Pueraria lobata Root Constituents as Xanthine Oxidase Inhibitors and Protective Agents against Oxidative Stress Induced in GES-1 Cells

Tang, Xiaosheng; Xiao, Aiping; Mei, Shuqi; Tang, Ping; Ren, Lixia; Liu, Liangliang

doi:10.21577/0103-5053.20200108

Cited by 4 publications

(4 citation statements)

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“…The average binding energy of XO-daidzin was lower than that of XO-puerarin, which were −77.58 ± 1.18 kJ/mol and −53.65 ± 1.19 kJ/mol, respectively. In the existing experimental study, Dr. Tang et al determined the IC50 values of puerarin and daidzin on XO inhibition, which were 30.8 and 5.31 μg mL –1 , respectively . Other researchers have also reported the XO inhibition effects of these two compounds. , At the same time, Dr. Tang et al also proved that the quenching mode of daidzin and puerarin combined with XO can be considered as static quenching.…”

Section: Resultsmentioning

confidence: 97%

Molecular Dockings and Molecular Dynamics Simulations Reveal the Potency of Different Inhibitors against Xanthine Oxidase

Pan

et al. 2021

ACS Omega

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Xanthine oxidase (XO), which can catalyze the formation of xanthine or hypoxanthine to uric acid, is the most important target of gout. To explore the conformational changes for inhibitor binding, molecular dockings and molecular dynamics simulations were performed. Docking results indicated that three inhibitors had similar pose binding to XO. Molecular dynamics simulations showed that the binding of three inhibitors influenced the secondary structure changes in XO. After binding to the inhibitor, the peptide Phe798-Leu814 formed different degrees of unhelix, while for the peptide Glu1065-Ser1075, only a partial helix region was formed when allopurinol was bound. Through the protein structure analysis in the simulation process, we found that the distance between the active residues Arg880 and Thr1010 was reduced and the distance between Glu802 and Thr1010 was increased after the addition of inhibitors. The above simulation results showed the similarities and differences of the interaction between the three inhibitors binding to the protein. MM-PBSA calculations suggested that, among three inhibitors, allopurinol had the best binding effect with XO followed by daidzin and puerarin. This finding was consistent with previous experimental data. Our results can provide some useful clues for further gout treatment research.

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Section: Resultsmentioning

confidence: 97%

Molecular Dockings and Molecular Dynamics Simulations Reveal the Potency of Different Inhibitors against Xanthine Oxidase

Pan

et al. 2021

ACS Omega

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“…It has been reported in our laboratory and in the literature that polyphenols in MS extracts reduce the concentration of ROS produced by oxidative stress, as is the case with resveratrol [ 78 ] and pyrogallol [ 79 ]. Daidzein and diadzin can reduce ROS concentrations and LDH release in response to oxidative damage, raising the activity of the enzyme superoxide dismutase and lowering the expression of COX-2 and NF-κβ [ 64 , 80 , 81 , 82 ]. Thus, when HDFa cells were pre-incubating with 100 µg/mL RAE and then infected with S. aureus , a 64% reduction in ROS formation was observed relative to infected controls ( Figure 5 B,C).…”

Section: Discussionmentioning

confidence: 99%

Chemistry and Bioactivity of Microsorum scolopendria (Polypodiaceae): Antioxidant Effects on an Epithelial Damage Model

et al. 2022

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Microsorum scolopendia (MS), which grows on the Chilean island of Rapa Nui, is a medicinal fern used to treat several diseases. Despite being widely used, this fern has not been deeply investigated. The aim of this study was to perform a characterization of the polyphenolic and flavonoid identity, radical scavenging, antimicrobial, and anti-inflammatory properties of MS rhizome and leaf extracts (RAE and HAE). The compound identity was analyzed through the reversed-phase high-performance liquid chromatography (RP-HPLC) method coupled with mass spectrometry. The radical scavenging and anti-inflammatory activities were evaluated for DPPH, ORAC, ROS formation, and COX inhibition activity assay. The antimicrobial properties were evaluated using an infection model on Human Dermal Fibroblast adult (HDFa) cell lines incubated with Staphylococcus aureus and Staphylococcus epidermidis. The most abundant compounds were phenolic acids between 46% to 57% in rhizome and leaf extracts, respectively; followed by flavonoids such as protocatechic acid 4-O-glucoside, cirsimaritin, and isoxanthohumol, among others. MS extract inhibited and disaggregated the biofilm bacterial formed and showed an anti-inflammatory selective property against COX-2 enzyme. RAE generated a 64% reduction of ROS formation in the presence of S. aureus and 87.35% less ROS in the presence of S. epidermidis on HDFa cells. MS has great therapeutic potential and possesses several biological properties that should be evaluated.

