Heat Shock Proteins: Protection and Potential Biomarkers for Ischemic Injury of Cardiomyocytes After Surgery

Santos-Junior, Valfredo de Almeida; Lollo, Pablo Christiano Barboza; Cantero, Marcos Antonio; Moura, Carolina Soares; Amaya-Farfán, Jaime; Morato, Priscila Neder

doi:10.21470/1678-9741-2017-0169

Cited by 21 publications

(9 citation statements)

References 71 publications

(158 reference statements)

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“…Heat shock proteins (Hsp) are molecular chaperone proteins that facilitate the synthesis and folding of proteins. They are induced in response to environmental stimuli and stressors [31,32]. Therefore, we evaluated the levels of BV-2-derived exosomes expressing heat shock proteins 70 and 90, alpha (α) and beta (β) (Figure 7A–F).…”

Section: Resultsmentioning

confidence: 99%

Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes

Crenshaw

Kumar

Bell

et al. 2019

Biology

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Exosomes are small extracellular vesicles that have emerged as an important tool for intercellular communication. In the central nervous system, exosomes can mediate glia and neuronal communication. Once released from the donor cell, exosomes can act as discrete vesicles and travel to distant and proximal recipient cells to alter cellular function. Microglia cells secrete exosomes due to stress stimuli of alcohol abuse. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of exosomes derived from microglia cell line BV-2. The BV-2 cells were cultured in exosome-free media and were either mock treated (control) or treated with 50 mM or 100 mM of alcohol for 48 and 72 h. Our results demonstrated that alcohol significantly impacted BV-2 cell morphology, viability, and protein content. Most importantly, our studies revealed that exosome biogenesis and composition was affected by alcohol treatment.

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Section: Resultsmentioning

confidence: 99%

Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes

Crenshaw

Kumar

Bell

et al. 2019

Biology

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“…Hsps are molecular chaperone proteins that mediate the synthesis and folding of proteins. They are induced in response to environmental stimuli and stressors such as alcohol [43,44]. In addition, cancer cells also secrete EVs that carry Hsps, which can alter tumor progression [45][46][47].…”

Section: Alcohol Dosing Increases Heat Shock Proteins Within Evsmentioning

confidence: 99%

Alcohol Exposure Impacts the Composition of HeLa-Derived Extracellular Vesicles

et al. 2019

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Extracellular vesicles are nanosized vesicles that are under intense investigation for their role in intercellular communication. Extracellular vesicles have begun to be examined for their role in disease protection and their role as disease biomarkers and/or vaccine agents. The goal of this study was to investigate the effects of alcohol exposure on the biogenesis and composition of extracellular vesicles derived from the cervical cancer line, HeLa. The HeLa cells were cultured in exosome-free media and were either mock-treated (control) or treated with 50 mM or 100 mM of alcohol for 24 h and 48 h. Our results demonstrated that alcohol significantly impacts HeLa cell viability and exosome biogenesis/composition. Importantly, our studies demonstrate the critical role of alcohol on HeLa cells, as well as HeLa-derived extracellular vesicle biogenesis and composition. Specifically, these results indicate that alcohol alters extracellular vesicles’ packaging of heat shock proteins and apoptotic proteins. Extracellular vesicles serve as communicators for HeLa cells, as well as biomarkers for the initiation and progression of disease.

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“…The other possible influence was the group's sex prevalence. Sex hormone alters the expression of HSP 70 differently in women and men [27] . However, it's unclear if the men are more sensitive to induction of HSP 70 expression by the inhibitory effect of estrogen on the HSP 70 expression [28] or if the women hormone doesn't have this inhibitory effect and other factors are involved in upregulation of HSP 70 expression [29] .…”

Section: Discussionmentioning

confidence: 99%

The Expression of and Preoperative Correlation between Heat-Shock Protein 70, EuroSCORE, and Lactate in Patients undergoing CABG with Cardiopulmonary Bypass

Cantero

Almeida

Morato

et al. 2019

Braz J Cardiovasc Surg

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Objetive Coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) improved symptoms and increased survival and quality of life in patients with coronary artery disease. However, it should be the main cause of a complex organic systemic inflammatory response that greatly contributes to several postoperative adverse effects. Methods We aimed to evaluate heat-shock protein 70 (HSP 70) expression as a morbimortality predictor in patients with preserved ventricular function undergoing coronary artery bypass grafting (CABG) with cardiopulmonary bypass (CPB) and to determine their association with the lactate as a marker of tissue hypoperfusion and the EuroSCORE risk score. This is a prospective, observational study including 46 patients and occurring between May and July 2016. Patients without ventricular dysfunction undergoing myocardial revascularization with extracorporeal circulation were included. They were divided into (1) complicated and (2) uncomplicated postoperative evolution groups. EuroSCORE, lactate levels, and HSP 70 expression and their correlations were determined. Results Statistical analysis showed that the group with complicated evolution had higher EuroSCORE values than the other group. HSP 70 protein levels were significantly increased in the group with uncomplicated evolution and showed similar results. According to our results, HSP family proteins may be independent predictors of uncomplicated evolution in patients without ventricular dysfunction undergoing CABG with CPB. Conclusion HSP 70 should be a good discriminator and protection marker for complications in cardiac surgery.

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Heat Shock Proteins: Protection and Potential Biomarkers for Ischemic Injury of Cardiomyocytes After Surgery

Cited by 21 publications

References 71 publications

Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes

Alcohol Modulates the Biogenesis and Composition of Microglia-Derived Exosomes

Alcohol Exposure Impacts the Composition of HeLa-Derived Extracellular Vesicles

The Expression of and Preoperative Correlation between Heat-Shock Protein 70, EuroSCORE, and Lactate in Patients undergoing CABG with Cardiopulmonary Bypass

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