Device for Streptavidin Detection Using LSPR and Electrochemical Transductions on the same Platform

Abstract

Research in biosensing currently occupies an important role among biomolecular detection techniques, especially with respect to medical diagnoses. Is this sense, the development of biosensors based on the localized surface plasmon resonance (an optical transducer) or electrochemical transductions have been highlighted. However, optical and electrochemical detection working together enable more versatile detections in biosensing. Thus, we propose a dual sensor, which incorporates these two mechanisms on the same platform. The sensor shared the same transducer that was based on a glass-indium thin oxide (ITO) surface covered with gold nanoparticles. The transducer was prepared by the immobilization of gold nanoparticles on (3-aminopropyl)triethoxysilane-activated glass-ITO. The substrate ability to respond to physical surface variations was assessed. For this, measurements of the gold nanoparticles band redshift (plasmonic transduction) and charge transfer resistance from impedance spectra (electrochemical transduction) were performed. The platform was able to detect streptavidin immobilized on the surface by both mechanisms demonstrating its applicability as a device for biodetection in dual detection mode.

Methylene Blue functionalized carbon nanodots combined with different shape gold nanostructures for sensitive and selective SARS-CoV-2 sensing

“…Among nanomaterials, gold nanomaterials are considered as perfect materials for electrochemical biosensors consequence of their properties such as high conductivity, high surface area, catalytic activity and biocompatibility [29] , [30] . Despite the considerable interest demonstrated for gold nanostructures of different shapes in electrochemical DNA biosensor development, particularly, spherical gold nanoparticles [31] , [32] , and the differences that the shape causes in the behaviour, the combination of gold nanomaterials of different shapes has not been widely exploited yet. This combination of gold nanomaterials of different shapes can provide many advantages.…”