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“…It accounted for 15.81% of the constituents in an extract of Puerariae flos and was the component of the extract with the greatest ability to inhibit XO (the other components are tectoridin, ononin, and biochanin A) (Liu et al., 2017). Genistein and daidzein are the main active substances in soybean okara, black bean and P. lobata roots, and potential functional ingredients that may act as natural therapeutic agents for conditions involving XO (Nile et al., 2020; Niu et al., 2022; Tang et al., 2020).…”

Section: Mechanism Of Xo Activity and Hua Inhibition By Flavonoidsmentioning

confidence: 99%

“…For example, an ethanol extract of the rhizomes of Alpinia officinarum with high levels of flavonoids (galangin, kaempferide, and 3‐methoxyl‐galangin) was reported to significantly inhibit XO activity, reduce urea nitrogen and creatinine levels, and alleviate oxidative stress in hyperuricemic rats, thereby significantly decreasing the level of uric acid and protecting the kidneys (Lin, Liu, & Zhao, 2018 ). Genistein, daidzin, daidzein, and puerarin isolated from Pueraria lobata roots showed strong inhibitory effects on XO, with half‐maximal inhibitory concentration (IC 50 ) values of 3.02, 5.31, 14.5, and 30.8 μg/mL, and exhibited protective effects on the oxidative stress model of human gastric epithelial cells, improving cell viability and reducing mortality (Tang et al., 2020). In addition, a large number of flavonoids with inhibitory effects on XO have been found in Sudanese roselle , sunflower, and shallot (El‐Naeem et al., 2022; Mehmood et al., 2018; Nguyen Thu et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

Mechanism of flavonoids inhibiting xanthine oxidase and alleviating hyperuricemia from structure–activity relationship and animal experiments: A review

Xue

Gong

et al. 2023

Food Frontiers

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Hyperuricemia (HUA) is a metabolic disease caused by excessive uric acid in patients. Flavonoids have attracted extensive attention due to their safety, effectiveness, and little side effects in the treatment of HUA and inhibition of xanthine oxidase (XO) activity. In this paper, the studies on the treatment of HUA and the inhibition of XO activity by flavonoids (including flavones, flavonols, flavanones, flavanols, isoflavonoids, anthocyanins, and chalcones) were comprehensively reviewed from the aspects of structure–activity relationship and animal experiments. Results showed that hydrogen bonds and hydrophobic interactions were the two most important forces involved in the interaction between flavonoids and XO. Flavonoids could combine with the hydrophobic cavity of XO to cause changes in XO structure and influence the action of enzyme and substrate, thereby decreasing the catalytic activity of XO. Moreover, flavonoids undergo a series of metabolic reactions in the organism, which plays a role in alleviating HUA by affecting the expression of related genes and proteins (e.g., glucose transporter 9, urate transporter 1, and organic anion transporter 1) in the kidneys, liver, and intestinal tract of the organism and changing the internal environment in organs. In addition, this paper provides new perspectives on the shortcomings of the current research, to promote the in‐depth development and application of flavonoids.

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Pueraria lobata Root Constituents as Xanthine Oxidase Inhibitors and Protective Agents against Oxidative Stress Induced in GES-1 Cells

Cited by 4 publications

References 0 publications

Molecular Dockings and Molecular Dynamics Simulations Reveal the Potency of Different Inhibitors against Xanthine Oxidase

Molecular Dockings and Molecular Dynamics Simulations Reveal the Potency of Different Inhibitors against Xanthine Oxidase

Chemistry and Bioactivity of Microsorum scolopendria (Polypodiaceae): Antioxidant Effects on an Epithelial Damage Model

Mechanism of flavonoids inhibiting xanthine oxidase and alleviating hyperuricemia from structure–activity relationship and animal experiments: A review

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