Analysis of the Gene Expression and RNAi-Mediated Knockdown of Chitin Synthase from Leaf-Cutting Ant Atta sexdens

β‐Chitin and chitosan from waste shells of edible mollusks as a functional ingredient

“…This difference in chitin synthesis can be identified by exploring the enzyme chitin synthase (CHS). RNAi‐mediated silencing of the CHS gene in the insect Atta sexdens results in its downregulation, which leads to morphological modification and malfunction in chitin synthesis (Moreira et al., 2020). More exploration into the CHS gene was needed by using the recent advancements in genomics and genetics tools such as Sanger sequencing, whole‐genome sequencing (WGS), transcriptome, recombinant DNA technology, and in silico bioinformatics analysis.…”

“…Furthermore, the genomic and genetics‐based approach of selective breeding of high chitin‐producing mussels can be implemented by (1) mussels free of mutation in the CHS gene and the other genes involved in the chitin pathway, (2) expression level of chitin pathway genes in mussels, and (3) also identification of other potential genomic markers. Genomic tools such as WGS, genetic linkage maps, genetic marker panels, and identification of quantitative trait loci were already recommended in the selective breeding of mollusks species for improving the key production traits (Moreira et al., 2020). In this case, key production traits can be high chitin production.…”

“…downregulation, which leads to morphological modification and malfunction in chitin synthesis (Moreira et al, 2020). More exploration into the CHS gene was needed by using the recent advancements in genomics and genetics tools such as Sanger sequencing, whole-genome sequencing (WGS), transcriptome, recombinant DNA technology, and in silico bioinformatics analysis.…”