Transforming growth factor-beta family members are regulated during induced luteolysis in cattle

Abstract

The transforming growth factors beta (TGFβ) are local factors produced by ovarian cells which, after binding to their receptors, regulate follicular deviation and ovulation. However, their regulation and function during corpus luteum (CL) regression has been poorly investigated. The present study evaluated the mRNA regulation of some TGFβ family ligands and their receptors in the bovine CL during induced luteolysis in vivo. On day 10 of the estrous cycle, cows received an injection of prostaglandin F2α (PGF) and luteal samples were obtained from separate groups of cows (n= 4-5 cows per time-point) at 0, 2, 12, 24 or 48 h after treatment. Since TGF beta family comprises more than 30 ligands, we focused in some candidates genes such as activin receptors (ACVR-1A, -1B, -2A, -2B) AMH, AMHR2, BMPs (BMP-1, -2, -3, -4, -6 and -7), BMP receptors (BMPR-1A, -1B and -2), inhibin subunits (INH-A, -BA, -BB) and betaglycan (TGFBR3). The mRNA levels of BMP4, BMP6 and INHBA were higher at 2 h after PGF administration (P<0.05) in comparison to 0 h. The relative mRNA abundance of BMP1, BMP2, BMP3, BMP4, BMP6, ACVR1B, INHBA and INHBB was upregulated up to 12 h post PGF (P<0.05). On the other hand, TGFBR3 mRNA that codes for a reservoir of ligands that bind to TGF-beta receptors, was lower at 48 h. In conclusion, findings from this study demonstrated that genes encoding several TGFβ family members are expressed in a time-specific manner after PGF administration.

Basic fibroblast growth factor induces proliferation and collagen production by fibroblasts derived from the bovine corpus luteum

“…RNA sequencing indicated that TGFB1 is elevated at 4 and 12 h post PGF2α-induced regression. This is in agreement with previous reports from our laboratory [23,76] and others [77][78][79]. Also in accordance with a previous study [22], we observed that TGFB1 stimulates collagen production by luteal fibroblasts.…”

Effects of neonatal exposure to methoxychlor on corpus luteum in gilts: A transcriptomic analysis

“…Members of the transforming growth factors β superfamily and fibroblast growth factors (FGF) are expressed in the mammalian CL in a time‐specific manner and are involved in luteal function (Haas et al, 2019; Portela et al, 2010). In luteal cells of gilts treated with MXC, the expression of FGF1 ( AFGF ), FGF10 , FGF14 and FGF18 , as well as bone morphogenetic protein receptor type 1B ( BMPR1B ), inhibin subunit α ( INHA ), and inhibin subunit β A2 ( INHBA‐2 ) were upregulated.…”

Dr Association of bone morphogenetic protein 15 and growth differentiation factor 9 with litter size in livestock: a review study

“…The BMPs system in the ovary plays a vital role in regulating granulosa cell proliferation, differentiation, and their responsiveness to follicle stimulating hormone (FSH) (Chu et al, 2018;Sanfins et al, 2018). BMPs in sheep and cattle control granulosa cells proliferation by suppressing the FSH receptors expression (Zhang, Klausen, Zhu, Chang, & Leung, 2015;Haas et al, 2019). During follicle and oocyte maturation in farm animals, BMPs signaling affects animal fertility by regulating sex hormone secretion, gene expression of gonadotropin receptors, and oocyte quality (Figure 1) (Zhang et al, 2015;Sanfins et al, 2018).…”