A Simple and Sensitive Lc-MS/MS Method for the Determination of S-Phenylmercapturic Acid in Human Urine

Validation of Analysis of S-Phenyl Mercapturic Acid in Urine by High-Performance Liquid Chromatography – Photodiode Array

“…3 mL of urine was taken from the sample and sulfuric acid H2SO4 was added at a concentration of 9 N until the medium became pH=1 (This stage is achieved in order to convert the substance from the salt form to its free form "Pre SPMA to SPMA") 21 .…”

“…In order to excretion of SPMA in small amounts, highly sensitive methods like GC-MS and LC-MS should be used to determine SPMA levels 20,21 . However, these methods are not suitable for routine analysis in clinical diagnostics due to their high cost.…”