HIGH RESOLUTION LC-MS/MS SCREENING FOR SECONDARY METABOLITES IN BULGARIAN SPECIES OF GENUS Astragalus L.

Abstract

The phytochemical content of some Astragalus species distributed in Bulgarian flora has been previously studied. Among other compounds, flavoalkaloids, acylated flavonoids, flavonoid triglycosides and cycloartane saponins have been isolated so far. The composition of the rest of the representatives of this genus in Bulgaria is not explored yet. The aim of this study was to perform a screening for the presence of selected rare secondary metabolites (flavoalkaloids, acylated flavonoids, flavonoid triglycosides and cycloartane saponins) in selected Astragalus species. Samples were collected in different phenological stages and from different locations in the country. A novel and rapid ultra-high performance liquid chromatography – high resolution electrospray ionisation mass spectrometry (UHPLC-HRESIMS) method was developed and applied. For the first time a flavoalkaloid glycoside was determined from extracts of A. onobrychis var. chlorocarpus and A. glycyphylloides. From A. depressus an acylated derivative of kaempferol was newly identified. The flavonol triglycosides camelliaside A, alcesefoliside and mauritianin were proved in samples of A. glycyphylloides, A. onobrychis var. chlorocarpus and A. cicer for the first time as well.

Insights into HPLC-MS/MS Analysis, Antioxidant and Cytotoxic Activity of Astragalus fruticosus against Different Types of Cancer Cell Lines

“…galactopyranoside] (QueHMG), which was confirmed by the MS 2 spectrum where the base peak at m/z 609.2 was due to the loss of the rhamnopyranosyl terminal sugar unit. The same peak at m/z 609.1462 was observed in the MS 2 spectrum of QueHMG, identified in A. monspessulanus using high resolution ESI-MS (HRESIMS) [41]. Another peak in the MS 2 [43] and A. trojanus [44], and named as Astrasieversianin XV.…”

“…galactopyranoside] (KaeHMG), a flavonoidal glycoside that was previously identified in A. depressus [41], A. monospessulanus subsp. Illyricus [41] and A. gombiformis [40].…”

“…galactopyranoside] (KaeHMG), a flavonoidal glycoside that was previously identified in A. depressus [41], A. monospessulanus subsp. Illyricus [41] and A. gombiformis [40]. The MS 2 spectrum demonstrated a peak at m/z 593 representing the loss of the terminal rhamnopyranose unit, which was previously documented in the MS 2 spectrum for KaeHMG when the phenolic components of A. gombiformis were analyzed [40].…”

Nutritional Evaluation of Quinoa Genetic Resources Growing in the Climatic Conditions of Central Europe

“…Mauritianin belongs to the group of flavonols. This compound has been well described in the genus Astragalus [ 67 , 68 ], but in quinoa, this compound has only been reported in two studies [ 69 , 70 ]. The potential health effects of this compound have not been well described.…”

Flavonoids in in vitro cultures of Astragalus hamosus

“…Identification of analytes in all samples was done based on retention time, and the mass spectral fragmentation of the compound, compared to the corresponding standard, injected at the same conditions. The retention times and the spectral fragmentation pattern of the reference substances was previously described (Shkondrov and Krasteva 2021a). Results from the identification are presented in Table 1.…”