2023
DOI: 10.1590/s2175-97902023e22540
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Antiproliferative effects of 13α/β-steroids on triple-negative MDA-MB-231 breast cancer cells: unraveling intracellular signaling without ERα

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“…HIF-1α, CA IX, PCNA, ERα, GREB1, cyclin D1, and cleaved PARP expression were evaluated using Cell Signaling Technology (Danvers, MA, USA) antibodies. To analyse HIF-1α, CA IX, and PCNA expression, the samples were sonicated as indicated in [16]. GAPDH antibodies (Cell Signaling Technology, Danvers, MA, USA) were used as a loading control.…”
Section: Immunoblottingmentioning
confidence: 99%
“…HIF-1α, CA IX, PCNA, ERα, GREB1, cyclin D1, and cleaved PARP expression were evaluated using Cell Signaling Technology (Danvers, MA, USA) antibodies. To analyse HIF-1α, CA IX, and PCNA expression, the samples were sonicated as indicated in [16]. GAPDH antibodies (Cell Signaling Technology, Danvers, MA, USA) were used as a loading control.…”
Section: Immunoblottingmentioning
confidence: 99%