The Relationship Between Serum Concentration of Interleukin-35 and FoxP3 Polymorphism in Patients Undergoing Coronary Artery Bypass Graft Surgery

Abstract

Objective To investigate the association between interleukin-35 (IL-35) levels and single nucleotide polymorphisms (rs3761548, rs3761547) of the FoxP3 gene in coronary artery bypass grafting (CABG) patients. Methods We conducted a prospective study including 140 patients, who were scheduled for elective isolated on-pump CABG with cardiopulmonary bypass (CPB) from January 2017 to September 2018 in the Jorjani heart center. Blood samples were collected before and 12 hours after the operation. Serum levels of IL-35 were measured by enzyme-linked immunosorbent assay and the pattern of genetic variations was assessed using single specific primer-polymerase chain reaction. Results The serum concentrations of IL-35 after surgery were significantly higher than pre-surgery levels (18.4±8.3 vs . 9.89±3.2, respectively, P =0.002). There was no significant association between genotype frequencies of rs3761548 and rs3761547 and elevated IL-35 levels ( P >0.05). There were significant associations between IL-35 levels and preoperative variables, including age (r=-0.34, P =0.047) and body mass index (r=-0.41, P =0.045), and intraoperative variables, including CPB time (r=0.4, P =0.02) and mean arterial pressure (r=-0.38, P =0.046), in carriers of the rs3761548 AA genotype. Conclusion Serum IL-35 concentrations were significantly increased in CPB patients, which may contribute to the post-CPB compensatory anti-inflammatory response syndrome. IL-35 increased levels were not influenced by FoxP3 promoter polymorphisms (rs3761548, rs3761547).

RORC gene polymorphism is associated with acute kidney injury following cardiac surgery

“…Although, as the first report in this field, there is a need to be suitably cautious in interpreting, the lack of post‐surgery association between rs9017 genotypes and IL‐17 serum level may attribute to a post‐CPB complex interplay of humoral and cellular components of the immune system 38 . Previous studies reported the concurrence of inflammation and immune‐depression during and after CPB, resulting in extensive cytokine production alteration, including tumor necrosis factor‐α, IL‐6, and IL‐8, and transforming growth factor‐β (TGF‐β) 39–41 . Third, the RORγt expression in cells other than Th17 cells 42 such as type 3 innate lymphoid cells (ILC3s), NKT cells, and Tγδ cells, 42 may justify the protective effect of the rs9017 A allele in CPB‐AKI.…”