Abstract:DETERMINATION OF PHOSPHATIDYLETANOL IN DRIED BLOOD SPOT BY UHPLC-MS/MS: EVALUATION OF ALCOHOL CONSUMPTION IN CHEMICAL DEPENDENTS. We developed and validated an UHPLC-MS/MS method for the quantification of phosphatidylethanol (PEth) in dried blood spots (DBS). Sample preparation was a liquid extraction and chromatographic separation was performed in an Acquity C8 column. The mobile phases were 4 mM ammonium acetate in water (A) and acetonitrile (B) at 24:76 (v/v). The total analytical run time was 6 minutes, wi… Show more
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