Polyphenol Profile and Quantitative Assessment of the Flavonoid Kaempferitrin in Wild and Cultivated Brazilian Amazonian Uncaria guianensis (Rubiaceae)

Paixão, Djavan da; Barboza, Rodolfo S.; Valente, Lígia M. M.; Souza, Matheus O.; Siani, Antônio Carlos; Pereira, Ricardo; Gallo, Blanca; Berrueta, Luis Á.

doi:10.21577/0103-5053.20210064

Cited by 2 publications

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“…contains polyphenols, kaempferitrin, uncarine C, and E and because of its cytotoxic properties, can damage DNA RS321 and RS322. 10,11 Uncaria tomentosa has an antiproliferative effect against adenocarcinoma of the colon (SW620), breast cancer (MCF7) and gastric cells (AGS), 12 and can increase ROS production in HepG2 cells, resulting in a decrease in GSH levels, meaning apoptosis occurs. 13 Uncaria gambir (Hunter) Roxb has cytotoxic activity on T47D7 breast cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity Screening of Various Uncaria Spp Plants on T47d Breast Cancer Cells

2023

TJNPR

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Breast cancer is a disease characterised by uncontrolled cell growth in breast cells. One of the plants known to have anticancer activity is a plant from Uncaria genus. Some of those found in Province "Riau", Indonesia include Uncaria barbata Merr (UB), Uncaria cordata (Lour) Merr (UC), Uncaria guianensis (Aubl.) J.F.Gmel (UG) and Uncaria nervosa Elmer (UN). This study aimes to determine the cytotoxic effect of ethanolic extract from the leaves and stem bark of Uncaria Spp on T47D breast cancer cells. These were macerated using 70% ethanol and evaporated to obtain a viscous extract. The cytotoxic effect was determined using an MTT assay, and the IC50 value was identified after 48 hours of incubation. The results show that the IC50 values of the ethanolic extracts of the leaves of Uncaria barbata Merr , Uncaria cordata (Lour) Merr , Uncaria guianensis (Aubl.) J.F.Gmel, and Uncaria nervosa Elmer were 227.30, 653.13, 334.65, and 64.42 µg/mL, respectively. The corresponding values of the ethanolic stem bark extracts of Uncaria cordata (Lour) Merr and Uncaria nervosa Elmer were 124.45 and 526.02 µg/mL, respectively. The ethanolic extract of Uncaria nervosa leaves had a strong cytotoxic effect on T47D breast cancer cells, which was 64.42 µg/mL.

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Section: Introductionmentioning

confidence: 99%

Cytotoxic Activity Screening of Various Uncaria Spp Plants on T47d Breast Cancer Cells

2023

TJNPR

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Neuroprotective Properties of Kempferol Derivatives from Maesa membranacea against Oxidative Stress-Induced Cell Damage: An Association with Cathepsin D Inhibition and PI3K/Akt Activation

Jantas

Malarz

et al. 2021

IJMS

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As components of the human diet with potential health benefits, flavonols are the subject of numerous studies, confirming their antioxidant, free radical scavenging and anti-inflammatory activity. Taking into consideration the postulated pathogenesis of certain CNS dysfunctions characterized by neuronal degradation, flavonols may prevent the decay of neurons in multiple pathways. Leaves of Maesa membranacea yielded several flavonol glycosides including α-rhamnoisorobin (kaempferol 7-O-α-rhamnoside) and kaempferitrin (kaempferol 3,7-di-O-α-rhamnoside). The latter compound was a major constituent of the investigated plant material. Neuroprotective effects of kaempferitrin and α-rhamnoisorobin were tested in vitro using H2O2-, 6-OHDA- and doxorubicin-induced models of SH-SY5Y cell damage. Both undifferentiated and differentiated neuroblastoma cells were used in the experiments. α-Rhamnoisorobin at a concentration range of 1–10 µM demonstrated cytoprotective effects against H2O2-induced cell damage. The compound (at 1–10 µM) was also effective in attenuating 6-OHDA-induced neurotoxicity. In both H2O2- and 6-OHDA-induced cell damage, kaempferitrin, similar to isoquercitrin, demonstrated neuroprotective activity at the highest of the tested concentrations (50 µM). The tested flavonols were not effective in counteracting doxorubicin-induced cytotoxicity. Their caspase-3- and cathepsin D-inhibitory activities appeared to be structure dependent. Inhibition of the PI3-K/Akt pathway abolished the neuroprotective effect of the investigated flavonols.

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Polyphenol Profile and Quantitative Assessment of the Flavonoid Kaempferitrin in Wild and Cultivated Brazilian Amazonian Uncaria guianensis (Rubiaceae)

Cited by 2 publications

References 0 publications

Cytotoxic Activity Screening of Various Uncaria Spp Plants on T47d Breast Cancer Cells

Cytotoxic Activity Screening of Various Uncaria Spp Plants on T47d Breast Cancer Cells

Neuroprotective Properties of Kempferol Derivatives from Maesa membranacea against Oxidative Stress-Induced Cell Damage: An Association with Cathepsin D Inhibition and PI3K/Akt Activation

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