Change in plasma sirtuin 1 level by injection into uterus of resveratrol in Korean cattle

Abstract

How to cite: Kim DH, Yi JK, Ha JJ, Oh DY, Kim DY, Lee YS. Change in plasma sirtuin 1 level by injection into uterus of resveratrol in korean cattle. AbstractBovine embryonic development is closely associated with mitochondrial biogenesis, which is regulated by the sirtuin 1 (SIRT1). Resveratrol, which is a type of natural phenol produced by several plants and used as a dietary supplement, is the activator of SIRT1. Although it has been reported that resveratrol increased SIRT1 level in in vitro bovine blastocysts, there are no in vivo reports on the change in the plasma SIRT1 level in cows. Therefore, we investigated the change in the level of plasma SIRT1 by injecting different concentrations of resveratrol into the uterus of Korean cattle heifer. The level of plasma SIRT1 in the 1.0 μM resveratrol-injected group was the highest among all groups (P < 0.05). Although the level of plasma SIRT1 increased on days 7, 9, and 14 in the resveratrol-injected group, the level of plasma SIRT1 in the control group decreased. When 1.0 µM resveratrol was directly injected into the uterus of cows during artificial insemination, a pregnancy rate was 21.0% higher than that in the control group. In conclusion, our results identified that the level of plasma SIRT1 was increased by direct injection of resveratrol and improved conception rate by injection into uterus of cow during artificial insemination.

Effect of oestrus synchronisation through ovulation delay by vaccination against foot‐and‐mouth disease in Hanwoo (Bos taurus coreanae) cows

“…The OvSynch is effective when there is a large follicle of >10 mm in the random stage, and it is expected that delaying ovulation through FMD vaccination can lead to a higher oestrous rate. Although not shown in the results, 86.6% (104/120) of cows vaccinated with FMD had delayed ovulation (follicle size >10 mm), which was significantly higher than 69.4% (41/59) of those with large follicles (>10 mm) at the random stage in our previous study (D. H. Kim et al, 2020).…”

“…However, the final goal of these oestrus and ovulation synchronisation methods is to achieve higher conception rate by improving oestrus and ovulation synchronisation rates (Bo et al., 2019 ; D. H. Kim et al., 2020 ; Perez‐Mora et al., 2020 ).…”

The Influence of Resveratrol on Conceiving of Repeat Breeder Cows

“…Pretreatment of blastocysts with 1.0 μM resveratrol has been reported to enhance pregnancy rates via embryo transfer in Japanese land cows (Hayashi et al, 2018). Kim et al (2020) stating that a pregnancy rate was 21.0% higher than that in the control group when 1.0 μM resveratrol was directly injected into the uterus of cows during artificial insemination. However, clinical experiments including resveratrol therapy for infertility treatment have not been made in cows.…”

“…In many studies, resveratrol is discussed as a potentially therapeutic element that prevents infertility due to ovarian aging-by-aging inhibition (Özcan et al, 2015). Injecting resveratrol into the womb of the cow during artificial insemination increases the rate of conception (Kim et al, 2020). However, clinical studies on the use of resveratrol for treatment of repeat breeding have not been performed in cows.…”