Síntese, Fotorreatividade E Interação Com Dna De Um Novo Complexo De Rutênio Protótipo Para O Desenvolvimento De Agentes Fotoquimioterapêuticos

Abstract

SYNTHESIS, PHOTOREACTIVITY AND INTERACTION WITH DNA OF A NEW PROTOTYPE RUTHENIUM COMPLEX FOR THE DEVELOPMENT OF PHOTOCHEMOTHERAPEUTIC AGENTS. A new ruthenium polypyridine complex, cis-[Ru(bpy)2(1- meimN)(4-pyCHO)](PF6)2 (WP1), where 1-meimN and pyCHO are the ligands 1-methylimidazole and 4-pyridinecarboxaldehyde, respectively, was prepared as a prototype for use in phototherapeutic process. This compound was characterized by electronic absorption in UV-Visible, FTIR, 1H and 13C NMR, cyclic voltammetry, besides DFT that supported its molecular structure. The compound WP1 was photoreactive, where blue or green light promoted the release of 4-pyCHO ligand. Additionally, this compound was able to interact with DNA, mainly through electrostatic interactions showing photoinduced nuclease activity. There is also photoproduction of superoxide ions using blue or green light suggesting a route for DNA damage. These promising results opened new opportunities for the potential use of this system and analogues as phototherapeutic agents, which is an ongoing study in our lab.