Preparation and characterization of liposomes loaded with silver nanoparticles obtained by green synthesis

Espinoza, Joel Toribio; Novak, Robson Schimandeiro; Magalhães, Cássia Gonçalves; Budel, Jane Manfron; Justus, Barbara; Gonçalves, Melissa Marques; Boscardin, Patrícia Mathias Doll; Farago, Paulo Vítor; Paula, Josiane de Fátima Padiha De

doi:10.1590/s2175-97902020000118601

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…This reveals that both acids were repeatedly retained in UEERO-CsNPs3 and UEERO-CsNPs5, stating very good resolution for both acids [70]. Consequently, the total LE of UEERO particles on CsNPs5 and CsNPs3 calculated according to Espinoza et al [42] using UV-vis spectrophotometer was about 80.05 and 64.39%, respectively that may be due to the enhanced positive charge of NPs.…”

Section: Discussionmentioning

confidence: 78%

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In Vitro Study to Evaluate the Efficacy of Ultrasonicated Ethanolic Extract of Rosmarinus officinalis and its Chitosan-Based Nanoparticles Against Eimeria tenella Oocysts of Chickens

et al. 2022

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In this study, chitosan nanoparticles (CsNPs) were used as nanocarrier for ultrasonicated ethanolic extract of Rosmarinus officinalis (UEERO) as a new nanoformulation against Eimeria tenella. Herein, CsNPs have been synthesized by ionic gelation method at pH 3 (CsNPs3) and pH 5 (CsNPs5), followed by characterization of morphology, size, polydispersity index (PDI), surface charge, and loading efficiency of UEERO. An in vitro sporulation inhibition assay (10, 5, 2.5, 1.25, 0.62, 0.31, 0.15, 0.07, 0.04, 0.02, and 0.01 mg/ml normal saline solution) against E. tenella was conducted. Results showed that free CsNPs and UEERO-CsNPs3/5 were cubic- and spherical-shaped with positive charge and average size of ~ 150.8 nm (314.4 nm) and 151.7 nm (321.1 nm), respectively. The total loading efficiency using UV–vis spectrophotometer, was 80.05 at pH 5 and 64.39% at pH 3. The in vitro sporulation inhibition assay revealed that UEERO, CsNPs3/5, and UEERO-CsNPs3/5 showed a potential inhibitory effect on sporulation (%), distortion in wall (%), and sporocyst abnormality (%) in a dose-dependent manner. Accordingly, the concentration (10 mg/ml) showed the best efficacy after 24 h in UEERO, free CsNPs, and UEERO-CsNPs. Moreover, UEERO-CsNPs3 and UEERO-CsNPs5 had stopped the sporulation (%) after 72 h. Taken all together, UEERO-CsNPs3 and UEERO-CsNPs5 are best effective against E. tenella in a dose-dependent manner in terms of sporulation (%), distortion in wall (%), and sporocysts abnormality. Graphical abstract

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Section: Discussionmentioning

confidence: 78%

“…The absorbance can be calculated by UV-vis spectrophotometer at a wavelength of λmax = 280 nm, and so, the concentrations were detected from the standard curve of the known concentrations from the extract. Finally, the total loading efficiency (LE) of UEERO on CsNPs was calculated according to Espinoza et al [42] by the following equation:…”

Section: Total Lementioning

confidence: 99%