Synthesis and characterization of Sophora alopecuroides L. green synthesized of Ag nanoparticles for the antioxidant, antimicrobial and DNA damage prevention activity

Abstract

In this study, it was aimed to investigate the amount of antioxidant, protective properties against DNA damage and antibacterial properties against various pathogens after the interaction of Ag metal (Ag NPs/Sa) of Sophora alopecuroides L. (S. alopecuroides L) plant seed, which is grown in Iğdır and used in the treatment of many diseases. The DPPH radical quenching activity of Ag NPs/Sa was performed by using Blois method, DNA damage prevention activity by gel electrophoresis and antibacterial property by disk diffusion method. With the green synthesis method, AgNPs obtained as a result of the reaction of the plant and Ag metal are UV visible spectrophotometer (UV-vis), fourier-transformed infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and scanning electron microscope (SEM). DPPH radical quenching activity of Ag NPs/Sa was investigated in the concentration range of 25-250 μg/ml. The radical quenching activity at a concentration of 250 μg/ml was 85,215 ± 0,101%, while this value was 93,018% for the positive control BHA. It has been observed that the protective property of pBR322 plasmid DNA damage against OH radicals originating from H 2 O 2 increases with concentration. It has been observed that Ag NPs/Sa has significant antimicrobial properties against some pathogens (B.

Anti-corrosion efficiency of Arum italicum root extracts, green synthesis of silver oxide nanoparticles and their applications for photocatalytic and antimicrobial activities

“…Silver nanoparticles demonstrated efficacy against both gram (+) and gram (-) bacteria, confirming their antimicrobial potential. In addition, a reduction in microbial growth was observed with increasing nanoparticle concentration, as previously reported [35] .These promising results suggest a strong antimicrobial potential of the substance, surpassing the performances obtained in other studies on the antimicrobial potential of Ag NPs synthesised using Arum italicum [36,37].…”

“…a peak was observed at 333.5 in the UV-vis spectrum of the AI plant extract, whereas for Ag NPs/AI, it was observed that the maximum signal completely disappeared at 670 nm and the signal intensity around 406,5 nm was considerably reduced, however in the literature the AgNO3 (Ag +1 ) solution gave two peaks at 670 and 405 nm in the UV-vis spectrum. This can be interpreted as a substantial reduction of the cation Ag +1 in metallic silver [21,22].…”