In vitro cytotoxicity assay of Fucoidan extracted from Turbinaria conoides against cancer cell lines MCF7, A549, and normal cell line L929

Abstract

The main aim of the study is to quantify the cytotoxic property of the Fucoidan extracted from the Turbinaria conoides using the MTT assay with the standard fucose. Fucoidan was extracted using the soaked water method and it was determined using the HPLC procedure the obtained Test sample Fucoidan extracted from the Turbinaria conoides and standard fucose was subjected to the cytotoxicity assay against the MCF7 Human breast cancer cell line, A549 lung cancer cell line, and L929 normal mouse fibroblast cell line. From the results it was found that the Test sample showed good IC50 value for MCF7 cell line then A549 with an increasing concentration 24 hours incubation at 37°C The IC50 for MCF7 was 115.21 µg/ml and A549 396.46µg/ml and the Fucoidan extract was checked for its cytotoxicity against the normal mouse fibroblast cell line L929, Fucoidan was found non-lethal to the L929 mouse fibroblast normal cell line. Standard fucose also gave a significant result towards MCF7 and against the L929. This indicates that the Fucoidan extracted from Tubinaria conoides shows better anticancer potential in it. Hence its application can be further extended in the pharmacological fields.

Cytotoxic Activity of Green Seaweed Halimeda tuna Methanolic Extract Against Lung Cancer Cells

“…Different solvents can affect the yield due to the level of polarity. According to Muzaki et al [30], the yield value decreases along with the decrease in the polarity of the solvent. In addition, the solvent will attract bioactive compounds that have the same polarity.…”

“…The yield is the result of a comparison between the total mass of H. tuna extract in the form of paste with the initial mass of H. tuna in the form of dried seaweed [30]. The yield of H. tuna methanolic extract obtained was 0.17±0.04%.…”

“…Prasetyaningrum et al [28] indicated that the cytotoxicity of a substance based on its IC50 is divided into three levels: potential cytotoxic (IC50 <100 μg/mL), moderate cytotoxic (100 μg/mL < IC50 <1000 μg/mL), and low cytotoxic (IC50 >1000 μg/mL). Furthermore, according to the National Cancer Institute [29], a compound can be classified as a strong anticancer agent if its IC50 is Previous research reported the cytotoxicity of brown seaweed fucoidan extracted from Turbinaria conoides species against A549 with IC50 of 396.46 μg/mL [30].…”

In vitro anticancer potential of laminarin and fucoidan from Brown seaweeds

“…Both laminarin and fucoidan extracted from Laminaria japonica were injected into mice and observed that the fucoidan showed strong activation of the immune system than laminarin and therefore, fucoidan enhanced the anticancer efficacy against Lewis lung carcinoma 35 . Fucoidan from Turbinaria conoides , had shown significant anticancer activity on MCF-7 (human breast cancer), and A549 (human lung cancer) cell lines, and no cytotoxic activity on L929 mouse fibroblast cell line 36 . Similarly, fucoidan from Sargassim cinereum inhibited the proliferation of CaCo-2 cell line (Colon cancer) on a dose-dependent manner 37 .…”