CHEMICAL PROFILE AND ANTIOXIDANT ACTIVITY OF GEOPROPOLIS FROM Melipona subnitida COLLECTED INSIDE AND OUTSIDE THE NEST

Abstract

Melipona subnitida (“jandaíra”) is a species of stingless bee endemic to the Caatinga, a dry forest in the northeastern Brazilian semiarid. Propolis is a product containing beeswax and plant resin. It contains compounds that can be able to prevent health problems by protecting cells against damage by oxidative agents. M. subnitida produces geopropolis, a mixture of propolis with soil. Samples of geopropolis of M. subnitida, one of inside and another outside of the nest from a hive in the municipality of Mossoró (northeastern Brazil) were analyzed by HPLC-PDA-ESI-MS/MS and their antioxidant activity evaluated by the DPPH and β-carotene/linoleic acid methods. Both samples exhibited similar chemical profile, characterized by 26 phenolic compounds, however, the compounds detected were more abundant in sample collected from inside of the nest. They were tentatively characterized as galloyl hexoses, coumaroylO-galloyl-hexoses, cinnamoyl-O-galloyl-hexoses, coumaroyl- and cinnamoyl- galloyl hexoses, phenylpropanoyl-O-benzoyl hexoses, flavanones and ellagitannins. Flavonoids, galloyl hexoses, coumaroyl-O-galloyl-hexosides, cinnamoyl-O-galloyl-hexosides and coumaroyl-cinnamoyl-galloyl hexosides were reported previously in this genus, however ellagitannins and phenylpropanoyl-Obenzoyl hexoses are reported for the first time. Comparing the antioxidant activity of the geopropolis samples from inside and outside of the nest, the latter was higher than the former

Chemical Characterization and Biological Activities of Jandaíra Stingless Bee Products (Melipona subnitida, Ducke, 1911): A Brief Review

“…It was reported in the study by Ferreira et al., (2022), [26] the chemical profile of the ethanolic extracts of geopropolis of M. subnitida , by High Performance Liquid Chromatography, coupled to a Diode Array Detector and Mass Spectrometry – HPLC‐DAD‐ESI‐MS/MS. We identified 26 compounds, including gallic acid, galloyl hexoses, one flavone, one chalcone, caffeoyl‐4‐ O ‐glucose, one 1‐ O ‐ p ‐methoxybenzoyl hexose, flavanones, phenylpropanoyl‐ O ‐galloyl hexoses, phenylpropanoyl‐ O ‐benzoyl hexoses and ellagitannins.…”

“…In the study by Ferreira et al, (2022), [26] the antioxidant activity of ethanolic extracts of geopropolis of M. subnitida were carried out through the radical scavenging test of 1,1-diphenyl-2-picrylhydrazyl (DPPH) and β-carotene/linoleic acid. Using the DPPH method, the EC 50 of geopropolis ethanolic extracts showed values of 17.2 μg/mL À 1 and 147.4 μg/mL À 1 .…”

Evaluation of the antifungal activity of propolis extracts from stingless bees on phytopathogenic fungi

“…Studies already carried out on propolis from A. mellifera indicated a very large variation in the composition, both qualitative and quantitative, of the phenolic compounds (Silva et al, 2013;Cardozo et al, 2015;Sousa et al 2019;Ferreira et al, 2020;Letullier et al, 2020;Ferreira et al, 2022). According to Silva et al (2016), studying eight geopropolis samples collected from Plebeia Aff.…”