CHEMICAL COMPOSITIONS AND ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF PROPOLIS PRODUCED BY Frieseomelitta longipes AND Apis mellifera BEES

Abstract

In this study, we investigated the chemical compositions and antioxidant and antimicrobial activities of propolis produced by the stingless bee Frieseomelitta longipes and the honeybee Apis mellifera collected from colonies in North Brazil. In terms of volatile composition, both mono-and sesquiterpenes were detected in the propolis of F. longipes while only sesquiterpenes were detected in that of A. mellifera. Out of 50 volatiles identified in all samples, 26 were found exclusively in F. longipes propolis and 8 were found exclusively in A. mellifera propolis. The chemical profiles of the propolis extracts were determined by atmospheric pressure chemical ionization-mass spectrometry and liquid chromatography-electrospray ionization-tandem mass spectrometry allowed to identify several prenylated benzophenones. A. mellifera extracts exhibited major antioxidant activity as assessed by the 2,2-diphenyl-1picrylhydrazyl method and all extracts exhibited antioxidant activity as assessed by the β-carotene/linoleic acid method. The ethanolic extracts of the propolis showed promisor activity against all tested microorganisms.

Analysis of bioactive compounds and chemical composition of Malaysian stingless bee propolis water extracts

“…Other than that, phorbol was also identified in Indian honeybee propolis extract for the first time in 2015 ( Ramnath et al 2015 ). However, there are limited studies regarding stingless bee propolis volatiles available compared to honeybees ( Souza et al 2018 ).…”

Active Antioxidant Phenolics from Brazilian Red Propolis: An Optimization Study for Their Recovery and Identification by LC-ESI-QTOF-MS/MS

“…Guttiferone E (Compound 31 ) displayed deprotonated molecular ion at m / z 601.3571. Its MS 2 spectra showed fragment ions at m / z 109.0291 [C 6 O 2 H 5 ] − , 108.0214, 202.9997, 177.0198 [M–H–C 10 H 16 O] − , 335.1285 [ 50 ]. Lastly, compound 32 (with [M–H] − at m / z 601.3670) was tentatively identified as oblongifolin B, yielding a fragment ion at m / z 109.0292.…”

Volatile Compounds, Chemical Composition and Biological Activities of Apis mellifera Bee Propolis

“…In the work of Souza et al [72], A. mellifera extracts, obtained by hydrodistillation, exhibited high antioxidant activity evaluated by free radical DPPH sequestration and β-carotene/linoleic acid methods. The authors linked these results to the presence of the following compounds: prenylated benzophenones, epinemorosone, xanthochymol, gambogenone, and aristophenone A. Wiwekowati et al [73] also attributed the high antioxidant potential of A. mellifera to the structure of its flavonoids and phenolic acids, which was evaluated by inhibition of free radical DPPH.…”