Metabolômica Microbiana: Inovações E Aplicações

Abstract

MICROBIAL METABOLOMICS: INNOVATIONS AND APPLICATIONS. Over the past few years, metabolomics has been employed in a broad range of applications in different research fields. Microbial metabolomics has been applied in several microbiological areas, such as identification of microorganisms, cell mutations, functional gene approach, identification of unique metabolic pathways and microbial engineering, leading to a better understanding of the global metabolism and metabolic regulation of certain systems. Metabolites produced by microorganisms constitute a large promising group with diverse applications; however, most of these systems are still unknown and underexplored. In this context, the overall analysis of metabolites involved in a biological system can be used to discover potential biomarkers or specific metabolic changes due to a biological phenomenon studied. In this sense, this review aims to understand the main challenges faced by each step of the workflow in the metabolic analysis of microorganisms and how these difficulties have been overcome. A critical analysis of the articles published within the last five years will be accomplished in this paper to understand how these studies have contributed to the microbial metabolomics research. Moreover, major trends observed in this area will be highlighted here in order to guide readers interested in microbial metabolomics in their future researches. Tabela 2. Plataformas e softwares de pré-processamento mais utilizados em metabolômica Nome Website XCMS https://xcmsonline.scripps.edu/ MZmine http://sourceforge.net/projects/mzmine/ OpenMS http://open-ms.sourceforge.net/ MetAlign http://www.metalign.nl MetSign 127

The sustainable cycle of a new cacao-based bioplastic: from manufacturing to exploitable biodegradation products

“…LC-MS-based metabolomics is commonly used in the comparison of different metabolic profiles of biological sample groups. 75 However, metabolomics studies of natural products that may be produced during the biodegradation process of bioplastics is still poorly explored. In this sense, this study sought to identify the metabolites that were produced during the fungal biodegradation process.…”

Metabolomic Strategies to Improve Chemical Information from OSMAC Studies of Endophytic Fungi

“…The metabolomics associated with chemometric tools is a useful tool to get holist chemical information [ 10 ] from microorganisms, resulting in a broad view of metabolite production, as well as assisting the appointments of metabolic pathways, global metabolism of cells, and giving a direction for the selection of metabolites of interest, such as bioactive metabolites for isolation procedures [ 11 ]. In addition, metabolomics can contribute to different research fields, for example, fungal classification and identification, metabolic pathways, the comprehension of plant-fungal chemical interactions, and the discovery of fungal natural products [ 12 ]. However, fungal metabolomics is still under development, and the databases of fungal metabolites remain singular and incomplete [ 9 ].…”

Deep Untargeted Metabolomics Analysis to Further Characterize the Adaptation Response of Gliricidia sepium (Jacq.) Walp. to Very High Salinity Stress

“…Metabolomics (the study of modification in metabolites) is a comprehensive and quantitative analysis of all small molecules in a biological system (Belinato et al, 2019 ). It is one promising approach used to detect and quantify primary and secondary metabolites of low molecular weight, generally <1,500 Da (Bueno and Lopes, 2020 ).…”