Determination of Short Chain Fatty Acids in Mice Feces by Capillary Electrophoresis

Marques, Leila Soares; Cazarin, Cínthia Baú Betim; Bicas, Juliano Lemos; Maróstica, Mário Roberto; Carrilho, Emanuel; Bogusz, Stanislau

doi:10.21577/0103-5053.20190031

Cited by 6 publications

(5 citation statements)

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“…SCFAs are the major gut microbial metabolites of non-digestible carbohydrate fermentation by the gut microbiota . It has been reported that supplementation of probiotics, including Ruminococcus, Bifidobacterium, Eubacterium, and Clostridium, significantly improved the SCFA formation in various animal and human research. − Consistently, in the current research, high-dietary fiber intake also enhanced the levels of Lactobacillus and Bifidobacterium in postpartum mice. Meanwhile, correlation analysis indicated that the relative abundance of these microbes was positively correlated with the SCFA formation.…”

Section: Discussionsupporting

confidence: 77%

High-Dietary Fiber Intake Alleviates Antenatal Obesity-Induced Postpartum Depression: Roles of Gut Microbiota and Microbial Metabolite Short-chain Fatty Acid Involved

Liu

et al. 2020

J. Agric. Food Chem.

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Antenatal obesity increases the risk of postpartum depression. Previous research found that dietary fiber supplementation could alleviate mental behavioral disorders. The present study aims to uncover the effects of high-dietary fiber intake on high-fat diet (HFD)-induced depressive-like behaviors and its underlying mechanism. Female C57BL6/J mice were fed with HFD to establish an antenatal obese model. A high-dietary fiber intake (inulin, 0.037 g/kcal) significantly attenuated cognitive deficits and depressive-like behaviors in the maternal mice after the offspring weaning. High-dietary fiber intake upregulated the expression of 5-hydroxytryptamine (5-HT) and norepinephrine (NE) and suppressed neuroinflammation. Furthermore, high-dietary fiber intake restructured the gut microbiome and elevated the formation of short-chain fatty acids (SCFAs). Correlation analysis indicated that the increase in microbes such as Lactobacillus and S24-7, and SCFAs’ levels were positively correlated with behavioral improvements. In conclusion, high-dietary fiber intake is a promising nutritional intervention strategy to prevent antenatal obesity-induced behavioral disorders via a microbiota–gut–brain axis.

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Section: Discussionsupporting

confidence: 77%

High-Dietary Fiber Intake Alleviates Antenatal Obesity-Induced Postpartum Depression: Roles of Gut Microbiota and Microbial Metabolite Short-chain Fatty Acid Involved

Liu

et al. 2020

J. Agric. Food Chem.

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“…SCFAs are compounds produced during fermentation in the gut microbiota. Acetic, propionic, and butyric acids are the most important SCFAs produced from non-digestible foods [ 24 ]. These three acids act by preserving gut barrier functions, and their anti-inflammatory and immunomodulatory effects [ 7 ], which are severely mitigated in infectious diarrhea.…”

Section: Discussionmentioning

confidence: 99%

Zinc Deficiency Elevates Fecal Protein, But Not Electrolyte and Short-Chain Fatty Acid, Levels in Enterotoxigenic Escherichia coli-Induced Diarrhea in Rats

