Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups

Elçik, Deniz; Tunçay, Aydın; Şener, Elif Funda; Taheri, Serpil; Tahtasakal, Reyhan; Mehmetbeyoglu, Ecmel; Güneş, İbrahim; Emiroğullari, Ömer

doi:10.21470/1678-9741-2020-0330

Cited by 5 publications

(11 citation statements)

References 20 publications

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“…Therefore, the occurrence of ventricular fibrillation may be an indicator of poor myocardial protection during surgical procedures. According to the finding from Elcik et al [ 15 ] , at the messenger ribonucleic acid (mRNA) expression level of associated markers, HTK cardioplegia caused more damage to the myocardium compared to blood cardioplegia. In addition, a significant increase in ventricular fibrillation after aortic cross-clamp removal with HTK cardioplegia has been consistently reported [ 4 , 12 ] .…”

Section: Discussionmentioning

confidence: 99%

Comparison of Single-Dose Cardioplegia in Valvular Heart Surgery: Lactated Ringer’s-Based del Nido vs. HistidineTryptophan-Ketoglutarate Cardioplegia Solution

Kantathut,

Luangpatom-aram,

Khajarern

et al. 2023

Braz J Cardiovasc Surg

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Introduction This study evaluated myocardial protection and clinical outcomes when using lactated Ringer’s solution as the base solution for del Nido cardioplegia compared with histidine-tryptophan-ketoglutarate (HTK) solution in valvular surgery. Methods From January 2017 to May 2018, 71 adult patients who underwent valvular surgery with del Nido cardioplegia (n=37) or HTK cardioplegia (n=34) were retrospectively analyzed. Results Patients’ characteristics were comparable between groups. Postoperative peak troponin T levels were similar. The del Nido group had a decreased incidence of ventricular fibrillation after aortic cross-clamp removal (13.51 vs. 55.88%; P <0.001), lower total volume of cardioplegia administered (1,000 [1,000, 1,250] vs. 1,800 [1,500, 2,000] mL; P <0.001), shorter hospital stay (6 [5, 8] vs. 7 [6, 10] days; P =0.03), and less postoperative red cell transfusion (34.29 vs. 61.11%; P =0.024). There is no difference in aortic cross-clamping time, postoperative change in left ventricular ejection fraction, intensive care unit stay, duration of inotropic support, new onset of atrial fibrillation, in-hospital mortality, complications, and three-year overall survival rate. Conclusion Lactated Ringer’s-based del Nido cardioplegia can be safely used for valvular surgery with acceptable clinical outcomes compared to HTK cardioplegia.

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Section: Discussionmentioning

confidence: 99%

Comparison of Single-Dose Cardioplegia in Valvular Heart Surgery: Lactated Ringer’s-Based del Nido vs. HistidineTryptophan-Ketoglutarate Cardioplegia Solution

Kantathut,

Luangpatom-aram,

Khajarern

et al. 2023

Braz J Cardiovasc Surg

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“…18 No total, foram identificados 1.900 procedimentos cirúrgicos cardíacos, dos quais 126 (7%) utilizaram o Custodiol e 1.774 (93%) utilizaram a CS como principal agente cardioplégico no estudo de Viana et al Os resultados mostraram que Custodiol pode ser conveniente e pelo menos tão seguro quanto a CS para proteção miocárdica em cirurgia cardíaca complexa. 1 Elcik et al, 17 mostraram que os resultados do mRNA sanguíneo deram melhores resultados na cardioplegia sanguínea. Nesse estudo, ao contrário do nosso estudo, o fato de os tempos de pinçamento serem diferentes e mais longos também podem motivar a comparação dos dados no sangue.…”

Section: Discussionunclassified

“…Isso pode ser devido aos curtos tempos de clampeamento e à falta de isquemia clara completa no tecido miocárdico. 17 …”

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Comparação de Soluções Cardioplégicas em Cirurgia de Revascularização Miocárdica sobre Mecanismos de Autofagia e Apoptose

