Astaxanthin Recovery from Shrimp Residue by Solvent Ethanol Extraction Using Choline Chloride:Glycerol Deep Eutectic Solvent as Adjuvant

Abstract

The present study investigated the use of choline chloride:glycerol deep eutectic solvent as an adjuvant for tuning the ethanol extraction of astaxanthin from Litopenaeus vannamei processing residues. The adjuvant concentration did not show a clear trend on the total carotenoid, however, the addition of adjuvant proved to be advantageous in the ultrasound-assisted experiments. In order to establish a balance between total carotenoid and productivity, an operational condition with ultrasound, 5% (m/v) choline chloride:glycerol and 10 min of incubation was selected, which provided 737.69 μg g-1 total carotenoids and 32.71 μg g-1 astaxanthin. When compared to the astaxanthin standard, the shrimp residue extract obtained greater antioxidant activity in free radical scavenging tests. In addition, the shrimp residue extract dramatically reduced the dosage of norfloxacin (up to 87.5%) and gentamicin (up to 75.0%) antibiotics in tests to inhibit the growth of gram-negative bacteria.

Biological Extraction, HPLC Quantification and Medical Applications of Astaxanthin Extracted from Crawfish “Procambarus clarkii” Exoskeleton By-Product

“…In this study, the antimicrobial activity of astaxanthin extracted by S. cerevisiae was studied on a wide range of Gram-positive, Gram-negative bacteria and fungal strains, and our sample was effective against Candida albicans (ATTC: 10231). According to Santos et al [ 43 ], the shrimp residue extract had little antimicrobial activity against Staphylococcus aureus. Dalei et al [ 44 ] observed that the astaxanthin extracted by methanol has the highest antimicrobial activity against Escherichia coli, Bacillus , Pseudomonas , and Staphylococcus.…”

Optimization of the extraction process of flavonoids from Trollius ledebouri with natural deep eutectic solvents

“…In addition, NADESs has the advantages of easy preparation, easy storage, sustainability, and biodegradability [18]. NADESs are considered to be an effective and safe solvents for extracting bioactive components [19][20][21], RNA [22], protein [23,24], and purified fuel [25]. It is also used in other popular and emerging scientific fields, such as organometallic [26] and biocatalysis [27].…”