Extract of Solidago chilensis Meyen inflorescences: cytotoxicity and inhibitory activity on nitric oxide synthesis in activated macrophage cell line J774A.1

Brito, Thais Morais de; Amendoeira, Fábio Coelho; Oliveira, Temistocles Barroso de; Frutuoso, Valber da Silva; Ferraris, F; Valverde, Simone Sacramento

doi:10.1590/s2175-97902020000117707

Cited by 2 publications

(6 citation statements)

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“…Fractions in the NO Production and COX-2 Expression In Vitro. We previously demonstrated that the 10 μg/mL concentration of the ScIE significantly reduced nitric oxide (NO) production in the LPS-stimulated J774.A1 cell line [13]. Therefore, we indirectly quantify the NO production in LPS-stimulated J744A.1 cells previously treated with ScHexF, ScDicF, and ScAquF (10 μg/mL) (Figure 4(a)).…”

Section: Evaluation Of the Inhibitory Capacity Of S Chilensismentioning

confidence: 99%

“…After 44 hours, 10% Alamar Blue was added to the wells. After 4 hours of incubation, the fluorescence signal was monitored in a microplate reader (Molecular Devices), Ex: 530-560 nm; Em: 590 nm [13].…”

Section: Characterization and Identification Of Ether-ethanolicmentioning

confidence: 99%

“…Absorbance was read at 540 nm using a microplate reader (Molecular Devices). The concentration of nitrite was calculated from a sodium nitrite standard curve (range 6.5-100 μM) [13].…”

Section: Characterization and Identification Of Ether-ethanolicmentioning

confidence: 99%

“…In experimental models of inflammation, the modulation of these extracts in the inhibition of different inflammatory events, such as edema formation, mast cell degranulation, leukocyte recruitment, and inflammatory mediators' production (e.g., nitric oxide, interleukin-1β, and tumor necrosis factor-α), was observed [10][11][12]. These activities are related to the presence of flavonoid quercetrin, caffeoylquinic acid derivates, and flavonoid rutin [11,12]; additionally, our recent investigation points to the presence of flavonoids derived from quercetin and kaempferol; and labdane diterpenes are partly responsible for these biological activities [13].…”

Section: Introductionmentioning

confidence: 99%

“…S. chilensis is part of the Brazilian National List of Medicinal Plants of Interest to Unified National Health System, and extracts obtained from rhizomes and inflorescences have been produced for anti-inflammatory use [13]. Although the activity of S. chilensis is evidenced in the literature, there are few studies with enriched/characterized fractions or isolated compounds, especially concerning extracts obtained from the inflorescences, relating them to the anti-inflammatory and antinociceptive activities already well described in this plant species [9,14].…”

Section: Introductionmentioning

confidence: 99%

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Anti-Inflammatory Activity and Chemical Analysis of Different Fractions from Solidago chilensis Inflorescence

Brito

Amendoeira

Oliveira

et al. 2021

Oxidative Medicine and Cellular Longevity

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Solidago chilensis Meyen (Compositae) is a species native to South America (Brazil) popularly known as arnica. In Brazilian popular medicine, inflorescences and rhizomes of this plant have been used since the end of the 19th century to replace the exogenous and hepatotoxic Arnica montana L. in the treatment of edema and inflammatory pathologies. Although the anti-inflammatory activity of S. chilensis is evidenced in the literature, there is a lack of studies with enriched fractions or compounds isolated from it. The objective of the current study was to characterize phytochemically and to evaluate the pharmacological action in vivo and in vitro of the crude extract and the different fractions (hexane, dichloromethane, acetal, butanolic, and aqueous) isolated from the inflorescence of S. chilensis. The inflorescence crude extract (ScIE) and fractions were administered by intraperitoneal route to mice at different doses. In an LPS-induced pleurisy model, inhibition of leukocyte influx was observed for the ScIE and all fractions tested, as compared to controls. Dichloromethane (ScDicF), butanolic (ScButF), and aqueous (ScAquF) were selected for further analysis as they showed the best inhibitory effects in leukocyte migration and inflammatory cytokine and chemokine production: TNF-α, CXCL1/KC, CXCL2/MIP-2, and CCL11/eotaxin-1. In LPS-stimulated J774A.1 cell line, ScIE and the ScDicF exhibited an inhibitory effect on nitric oxide (NO) production and downmodulated the COX-2 expression; ScAquF failed to modulate NO production and COX-2 expression. In phytochemical analysis, HPLC-UV-DAD chromatograms of ScDicF and ScAquF showed the main peaks with UV spectrum characteristics of flavonoids; chlorogenic acid and isoquercetin were the most present phytochemicals identified in the ScAquF, and a high number of n-alkanes was found in ScHexF. Our study was the first to address biological effects and correlate them to phytochemically characterized fractions from inflorescences of S. chilensis.

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Section: Evaluation Of the Inhibitory Capacity Of S Chilensismentioning

confidence: 99%

Section: Characterization and Identification Of Ether-ethanolicmentioning

confidence: 99%

“…Absorbance was read at 540 nm using a microplate reader (Molecular Devices). The concentration of nitrite was calculated from a sodium nitrite standard curve (range 6.5-100 μM) [13].…”

Section: Characterization and Identification Of Ether-ethanolicmentioning

confidence: 99%