AVALIAÇÃO DE COMPOSTOS BIOATIVOS E ATIVIDADE ANTIOXIDANTE DE EXTRATOS DE FOLHAS DE AMOREIRA PRETA (Morus nigra L.) UTILIZANDO PLANEJAMENTO EXPERIMENTAL

Abstract

EVALUATION OF BIOATIVE COMPOUNDS AND ANTIOXIDANT ACTIVITY OF EXTRACTS OF BLACK MULBERRY LEAVES (Morus nigra L.) USING EXPERIMENTAL DESIGN. Extracts of black mulberry leaves present phytochemicals such as phenolic compounds and minerals. These substances have been studied because they show several health benefits. In this work we report the influence of drying temperature, infusion temperature and extraction time by means of factorial design monitoring the total phenolic content (CFT) of aqueous extracts of leaves of Morus nigra L. The concentrations of CFT, flavonoids, flavonols and antioxidant activity of the extracts were determined by spectrophotometry. The identification and quantification of phenolic compounds were performed by LC-ESI-MS / MS. The determination of the mineral concentration in the black mulberry leaves was performed by ICP-MS. The Pareto and surface response graphs suggested that the most efficient extractions occurred for leaves drying at lower temperatures and using higher infusion temperatures, extraction time did not show significant influence. The extracts presented concentration of CFT from 16.96 ± 0.15 to 5.73 ± 0.33 mg GAE g-1. The analyzes by LC-ESI-MS / MS allowed to identify phenolic substances not yet reported in the literature to date. In addition 10 mineral elements were determined in the black mulberry leaves at mg kg-1 concentrations range.

In vitro antioxidant extracts evaluation from the residue of the Hevea brasiliensis seed

“…It can be suggested high temperatures contribute to the extraction. And several studies have shown results in which elevated temperatures favor the extraction of antioxidant compounds 35 – 39 .…”

“…The most notable advantages of the soxhlet method are the non-permanent contact of the sample with the solvent, constant renewal, avoiding solvent saturation, and the system temperature remains relatively high, as the heat applied to the evaporation process is continuous. These advantages contribute to higher antioxidant rates extracted in the soxhlet method than the infusion method, which is static and not isothermal 35 – 39 .…”

Efeito do extrato das folhas de amoreira (Morus nigra L.) no processo inflamatório cardiovascular e na resistência insulínica de camundongos dislipidêmicos

“…O material coletado foi transportado até o laboratório da Universidade José do Rosário Vellano (UNIFENAS) e imediatamente higienizado antes da desidratação. As amostras foram desidratadas em estufa de circulação de ar forçado (Fanem, 320-SE) com temperatura regulada para 50° ± 2 °C conforme recomendado por Schafranski et al (2019). Em seguida, as folhas foram moídas em moinho analítico (Quimis®, São Paulo, Brasil) e armazenadas protegidas da luz, em tubos do tipo falcon escuros, e mantidas em temperatura de -6° ± 1 °C até o momento da extração.…”

“…Os compostos fenólicos da folha da amoreira, principalmente os ácidos cafeoilquínicos (Silva, 2012), podem ter contribuído para prevenir a oxidação do HDL e sua remoção hepática (Christison et al, 1996), assim como também por ação direta como antioxidante (turgut, 2015;Dalmagro et al, 2017;Schafranski et al, 2019) no sistema cardiovascular. O papel anti-inflamatório da HDL pode estar relacionado a seu efeito antioxidante (Holvoet et al, 2008;Garcia et al, 2011).…”

Use of ultrasound and acerola (Malpighia emarginata) residue extract tenderness and lipid oxidation of pork meat

“…The extraction of these compounds is essential for studies that prove their composition and antioxidant capacity. Therefore, isolation, quantification, identification of phytochemicals in plant sources of these compounds and evaluation of benefits that these present to health demonstrate the purpose of its use (Schafranski et al, 2019).…”