Perfil dos componentes voláteis produzidos pelo fungo fitopatógeno Albonectria rigidiuscula em diferentes condições de cultivo

Abstract

Recebido em 15/12/2016; aceito em 10/04/2017; publicado na web em 13/06/2017 VOLATILE COMPONENTS PROFILE PRODUCED BY THE PHYTOPATHOGENIC FUNGUS Albonectria rigidiuscula IN DIFFERENT CONDITIONS CULTURE. The VOCs produced by the Albonectria rigidiuscula fungus in different conditions were obtained by headspace-solid phase microextraction (HS-SPME) and analyzed by gas chromatography-mass spectrometry (GC-MS). Fourty-four VOCs were identified in different culture media and incubation periods. The highest production of VOCs occurred in solid rice medium and 14 days of incubation. The compounds 3-methylbutan-1-ol, 2-methylbutan-1-ol, γ-muurolene and nerolidol acetate were produced by A. rigidiuscula in all culture conditions. γ-Muurolene was identified as the major compound (40.78%, 26.24% and 27.04%) in PD medium and it was suggested as a chemical marker for this fungus. The use of multivariate data analysis (PCA) and (HCA) allowed the discrimination of the volatile chemical profiles according to the culture medium.

Profile of volatile compounds released by Waitea circinata against Magnaporthe oryzae under different periods and temperatures

“…W. circinata was the largest producer of volatile organic compounds, totaling 19, being eight exclusively on the day four, nine on both days and two on the day eight. According to Oliveira et al (2017), temperature is a variable that influences volatile organic compounds extraction, what was also observed in the present study, reflected in the larger number of volatile organic compounds collected under water bath conditions.…”

“…Without water bath, i.e., at 27 ºC, the plates were perforated, and the fiber was inserted and exposed for extraction. At 50 ºC (submitted to water bath), the plates were initially placed in ethylene glycol (Panreac) for 15 min and stabilized for 5 min, then perforated to insert the fiber, which was exposed for extraction [adapted from Oliveira et al (2017)].…”