Şener¹,

Hamurcu²,

Taheri³

et al. 2023

Arquivos Brasileiros De Cardiologia

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Resumo Fundamento A doença arterial coronariana (DAC) devido à isquemia miocárdica causa perda permanente de tecido cardíaco. Objetivos Nosso objetivo foi demonstrar o possível dano ao miocárdio em nível molecular através dos mecanismos de autofagia e apoptose em pacientes submetidos à cirurgia de revascularização miocárdica. Métodos Um grupo recebeu uma solução de cardioplegia Custodiol e o outro grupo uma solução de cardioplegia sanguínea. Duas amostras miocárdicas foram coletadas de cada paciente durante a operação, imediatamente antes da parada cardíaca e após a liberação do pinçamento aórtico. Foram avaliadas as expressões de marcadores de autofagia e apoptose. O nível de significância estatística adotado foi de 5%. Resultados A expressão do gene BECLIN foi significativa nos tecidos miocárdicos do grupo CS (p=0,0078). Os níveis de expressão dos genes CASPASE 3, 8 e 9 foram significativamente menores no grupo CC. Os níveis pós-operatórios de TnT foram significativamente diferentes entre os grupos (p=0,0072). As expressões dos genes CASPASE 8 e CASPASE 9 foram semelhantes antes e depois do pinçamento aórtico (p=0,8552, p=0,8891). No grupo CC, os níveis de expressão gênica de CASPASE 3, CASPASE 8 e CASPASE 9 não foram significativamente diferentes em amostras de tecido coletadas após pinçamento aórtico (p=0,7354, p=0,0758, p=0,4128, respectivamente). Conclusões Com nossos achados, acreditamos que as soluções CC e CS não apresentam diferença significativa em termos de proteção miocárdica durante as operações de by-pass.

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“…Combined with the reported increase in caspase-9 activation in diabetic retinas (Oshitari et al, 2008), these results support a common association between diabetes, caspase-9 activation, and increased susceptibility to ischemic cell death across different organ systems. More recently, Elcik et al (Elcik et al, 2021) showed greater CASP9 mRNA induction in blood samples from patients who underwent custodiol cardioplegia compared to blood cardioplegia. In combination with other autophagy, apoptosis and hypoxia markers, CASP9 expression was associated with hypoxic myocardial damage.…”

Section: Caspase-9 In Cardiovascular Pathologiesmentioning

confidence: 99%

Caspase-9: A Multimodal Therapeutic Target With Diverse Cellular Expression in Human Disease

Avrutsky

Troy

2021

Front. Pharmacol.

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Caspase-9, a cysteine-aspartic protease known for its role as an initiator of intrinsic apoptosis, regulates physiological cell death and pathological tissue degeneration. Its nonapoptotic functions, including regulation of cellular differentiation/maturation, innate immunity, mitochondrial homeostasis, and autophagy, reveal a multimodal landscape of caspase-9 functions in health and disease. Recent work has demonstrated that caspase-9 can drive neurovascular injury through nonapoptotic endothelial cell dysfunction. CASP9 polymorphisms have been linked with various cancers, neurological disorders, autoimmune pathologies and lumbar disc disease. Clinical reports suggest alterations in caspase-9 expression, activity or function may be associated with acute and chronic neurodegeneration, retinal neuropathy, slow-channel myasthenic syndrome, lumbar disc disease, cardiomyopathies, atherosclerosis and autoimmune disease. Healthy tissues maintain caspase-9 activity at low basal levels, rendering supraphysiological caspase-9 activation a tractable target for therapeutic interventions. Strategies for selective inhibition of caspase-9 include dominant negative caspase-9 mutants and pharmacological inhibitors derived from the XIAP protein, whose Bir3 domain is an endogenous highly selective caspase-9 inhibitor. However, the mechanistic implications of caspase-9 expression and activation remain indeterminate in many pathologies. By assembling clinical reports of caspase-9 genetics, signaling and cellular localization in human tissues, this review identifies gaps between experimental and clinical studies on caspase-9, and presents opportunities for further investigations to examine the consequences of caspase activity in human disease.

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Blood mRNA Expression Profiles of Autophagy, Apoptosis, and Hypoxia Markers on Blood Cardioplegia and Custodiol Cardioplegia Groups

Cited by 5 publications

References 20 publications

Comparison of Single-Dose Cardioplegia in Valvular Heart Surgery: Lactated Ringer’s-Based del Nido vs. HistidineTryptophan-Ketoglutarate Cardioplegia Solution

Comparison of Single-Dose Cardioplegia in Valvular Heart Surgery: Lactated Ringer’s-Based del Nido vs. HistidineTryptophan-Ketoglutarate Cardioplegia Solution

Comparação de Soluções Cardioplégicas em Cirurgia de Revascularização Miocárdica sobre Mecanismos de Autofagia e Apoptose

Caspase-9: A Multimodal Therapeutic Target With Diverse Cellular Expression in Human Disease

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