Commercial and Noncommercial Peroxidases Activity under Ultrasound and Microwave Treatment: a Pretreatment to Improve Wastewater Treatment

Abstract

This study aimed at evaluating the activity of peroxidases after ultrasound and microwave radiation exposure as an alternative to the wastewater treatment improvement. The results showed that after treatment with ultrasound using a power of 30% and a temperature of 55 °C was obtained an increase in relative activity, 129.5% in the enzyme from rice bran, 147.9% in the enzyme from soybean meal and 102.4% in the enzyme from horseradish. Using microwave radiation, the highest relative activity (107.4%) was observed for the peroxidase extracted from rice bran with 10 seconds of reaction time and a reaction temperature of about 50 °C. The data obtained in this study suggest that the ultrasonic bath and microwave are adequate for conducting reactions catalyzed by peroxidases because it was possible to increase their activity during the performed tests. Preliminary tests of enzyme application showed that the highest color removal occurred using 3 mL of the enzyme extract from rice bran and H 2 O 2 concentrations of 40 mg L -1 . This condition resulted in a color removal of about 40%.

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

“…The equipment used was the UNIQUE Ultrasonic Washer, model USC-1800 A, frequency US 40 KHz and maximum power of 137 W (0.42 W cm -²). The tests were performed with ultrasonic bath temperature ranging from 30 to 80 ºC, ultrasonic power ranging from 0 to 100% and with exposure time of 10 to 40 minutes, following experimental design (Adapted from Golunski et al 2017; Adapted from Mulinari et al 2017).…”

“…Aliquots of 3 mL of crude enzyme extract were exposed to microwave, varying conditions according to experimental design. Exposure of the enzyme to microwaves was evaluated in the time range of 5 to 15 minutes, using temperatures between 30 and 80 °C, according to the methodology described by Mazinani, Delong and Yan, (2015) and adapted from Golunski et al (2017). The equipment used was the ANTON PAAR Monowave 100 Microwave Reactor which has unpulsed 500 W output power (ANTON PAAR).…”

“…Some authors point out that the use of microwaves can cause changes in thermodynamic properties, providing increased enzymatic activity (Mazinani, DeLong and Yan 2015;Golunski et al 2017). These ndings suggest that conformational changes in the structure of enzymes occur when exposed to microwaves, possibly due to cleavages that occur during the process and the formation of hot spots by instant heating.…”

Reactional Ultrasonic Systems And Microwave Irradiation For Pretreatment Of Agro-Industrial Waste To Increase Enzymatic Activity

“…The equipment used was the UNIQUE Ultrasonic Washer, model USC-1800 A, frequency US 40 KHz and maximum power of 137 W (0.42 W cm² (−1) ). The tests were performed with ultrasonic bath temperature ranging from 30 to 80 ºC, ultrasonic power ranging from 0 to 100% and with exposure time of 10 to 40 minutes, following experimental design (Mulinari et al 2017).…”

“…The behavior observed for ultrasonic bath may be related to the synergistic effect of ultrasonic waves with temperature. Several studies show increased activity of enzymes exposed to ultrasonic bath (Leaes et al 2013;Mulinari et al 2017). According to the literature, temperature considerably influences the increase or decrease of enzymatic activity.…”