Simultaneous Multienzymatic Screening with Fluorogenic Probes

Lima, Maria L. S. O.; Chaves, Michel R. B.; Nascimento, Renato M. C. do; Gonçalves, Caroline da Costa Silva; Marsaioli, Anita Jocelyne

doi:10.21577/0103-5053.20170188

Cited by 3 publications

(3 citation statements)

References 34 publications

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“…A strain of T. longibrachiatum with lipase activity was isolated from oil contaminated soil [37]. While CBMAI 2082 showed 89.97% of conversion of the lipophilic probes after 24 h of analysis (Table 3), Lima et al [77] obtained conversion percentages of 0.4 and 4.3% in isolates of Hyphomycetes and Dematiaceous, respectively, working with fluorogenic probes to assess lipases activity at the same time of experiment. This demonstrates how promising our strain can be considered.…”

Section: Qualitative and Quantitative Assays For Lipase And Esterase mentioning

confidence: 99%

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

et al. 2019

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Recalcitrant characteristics and insolubility in water make the disposal of synthetic polymers a great environmental problem to be faced by modern society. Strategies towards the recycling of post-consumer polymers, like poly (ethylene terephthalate, PET) degradation/depolymerization have been studied but still need improvement. To contribute with this purpose, 100 fungal strains from hydrocarbon-associated environments were screened for lipase and esterase activities by plate assays and high-throughput screening (HTS), using short-and long-chain fluorogenic probes. Nine isolates were selected for their outstanding hydrolytic activity, comprising the genera Microsphaeropsis, Mucor, Trichoderma, Westerdykella, and Pycnidiophora. Two strains of Microsphaeropsis arundinis were able to convert 2-3% of PET nanoparticle into terephthalic acid, and when cultured with two kinds of commercial PET bottle fragments, they also promoted weight loss, surface and chemical changes, increased lipase and esterase activities, and led to PET depolymerization with release of terephthalic acid at concentrations above 20.0 ppm and other oligomers over 0.6 ppm. The results corroborate that hydrocarbon-associated areas are important source of microorganisms for application in environmental technologies, and the sources investigated revealed important strains with potential for PET depolymerization.

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Section: Qualitative and Quantitative Assays For Lipase And Esterase mentioning

confidence: 99%

Hydrocarbon-associated substrates reveal promising fungi for poly (ethylene terephthalate) (PET) depolymerization

et al. 2019

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“…Assim, nos ensaios envolvendo sondas fluorogênicas, a sinalização da atividade enzimática resultará de uma série de reações químicas e biológicas desencadeadas pela ação enzimática de interesse (LIMA et al, 2018;GONÇALVES et al, 2016 Os experimentos foram elaborados como segue em ordem de adição e quantidade de soluções:…”

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Photoelectrocatalysis Properties of Cuwo4 Porous Film Under Polychromatic Light

Lima¹,

Reis²,

Costa³

et al. 2019

Estudos Interdisciplinares Nas Ciências Exatas E Da Terra E Engenharias 2

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“…Isto torna possível a sua aplicação na confecção de sondas específicas para detecção única das atividades enzimáticas de epóxido hidrolases, lipases, esterases, fosfatases, álcool desidrogenase e Baeyer-Villiger Monoxigenase (BVMO)(REYMOND et al, 2008). Vale ressaltar que ensaios envolvendo a detecção simultânea da umbeliferona a outro fluoróforo já foram relatados como processos multienzimáticos de detecção.Tal metodologia já torna possível a detecção de mais de uma atividade enzimática simultaneamente(LIMA et al, 2018).…”

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