Assessing the Spatial Distribution of Key Flavonoids in Mentha × piperita Leaves: An Application of Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI)

Freitas, Julia Raquel Lino e; Vendramini, Pedro Henrique; Melo, Júlio Onésio Ferreira; Eberlin, Marcos N.; Augusti, Rodinei

doi:10.21577/0103-5053.20190039

Cited by 12 publications

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“…Nowadays, there have been increasing reports on the applications of mass spectrometry imaging (MSI) in the investigation of animal or human tissues ( Wu et al, 2019) , but with less focus on plants ( Davison et al, 2019 ; Sun et al, 2020 ; Zhang et al, 2020d) , not to mention traditional Chinese medicine (TCM). In addition, most of the research works were performed with the fresh herbs ( Fowble et al, 2017 ; Lange et al, 2017 ; Freitas et al, 2019) due to the obstacles in the sectioning of the dried material. To reveal the spatial localization of phytochemicals in traditional Chinese medicine (TCM) in the form of actually sold in the market and used in clinic, the dried root of Isatis tinctoria was chosen as the imaging subject.…”

Section: Discussionmentioning

confidence: 99%

Microscopic Mass Spectrometry Imaging Reveals the Distribution of Phytochemicals in the Dried Root of Isatis tinctoria

et al. 2021

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The dried root of Isatis tinctoria L. (Brassicaceae) is one of the most popular traditional Chinese medicines with well-recognized prevention and treatment effects against viral infections. Above 300 components have been isolated from this herb, but their spatial distribution in the root tissue remains unknown. In recent years, mass spectrometry imaging (MSI) has become a booming technology for capturing the spatial accumulation and localization of molecules in fresh plants, animal, or human tissues. However, few studies were conducted on the dried herbal materials due to the obstacles in cryosectioning. In this study, distribution of phytochemicals in the dried root of Isatis tinctoria was revealed by microscopic mass spectrometry imaging, with application of atmospheric pressure–matrix-assisted laser desorption/ionization (AP-MALDI) and ion trap–time-of-flight mass spectrometry (IT-TOF/MS). After optimization of the slice preparation and matrix application, 118 ions were identified without extraction and isolation, and the locations of some metabolites in the dried root of Isatis tinctoria were comprehensively visualized for the first time. Combining with partial least square (PLS) regression, samples collected from four habitats were differentiated unambiguously based on their mass spectrometry imaging.

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Section: Discussionmentioning

confidence: 99%

Microscopic Mass Spectrometry Imaging Reveals the Distribution of Phytochemicals in the Dried Root of Isatis tinctoria

et al. 2021

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“…Another sample preparation strategy used in our studies for imaging was the imprinting procedure 31 ; however, we chose to perform the direct analysis in the leaves tissue due to the similar results obtained in both strategies. The direct analysis led to almost zero sample preparation and faster diagnostics.…”

Section: Resultsmentioning

confidence: 99%

“…MSI analyses are suitable for complex surfaces because it allows the detection of hundreds to thousands of compounds, such as lipids, proteins, and low molecular weight molecules. Furthermore, MSI indicates the spatial distribution of compounds in the sample surface 28 – 31 .…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry imaging as a potential technique for diagnostic of Huanglongbing disease using fast and simple sample preparation

Pontes

Vendramini

Fernandes

et al. 2020

Sci Rep

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Huanglongbing (HLB) is a disease of worldwide incidence that affects orange trees, among other commercial varieties, implicating in great losses to the citrus industry. The disease is transmitted through Diaphorina citri vector, which inoculates Candidatus Liberibacter spp. in the plant sap. HLB disease lead to blotchy mottle and fruit deformation, among other characteristic symptoms, which induce fruit drop and affect negatively the juice quality. Nowadays, the disease is controlled by eradication of sick, symptomatic plants, coupled with psyllid control. Polymerase chain reaction (PCR) is the technique most used to diagnose the disease; however, this methodology involves high cost and extensive sample preparation. Mass spectrometry imaging (MSI) technique is a fast and easily handled sample analysis that, in the case of Huanglongbing allows the detection of increased concentration of metabolites associated to the disease, including quinic acid, phenylalanine, nobiletin and sucrose. The metabolites abieta-8,11,13-trien-18-oic acid, suggested by global natural product social molecular networking (GNPS) analysis, and 4-acetyl-1-methylcyclohexene showed a higher distribution in symptomatic leaves and have been directly associated to HLB disease. Desorption electrospray ionization coupled to mass spectrometry imaging (DESI-MSI) allows the rapid and efficient detection of biomarkers in sweet oranges infected with Candidatus Liberibacter asiaticus and can be developed into a real-time, fast-diagnostic technique.

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“…By pressing the plant samples onto flat industrial materials under manual or mechanical forces, metabolites in original plant tissues are transferred to the flat surface, which is the so-called “imprint”. Imprints of plant tissues could be made on different materials, such as polymer membranes [ 99 ] and silica gel plates [ 100 ]. The obtained imprint is then analyzed with MSI, which indirectly reflects how compounds are distributed in original sample tissues.…”

Section: Main Textmentioning

confidence: 99%

The application of mass spectrometry imaging in traditional Chinese medicine: a review

et al. 2022

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Mass spectrometry imaging is a frontier technique which connects classical mass spectrometry with ion imaging. Various types of chemicals could be visualized in their native tissues using mass spectrometry imaging. Up to now, the most commonly applied mass spectrometry imaging techniques are matrix assisted laser desorption ionization mass spectrometry imaging, desorption electrospray ionization mass spectrometry imaging and secondary ion mass spectrometry imaging. This review gives an introduction to the principles, development and applications of commonly applied mass spectrometry imaging techniques, and then illustrates the application of mass spectrometry imaging in the investigation of traditional Chinese medicine. Recently, mass spectrometry imaging has been adopted to explore the spatial distribution of endogenous metabolites in traditional Chinese medicine. Data collected from mass spectrometry imaging can be further utilized to search for marker components of traditional Chinese medicine, discover new compounds from traditional herbs, and differentiate between medicinal plants that are similar in botanical features. Moreover, mass spectrometry imaging also plays a role in revealing the pharmacological and toxicological mechanisms of traditional Chinese medicine.

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Assessing the Spatial Distribution of Key Flavonoids in Mentha × piperita Leaves: An Application of Desorption Electrospray Ionization Mass Spectrometry Imaging (DESI-MSI)

Cited by 12 publications

References 0 publications

Microscopic Mass Spectrometry Imaging Reveals the Distribution of Phytochemicals in the Dried Root of Isatis tinctoria

Microscopic Mass Spectrometry Imaging Reveals the Distribution of Phytochemicals in the Dried Root of Isatis tinctoria

Mass spectrometry imaging as a potential technique for diagnostic of Huanglongbing disease using fast and simple sample preparation

The application of mass spectrometry imaging in traditional Chinese medicine: a review

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