Imobilização De Peroxidase De Raiz Forte Em Bagaço De Cana-De-Açúcar

Abstract

IMMOBILIZATION OF HORSERADISH PEROXIDASE ON SUGARCANE BAGASSE. The immobilization of horseradish peroxidase (HRP) on raw and alkaline pre-treated sugarcane bagasse by physical adsorption (ADS) and covalent bond (LC) methods was studied. The saturation of the support with 2 mg of HRP/g of support by LC immobilization reached 35% of immobilization efficiency and 39 units of the immobilized enzyme (U). Regarding the HRP immobilization on sugarcane bagasse without pretreatment and using the same HRP loading, it was observed a reduction in the efficiency of immobilization and in the number of immobilized units for both methods, ADS (13.98% and 15.46 U) and LC (15.79% and 17.46 U). The sugarcane bagasse with alkaline pretreatment experiment, on the other hand, exhibited higher potential for HRP immobilization by LC. The supports and biocatalysts were characterized by Fourier transform infrared spectroscopy (FTIR), showing greater availability of hydroxyl groups in the pretreated support and the typical amide I and amide II bands that corroborate the effectiveness of the enzyme immobilization on sugarcane bagasse. In the same way, the thermogravimetric analysis (TGA) confirmed a higher weight loss in the region I for the derivative immobilized by LC, suggesting the presence of water favored enzymatic activity.

Immobilization of Low-Cost Alternative Vegetable Peroxidase (Raphanus sativus L. peroxidase): Choice of Support/Technique and Characterization

“…For all CF spectra an absorption band at 897 cm −1 was observed. This band is attributed to the C-O-C stretching referring to glycosidic binds between the sugar units of the cellulosic structure [35]. Also, bands at 1160 cm −1 , referring to asymmetrical stretching C-O-C [36], and more intense band in 1028 cm −1 , associated to C-OH stretch vibration, were observed [70].…”

“…The result of immobilization of crude radish peroxidase on the CF support by CB technique was promising (IY: 77.74 ± 0.42%), compared to the literature. Commercial HRP was immobilized on sugarcane bagasse by CB technique obtaining 35% of IY [35]. Bezerra et al [36] immobilized a fungal laccase on coconut fiber obtaining IY of 98%.…”

“…Radish peroxidase was immobilized by physical adsorption (PA) and covalent binding (CB) according to Queiroz et al [35] with minor modifications. Protein loading effect was evaluated (1.3-6.5 mg protein/g support).…”

“…Physical adsorption and covalent binding are consolidated techniques commonly used for immobilization of enzymes on supports [ 34 ]. The literature reports the immobilization of commercial peroxidase (HRP) by covalent binding (CB) and physical adsorption (PA) in a lignocellulosic material—sugarcane bagasse—as organic support [ 35 ]. In the same way, other enzymes were immobilized on coconut fiber [ 36 , 37 , 38 , 39 ], an important bio-residue of world agribusiness [ 40 , 41 ].…”

Ciências Exatas e da Terra e a Dimensão Adquirida através da Evolução Tecnológica 2

“…• Coliformes totais (CT) -teste presuntivo com caldo de sulfato triptose (LST) e teste confirmativo caldo verde brilhante bile 2 % (VB), ambos com período de incubação de 48 h. Para a classificação hidroquímica das amostras, os dados de concentração iônica foram plotados no diagrama de Piper (Fig. 2), com o auxílio do software Qualigraf (2017).…”