David

Yameen

Igwenyi

et al. 2022

Pediatr Gastroenterol Hepatol Nutr

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Purpose To determine the effect of zinc deficiency on fecal protein, electrolyte, and short-chain fatty acid levels in both heat-stable (ST) and heat-labile (LT) enterotoxigenic Escherichia coli (ETEC)-induced diarrhea in rats. Methods Albino rats, weighing 100 to 150 g, were divided into 2 groups, with 15 animals each: non-zinc and zinc-deficient. These two groups were sub-divided into three sub-groups with five rats each: control (saline); LT-ETEC; and ST-ETEC. Sodium phytate (30 mmol/L) was added to the animals’ water to induce zinc deficiency, while diarrhea was induced using 5×10 9 ETEC cells/mL. Fecal protein levels were estimated using the Bradford method, while sodium and potassium levels were determined using atomic absorption spectrophotometry. Short-chain fatty acids were measured using gas chromatography-mass spectrometry. Results Among the non-zinc and zinc-deficient groups, there were significant increases ( p =0.04), ( p =0.03) in fecal protein concentrations (mg/mL) in the LT-ETEC- (4.50±0.33), (6.50±0.26) and ST-ETEC- (3.85±0.19), (5.98±0.32) induced groups compared to the control groups (2.60±0.52), (3.50±0.11) respectively. Fecal sodium and potassium levels (mg/L) were significantly ( p =0.029) increased in non-zinc-deficient rats induced with LT-ETEC (9.35±0.95, 1.05±0.48), and ST-ETEC (9.96±1.02, 1.21±0.45) compared with the control group (8.07±0.44, 0.47±0.17) but the increase were not statistically significant ( p =0.059) in the zinc deficient rat groups. Fecal acetate and propionate levels (mg/g) significantly ( p =0.032) increased when induced with LT-ETEC and ST-ETEC in non-zinc and zinc-deficient groups compared with the control groups. Conclusion Zinc deficiency among rats with ETEC-induced diarrhea elevated fecal protein loss but may not have an effect on fecal sodium, potassium and short-chain fatty acid levels.

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“…A rapid and low-cost CE method with indirect UV detection for the determination of three SCFAs in mice faeces was introduced by Marques et al. ( 182 ). The method was applied to identify SCFAs concentration changes in mice with dextran sulfate sodium-induced colitis undergoing the treatment with Plinia caulifora aqueous extract.…”

Section: Analysis Of Scfasmentioning

confidence: 99%

Determination of short-chain fatty acids as putative biomarkers of cancer diseases by modern analytical strategies and tools: a review

et al. 2023

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Short-chain fatty acids (SCFAs) are the main metabolites produced by bacterial fermentation of non-digestible carbohydrates in the gastrointestinal tract. They can be seen as the major flow of carbon from the diet, through the microbiome to the host. SCFAs have been reported as important molecules responsible for the regulation of intestinal homeostasis. Moreover, these molecules have a significant impact on the immune system and are able to affect inflammation, cardiovascular diseases, diabetes type II, or oncological diseases. For this purpose, SCFAs could be used as putative biomarkers of various diseases, including cancer. A potential diagnostic value may be offered by analyzing SCFAs with the use of advanced analytical approaches such as gas chromatography (GC), liquid chromatography (LC), or capillary electrophoresis (CE) coupled with mass spectrometry (MS). The presented review summarizes the importance of analyzing SCFAs from clinical and analytical perspective. Current advances in the analysis of SCFAs focused on sample pretreatment, separation strategy, and detection methods are highlighted. Additionally, it also shows potential areas for the development of future diagnostic tools in oncology and other varieties of diseases based on targeted metabolite profiling.

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Determination of Short Chain Fatty Acids in Mice Feces by Capillary Electrophoresis

Cited by 6 publications

References 0 publications

High-Dietary Fiber Intake Alleviates Antenatal Obesity-Induced Postpartum Depression: Roles of Gut Microbiota and Microbial Metabolite Short-chain Fatty Acid Involved

High-Dietary Fiber Intake Alleviates Antenatal Obesity-Induced Postpartum Depression: Roles of Gut Microbiota and Microbial Metabolite Short-chain Fatty Acid Involved

Zinc Deficiency Elevates Fecal Protein, But Not Electrolyte and Short-Chain Fatty Acid, Levels in Enterotoxigenic Escherichia coli-Induced Diarrhea in Rats

Determination of short-chain fatty acids as putative biomarkers of cancer diseases by modern analytical strategies and tools: a review

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