Antioxidative Propolis From Stingless Bees (Heterotrigona Itama) Preserves Endothelium-Dependent Aortic Relaxation of Diabetic Rats: The Role of Nitric Oxide and Cyclic Guanosine Monophosphate

Yeoh, Boon Seng; Omar, Norsuhana; Mohammad, Mahaneem; Mokhtar, Siti Safiah; Ahmad, Rozaziana

doi:10.1590/s2175-97902020000419187

Cited by 2 publications

(5 citation statements)

References 25 publications

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“…Furthermore, it can increase the production of NO and cGMP, so can cause aortic vasodilation and can increase the diameter of the aortic lumen. The same results also occur in the administration of propolis antioxidant from Heterotrigona itama, which can increase the relaxation response of acetylcholine to contraction of phenylephrine in the aorta of diabetic rats and works by increasing the production of NO and cGMP [ 20 ].…”

Section: Discussionmentioning

confidence: 86%

“…In the diabetic rat model, STZ increases ROS, and induces oxidative stress, which plays an important role in endothelial cell dysfunction. Therefore, it has been necessary to investigate several parameters of oxidative stress and endothelial cell dysfunction, such as MDA, SOD, GPx, Nrf2, NO, cGMP, and the relaxation response of Ach in diabetic rat tissue [ 9 , 20 , 21 ]. STZ inhibits the secretion of insulin in the Langerhans islet beta cell, which leads to hyperglycemia complications such as endothelial cell dysfunction.…”

Section: Discussionmentioning

confidence: 99%

“…Our results of this research show that oral administration of fucoidan nanoparticles increased cGMP expression in aortic tissue in diabetic rats. Administering exogenous antioxidants can increase NO formation and cGMP production so that aortic vasodilation can occur [ 20 , 21 ]. The results of this study indicate that the antioxidant activity of fucoidan nanoparticles with a scavenger can inhibit the reaction of superoxide ions with NO so that peroxynitrite which is toxic to endothelial cells is not formed, therefore, fucoidan can increase the formation of NO and cGMP.…”

Section: Discussionmentioning

confidence: 99%

“…Ach can be used to prove the occurrence of endothelial cell dysfunction. In diabetes, there is an inhibition of the Ach relaxation response due to endothelial cell dysfunction [ 19 , 20 ]. The results of this study show that the antioxidant activity of fucoidan nanoparticles increased the ACh relaxation response to norepinephrine contractions in the aortic blood vessels of diabetic rats.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, natural products that have antioxidant effects can be used to prevent endothelial cell damage in diabetes [ 17 , 18 , 19 ]. The antioxidants from natural products such as Cinnamomum cassia and Heterotrigona itama could be used as protection against endothelial cell dysfunction in diabetic complications, as they are shown to decrease ROS and increase the NOS, NO, and cGMP in rat aortic tissue that was given streptozotocin intraperitoneally [ 20 , 21 ]. Fucoidan derived from seaweed is reported to have strong antioxidant activity.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Mechanism of Fucoidan Nanoparticles as Protector on Endothelial Cell Dysfunction in Diabetic Rats’ Aortas

et al. 2023

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Antioxidants have an important role in protecting against diabetes complications such as vascular endothelial cell damage. Fucoidan has strong antioxidant properties, therefore the aim of this study was to investigate the protective mechanism of fucoidan nanoparticles through the pathway of antioxidant activity against streptozotocin-induced diabetic aortic endothelial cell dysfunction in rats. Fucoidan nanoparticles are made utilizing high-energy ball milling. This research consists of five groups, namely: control rats, rats were administered aquadest; diabetic rats, rats were administered streptozotocin (STZ); fucoidan nanoparticle rats, rats were administered STZ and fucoidan nanoparticles. Aortic tissue was collected for the evaluation of ROS (reactive oxygen species), Malondialdehyde (MDA), superoxide Dismutase (SOD), Glutathione Peroxidase (GPx), Nuclear factor erythroid-2-related factor 2 (Nrf2), Nitric Oxide (NO), cyclic Guanosine Monophosphate (cGMP), relaxation response of acetylcholine (Ach), and the diameter of the aorta. The size distribution of the fucoidan nanoparticles was 267.2 ± 42.8 nm. Administration of fucoidan nanoparticles decreased the levels of ROS and MDA, and increased the levels of SOD, levels of GPx, Nrf2 expression, NO levels, cGMP expression, the relaxation response of Ach, and lumen diameter of the aorta, which are significantly different when compared with diabetic rats, p < 0.05. In this study, we concluded that the mechanism pathway of fucoidan nanoparticles prevents aortic endothelial cell dysfunction in diabetic rats through antioxidant activity by reducing ROS and MDA and incrementing SOD levels, GPx levels, and Nrf2 expression. All of these can lead to an elevated relaxation response effect of Ach and an increase in the lumen diameter of the aorta, which indicates a protective effect of fucoidan nanoparticles on aortic endothelial cells.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Mechanism of Fucoidan Nanoparticles as Protector on Endothelial Cell Dysfunction in Diabetic Rats’ Aortas

et al. 2023

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In Vivo Toxicity Study on The Effects of Aqueous Propolis Extract From Malaysian Stingless Bee (Geniotrigona thoracica) in Mice

Muhamad Suhaini,

Pauzi,

Juhari

et al. 2023

MAB

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Geniotrigona thoracica is a stingless bee species of Trigona genus. Propolis resinous is a natural product obtained from a honeybees hive with geographical and floral specifications or exudate as such by-products resulting from a variety of botanical processes. Despite its long use for a variety of health conditions, the toxicity profile of propolis sourced from Malaysian stingless bees has not been sufficiently evaluated. For in vivo toxicity assessment, the acute oral toxicity on the effects of aqueous propolis extracts (APE) was examined. Male mice swiss strain, were subjected to acute toxicity testing for 14 days. The APE at doses of 400, 1000 and 2000 mg/kg body weight was supplemented daily to the mice through oral gavage. The clinical signs of toxicity and general behaviour, body weight, relative organ weight, and histopathology changes were investigated. In vivo study was focused on the acute toxicity testing group consisting of 4 groups including Normal (NS), 400 mg/kg (APE 400), 1000 mg/kg (APE 1000) dan 2000 mg/kg (APE 2000). Regarding the toxicity profile, it is proposed that APE supplementation did not induce any mortality and no visible signs of toxicity. No significant changes in the body and relative organ weight were recorded. All the internal organs of the mice were macroscopically healthy with no gross lesion. Likewise, histopathological examinations of the kidney showed mild to moderate histological lesions. Interestingly, the lesion was adverse with an increased dosage of the extract supplementation. This study proposed APE has considerable anti-inflammatory activities. It also demonstrated that the propolis extract is relatively safe to be consumed orally at a dose of 2000 mg/kg body weight.

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Antioxidative Propolis From Stingless Bees (Heterotrigona Itama) Preserves Endothelium-Dependent Aortic Relaxation of Diabetic Rats: The Role of Nitric Oxide and Cyclic Guanosine Monophosphate

Cited by 2 publications

References 25 publications

Molecular Mechanism of Fucoidan Nanoparticles as Protector on Endothelial Cell Dysfunction in Diabetic Rats’ Aortas

Molecular Mechanism of Fucoidan Nanoparticles as Protector on Endothelial Cell Dysfunction in Diabetic Rats’ Aortas

In Vivo Toxicity Study on The Effects of Aqueous Propolis Extract From Malaysian Stingless Bee (Geniotrigona thoracica) in Mice

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