (S. F. Blake) Amshoff

Phytochemical Analysis and Antioxidant Activity of Ethanolic Extracts from Different Parts of Dipteryx punctata (S. F. Blake) Amshoff

Phytochemical analysis, toxicity and evaluation of antioxidant and antimicrobial activities of leaves of Dipteryx alata Vogel

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