Cocoa Agro-Industrial Residue (Theobroma cacao) as Inducer of the Production of Fungal Laccase and Kojic Acid for Application in the Biodegradation of 17-α-Ethinylestradiol

Golveia, Jhéssica Cavalcante de Souza; Bara, Maria Teresa Freitas; Santiago, Mariângela Fontes; Campos, Luiza C.; Schimidt, Fernando

doi:10.21577/0103-5053.20200102

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Attributions of the chemical structures of annotated compounds were supported by MS 2 (Figure 5) data analysis and proposition of the fragmentation pathways of [M−H] − from all compounds (Scheme 1). The careful analysis of MS 2 data (Figure 5A) suggested that kojic acid ( 1 ) fragmented to generate the m / z 141, 113, 95, and 65 ions, which coincided with the fragmentation pattern previously reported for such compound [25,26] . Figures 5B and 5 C show MS 2 spectra from haliclonol ( 2 ) and its diastereoisomer ( 3 ), respectively.…”

Section: Resultssupporting

confidence: 82%

“…The careful analysis of MS 2 data (Figure 5A) suggested that kojic acid (1) fragmented to generate the m/z 141, 113, 95, and 65 ions, which coincided with the fragmentation pattern previously reported for such compound. [25,26] Figures 5B and 5 C show MS 2 spectra from haliclonol (2) and its diastereoisomer (3), respectively. Comparison of both spectra shows no differences, which impossibilities the stereochemistry proposition of compound 3.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Untargeted Metabolomic to Access Chemical Differences Induced by Dual Endophyte Cultures Isolated from Euphorbia Umbellata

Santos Gusmão,

Silva Conceição,

Moreira de Queiros Santos

et al. 2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

Endophytic fungi live asymptomatically inside vegetal tissues, and such uncommon habitat contributes to their exceptional chemical diversity. Isolating natural products from endophytic fungi could fail due to silent biosynthetic gene clusters under ordinary in vitro culture conditions, and co‐culturing has been assayed to trigger their metabolism. We carried out single and dual cultures with 13 endophyte strains isolated from Euphorbia umbellata leaves. Multivariate statistics applied to untargeted metabolomics compared the chemical profiles of all endophyte cultures. PCA analysis guided the selection of the Aspergillus pseudonomiae J1 ‐ Porogramme brasiliensis J9 dual culture for its most significant chemical differentiation: Five compounds were putatively annotated in the J1‐J9 culture according to UHPLC‐HRMS data, kojic acid, haliclonol and its diastereoisomer, caffeic acid, and 2‐(3,4‐dihydroxyphenyl)acetaldehyde. Analysis by PLS‐DA using VIP score showed that kojic acid displayed the most significative importance in discriminating single and dual J1‐J9 cultures.

show abstract

Section: Resultssupporting

confidence: 82%

Section: Resultsmentioning

confidence: 99%

Untargeted Metabolomic to Access Chemical Differences Induced by Dual Endophyte Cultures Isolated from Euphorbia Umbellata

Santos Gusmão,

Silva Conceição,

Moreira de Queiros Santos

et al. 2024

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…To ensure the selectivity of the biosensor, enzymes were often used as electrocatalysts [12]. Lactate dehydrogenase (LDH) and laccase (Lac) were usually used in the process of catalyzing lactate [13]. LDH had highly stable and promotes the transition of lactate to pyruvate and nicotinamide adenine dinucleotide (NAD + ), it was widely used in lactate biosensors [12,14,15].…”

Section: Introductionmentioning

confidence: 99%

Wearable cellulose textile matrix self-powered biosensor sensing lactate in human sweat

夏,

Zuo,

et al. 2023

Preprint

View full text Add to dashboard Cite

A lactate self-powered biosensor was assembled for the detection of lactate in sweat based on flexible textile matrix enzymatic electrodes. A porous three-dimensional electrode with high flexibility and electrical conductivity was obtained by decorating composites of reduced graphene oxide and carboxylate multi-walled carbon nanotubes onto a cellulose fabric substrate. The excellent enzyme embedding method using gel electrolyte showed a Michaelis-Menten constant of 1.46 mM, which implies that lactate dehydrogenase had a high enzymatic activity. The assembled self-powered lactate biosensor showed a sensitivity of 3.16 µW mM− 1 cm− 2 in the 0–10 mM linear range and a detection limit of 9.49 µM (S/N = 3). Meanwhile, the biosensor has good tensile flexural stability, selectivity, and long-term stability. The lactate self-powered biosensor recovered 99.66–108.43% of lactate from real sweat with a relative standard deviation of less than 4.65% when integrated into clothing. The biosensor is promising for sensing lactate in real sweat.

show abstract

“…These residues cannot be disposed of on the soil without any treatment because they may act as promoter to Phytophthora spp., the main cause of black pod rot [3]. Indeed, different studies have presented alternatives for using CPH residues such as anaerobic digestion [4], high value chemicals precursors [5][6][7], pectin extraction [8], and ash used as fertilizer [9,10], among others. Thus, for example, CPH was studied as a source of renewable energy through anaerobic digestion, where it has been possible to recover 54% of the chemical oxygen demand of the waste in the form of methane, being able to produce 8341 MWh annually.…”

Section: Introductionmentioning

confidence: 99%

Study of Cocoa Pod Husks Thermal Decomposition

et al. 2022

View full text Add to dashboard Cite

Thermal decomposition of cocoa pod husks under inert and oxidizing atmospheres was studied. Samples from Cotopaxi, Ecuador were used as raw material. Thermogravimetry based experiments were used to obtain decomposition data vs. temperature. A novel strategy to fit the TG and DTG curves was used giving good fit by considering, in the kinetic model, four biomass fractions following independent reactions. Analytical pyrolysis was used to determine the composition of volatile compounds obtained in slow (150–350, 350–500 °C) and flash pyrolysis (400 °C). The results indicate that in the slow pyrolysis experiments at low temperatures (150–350 °C), the highest area percentages correspond to ketones (7.5%), organic acids (12.5%) and phenolic derivatives (10%), while at increased temperatures (350–500 °C) the higher percentages are clearly focused on phenolic derivatives (12%) and aromatic compounds (10%). Comparing the results of flash pyrolysis at 400 °C (i.e., higher heating rate but lower final temperature), an increase in the yield of ketones and organic acids is observed compared to slow pyrolysis, but the percentage of phenols and aromatics decreases. The results obtained allow deducing the operating conditions to maximize the mass fraction of the different functional groups identified.

show abstract

Cocoa Agro-Industrial Residue (Theobroma cacao) as Inducer of the Production of Fungal Laccase and Kojic Acid for Application in the Biodegradation of 17-α-Ethinylestradiol

Cited by 4 publications

References 0 publications

Untargeted Metabolomic to Access Chemical Differences Induced by Dual Endophyte Cultures Isolated from Euphorbia Umbellata

Untargeted Metabolomic to Access Chemical Differences Induced by Dual Endophyte Cultures Isolated from Euphorbia Umbellata

Wearable cellulose textile matrix self-powered biosensor sensing lactate in human sweat

Study of Cocoa Pod Husks Thermal Decomposition

Contact Info

Product

Resources

About