Oleanolic acid from black raisins, Vitis vinifera with antioxidant and antiproliferative potentials on HCT 116 colon cancer cell line

Abstract

Vitis vinifera (black raisin) is commonly used in traditional medicine for the treatment of various ailments.In the present study, anti-oxidative and anti-cancer efficacy of oleanolic acid from ethyl acetate fraction of black raisins was evaluated and oleanolic acid was isolated without using of any chromatographic techniques and subjected to spectral assessment using UV-Vis spectrophotometer, 1 H NMR, 13 C NMR, MS and FT-IR for structural confirmation. Antiproliferative efficacy of oleanolic acid against human colon adenocarcinoma HCT-116 cells was assessed using cell viability assay. The minimum inhibitory concentration (IC 50 ) was determined and found to be 40 µg/mL at 48h incubation. Furthermore, antioxidant property of oleanolic acid was analyzed using DPPH method (IC 50 is 61.5µg/mL) by compared to standard antioxidants ascorbic acid, gallic acid, pyrogallol and butylated hydroxytoluene. Hence, the present study aims to establish the use of oleanolic acid as a potential therapeutic agent against human colon cancer.

Mechanistic investigations of antitumor activity of a Rhodamine B‑oleanolic acid derivative bioconjugate

“…In terms of the effect phytochemicals exhibit in cancer, a study revealed that OA had an anti-proliferative effect while increasing the oxidative stress in various breast cancer cell lines, thus being a pro-oxidant agent (68). Controversially, on HCT 116 colon cancer cell line, OA exhibited anti-proliferative and antioxidant activity (69). The free radical scavenging activity of OA, analyzed through the same DPPH method used in the current work, showed that OA has a significant free radical inhibitory activity compared to the standard antioxidant ascorbic acid (IC 50 =61.5 µg/ml; 132 µM) (69).…”

“…Controversially, on HCT 116 colon cancer cell line, OA exhibited anti-proliferative and antioxidant activity (69). The free radical scavenging activity of OA, analyzed through the same DPPH method used in the current work, showed that OA has a significant free radical inhibitory activity compared to the standard antioxidant ascorbic acid (IC 50 =61.5 µg/ml; 132 µM) (69). This is in line with our findings, according to which RhodOA maintains the free radical scavenging activity exhibited by OA; moreover, RhodOA has a stronger antioxidant effect at 100 nM, comparable to that of the ascorbic acid control solution (RhodOA IC 50 =60.44 nM).…”

Anticancer Potential of A. marmelos Fruit Extract in Human Colon Cancer Cell Lines is Mediated through the Regulation of EMT Signalling Molecules

“…The dose 50 μg/mL caused 74% inhibition. From the study of Kandasamy Sasikumar et al [35], When the concentration of the extract reaches 45 μg/ml, the efficacy of oleanolic acid against HCT-116 cells is significant, with a drop in the percentage of cell viability. When the extract concentration reaches 400 μg/ml, the percentage of cell viability is significant, according to this study.…”

A Clinical Study of Draksha Gritha in the Management of Anaemia in Pregnancy (Garbhini Pandu)

“…Oleanolic acid is a triterpenoid which exists in nature as a free acid or as an aglycone of triterpenoid saponins and it is often ubiquitously found with its isomer, usolic acid [15] The Oleanolicacid is a phytochemical Triterpenoid found in fruit of Draksha. [16] It has got Anti inflammatory, Antihypertensive properties. [17,18] An oleanolic acid derivative, methyl 3-octanoyloxyiminoolean-12-en-28-oate, also showed anti-inflammatory activity demonstrated by its anti-oedemic effects in rats with carrageenan-induced skin inflammation.…”