The Protective Effect of Kaempferol Against Ischemia/Reperfusion Injury Through Activating SIRT3 to Inhibit Oxidative Stress

Sun, Chuang; Wang, Tingting; Wang, Changying; Zhu, Zhenyin; Wang, Xiaoni; Xu, Jia; An, Huixian

doi:10.21470/1678-9741-2020-0549

Cited by 6 publications

(3 citation statements)

References 30 publications

(27 reference statements)

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“…In the heart, SIRT3 is highly expressed and localized in the mitochondria, where it is essential for the regulation of metabolism and oxidative stress. Studies have demonstrated that SIRT3 overexpression in cardiomyocytes provides cardioprotective responses and highlights its anti‐fibrotic and anti‐inflammation effects 30,32,33 …”

Section: Discussionmentioning

confidence: 99%

“…Studies have demonstrated that SIRT3 overexpression in cardiomyocytes provides cardioprotective responses and highlights its antifibrotic and anti-inflammation effects. 30,32,33 The NLRP3 inflammasome plays an essential role in the pathophysiological process of MIRI after NLRP3 receives exogenous danger and environmental stress signals. 34 NLRP3 oligomerizes to form a wheel-shaped signalling hub, which can exacerbate inflammatory reactions.…”

Section: Discussionmentioning

confidence: 99%

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Therapeutic hypothermia alleviates myocardial ischaemia–reperfusion injury by inhibiting inflammation and fibrosis via the mediation of the SIRT3/NLRP3 signalling pathway

Zhang

et al. 2022

J Cellular Molecular Medi

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Therapeutic hypothermia (TH) may attenuate myocardial ischaemia–reperfusion injury, thereby improving outcomes in acute myocardial infarction. However, the specific mechanism by which TH alleviates MIRI has not been elucidated so far. In this study, 120 healthy male Sprague‐Dawley rats were randomly divided into five groups. Haemodynamic parameters, myocardial infarction area, histological changes and the levels of cardiac enzymes, caspase‐1 and inflammatory cytokines were determined. In addition, the extent of myocardial fibrosis, the degree of cardiomyocyte apoptosis and the expression levels of SIRT3, GSDMD‐N, fibrosis‐related proteins and inflammation‐related proteins were estimated.TH reduced myocardial infarct area and cardiac enzyme levels, improved cardiomyopathic damage and haemodynamic indexes, and attenuated myocardial fibrosis, the protein expression levels of collagen I and III, myocardial apoptosis, the levels of inflammatory cytokines and inflammation‐related proteins. Notably, the immunofluorescence and protein expression levels of SIRT3 were upregulated in the 34H+DMSO group compared to the I/R group, but this protective effect was abolished by the SIRT3 inhibitor 3‐TYP. After administration of Mcc950, the reversal effects of 3‐TYP were significantly abolished, and TH could protect against MIRI in a rat isolated heart model by inhibiting inflammation and fibrosis. The SIRT3/NLRP3 signalling pathway is one of the most important signalling pathways in this regard.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Therapeutic hypothermia alleviates myocardial ischaemia–reperfusion injury by inhibiting inflammation and fibrosis via the mediation of the SIRT3/NLRP3 signalling pathway

Zhang

et al. 2022

J Cellular Molecular Medi

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“…As for MLZD, various components hold great potential for SIRT3 activation. For example, ginsenoside Rg3, a bioactive ingredient of Panax ginseng, ameliorates mitochondrial dysfunction and apoptosis through the SIRT1/PGC-1α/SIRT3 pathway ( 105 ); long-term consumption of ginseng extract exerted protective effects on intermediate-aged hearts in rats, which might be mediated partly through the upregulation of SIRT3 ( 106 ); Kaempferol could not only increase SIRT3 gene expression but also promoted the expression of deacetylase SIRT3 in the mitochondria ( 107 ); Kaempferol could additionally alleviate H9c2 cardiomyocyte ischemia/reperfusion injury through the activation of SIRT3 to inhibit oxidative stress ( 108 ); In researches investigating the role of luteolin in cerebral ischemia-reperfusion and ultraviolet radiation B-induced photoaging, luteolin has been reported to exert cellular protection by targeting and promoting SIRT3 ( 103 , 109 ). In the current study, 3-TYP, the SIRT3 inhibitor 3-TYP blocks the protective effect of MLZD on cardiomyocytes and mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Modified Linggui Zhugan Decoction protects against ventricular remodeling through ameliorating mitochondrial damage in post-myocardial infarction rats

Xiang

Zhao

et al. 2023

Front. Cardiovasc. Med.

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IntroductionModified Linggui Zhugan Decoction (MLZD) is a Traditional Chinese Medicine prescription developed from Linggui Zhugan Decoction (LZD) that has been used for the clinical treatment of ischemic cardiovascular diseases. However, the cardioprotective mechanism of MLZD against post-myocardial infarction (MI) ventricular remodeling remains unclear.MethodsWe explored the effects of MLZD on ventricular remodeling and their underlying mechanisms, respectively, in SD rats with MI models and in H9c2 cardiomyocytes with oxygen-glucose deprivation (OGD) models. The cardiac structure and function of rats were measured by echocardiography, HE staining, and Masson staining. Apoptosis, inflammation, mitochondrial structure and function, and sirtuin 3 (SIRT3) expression were additionally examined.ResultsMLZD treatment significantly ameliorated cardiac structure and function, and thus reversed ventricular remodeling, compared with the control. Further research showed that MLZD ameliorated mitochondrial structural disruption, protected against mitochondrial dynamics disorder, restored impaired mitochondrial function, inhibited inflammation, and thus inhibited apoptosis. Moreover, the decreased expression level of SIRT3 was enhanced after MLZD treatment. The protective effects of MLZD on SIRT3 and mitochondria, nevertheless, were blocked by 3-TYP, a selective inhibitor of SIRT3.DiscussionThese findings together revealed that MLZD could improve the ventricular remodeling of MI rats by ameliorating mitochondrial damage and its associated apoptosis, which might exert protective effects by targeting SIRT3.

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Cuscuta chinensis flavonoids alleviate ovarian damage in offspring female mice induced by BPA exposure during pregnancy by regulating the central carbon metabolism pathway

Zhao,

Liu,

Chuo

et al. 2024

Ecotoxicology and Environmental Safety

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The Protective Effect of Kaempferol Against Ischemia/Reperfusion Injury Through Activating SIRT3 to Inhibit Oxidative Stress

Cited by 6 publications

References 30 publications

Therapeutic hypothermia alleviates myocardial ischaemia–reperfusion injury by inhibiting inflammation and fibrosis via the mediation of the SIRT3/NLRP3 signalling pathway

Therapeutic hypothermia alleviates myocardial ischaemia–reperfusion injury by inhibiting inflammation and fibrosis via the mediation of the SIRT3/NLRP3 signalling pathway

Modified Linggui Zhugan Decoction protects against ventricular remodeling through ameliorating mitochondrial damage in post-myocardial infarction rats

Cuscuta chinensis flavonoids alleviate ovarian damage in offspring female mice induced by BPA exposure during pregnancy by regulating the central carbon metabolism pathway

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