Antitumor efficacy of EDTA co-treatment with cisplatin in tumor-bearing mice

Abstract

Ethylenediamine tetraacetic acid (EDTA) is used in various medical applications. The aim of this study is to investigate the antitumor efficacy of EDTA alone or with cisplatin (Cis). Fifty male albino mice were used to assess the median lethal dose (LD 50) of EDTA via intraperitoneal (i.p) injection. To determine the antitumor activity, fifty female albino mice were divided into five groups as the following; Group 1 (Gp1) was negative control; (Gp2-5) inoculated i.p with 2×10 6 Ehrlich Ascites Carcinoma (EAC) cells/mouse. After one day, Gp3, Gp4 and Gp5 injected with Cis (2 mg/kg), EDTA (25 mg/kg) and Cis (2 mg/kg)/EDTA (25 mg/kg) for six days, respectively. At day 14, all groups were sacrificed to assess the tumor profile, liver enzymes (alanine transaminases and aspartate transaminases), kidney function (urea and creatinine) and electrolytes (Na + , K + and Ca 2+). The results showed that the i.p LD 50 of EDTA was 250 mg/kg. Treatment with EDTA alone did not show any antitumor activity and did not interfere with the antitumor efficacy of Cis. Biochemical findings revealed that EDTA had mild toxicity on liver and kidneys functions. In summary, EDTA had no antitumor effect and did not alter the Cis efficacy.

Enhancing Antitumor Efficacy of Cisplatin Low Dose by EDTA in Ehrlich Ascetic Carcinoma Bearing Mice

“…We have recently reported that the treatment with EDTA alone did not show any antitumor activities against Ehrlich ascetic carcinoma (EAC) bearing mice. However, EDTA co-treatment with the therapeutic dose of Cis did not alter its antitumor efficacy [6]. Consistent with our findings, treatment with liposomes loaded with EDTA alone did not show any antitumor effects, however, liposomes loaded with EDTA and doxorubicin could significantly reduce drug toxicity without altering the antitumor activity [7].…”

“…Ethylene diamine tetra-acetic acid (EDTA) has some medical and therapeutic uses such as metal chelator in treatment of lead and iron toxicity [6]. The chelation properties of EDTA are also employed to prevent blood coagulation [7], preserve ophthalmic preparations [8] and to decrease the complications of certain types of anemia and blood transfusion [9].…”

“…We have reported previously that the co-treatment with EDTA along with Cis (2 mg/Kg) at early time point did not alter the antitumor efficacy of Cis. We have also reported that EDTA treatment alone did not show any antitumor activity against EACbearing mice [6]. In this study, the percentages (%) of the change in the total body weight (T.B.W) were monitored by weighting animal groups at the beginning (D 0) and at the end of the experiment (D 14).…”

Graphene oxide and its nanocomposites with EDTA or chitosan induce apoptosis in MCF-7 human breast cancer

“…63 Increased caspase-8 and -9 activities further strengthens the nanocomposite induced apoptosis in MCF-7 cells via caspase 8 and caspase 9 dependent manner. According to El-Naggar and El-Said, 64 EDTA alone had no antitumor effect and did not alter the cisplatin anti-tumor efficacy. Furthermore, the application of EDTA in drug delivery systems could signicantly reduce drug toxicity without inuencing antitumor activity, as previously reported by Song et al 65 The effect of GO, GO-EDTA, and GO-CS on VEGFR-2…”

Effect of sub-acute and sub-chronic administration of Vicia faba's cooking medium containing EDTA on hepato-renal functions in albino mice

“…Due to their nutritional importance, people have shifted their consumption to V. faba as a protein source instead of animal proteins. The seed of V. faba beans is very hard, therefore, the time of cooking process of these beans are relatively long (Farajvand et al 2017;El-Naggar et al 2020). For this reason, different additives were used to decrease the time of cooking process.…”