Ethnopharmacological activity of Hedera nepalensis K. Koch extracts and lupeol against alloxan-induced type I diabetes

Hashmi, Waleed Javed; Ismail, Hammad; Jafri, Laila; Mirza, Bushra

doi:10.1590/s2175-97902019000318406

Cited by 6 publications

(8 citation statements)

References 34 publications

(29 reference statements)

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“…Next, brain tissues were dissected and stored in saline (0.9%). For enzyme estimation, brain tissues were homogenized in ice-cold 50 mM tris buffer at pH 7.4 followed by centrifugation [ 26 ]. The obtained homogenate was used for the estimation of catalase (CAT) [ 27 ], peroxidase (POD), glutathione (GHS) [ 28 ], superoxide dismutase (SOD) [ 29 ], thiobarbituric acid reactive species (TBARS) [ 30 ], and total proteins [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking

Ismail,

Khalid,

Waseem

et al. 2023

PLoS ONE

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Objective Berberis lycium is an indigenous plant of Pakistan that is known for its medicinal properties. In the current study, we investigated the anti-Alzheimer’s effect of berberine isolated from Berberis lycium. Methods Root extract of B. lycium was subjected to acetylcholinesterase inhibition assay and column chromatography for bioassays guided isolation of a compound. The neuroprotective and memory improving effects of isolated compound were evaluated by aluminium chloride induced Alzheimer’s disease rat model, elevated plus maze (EPM) and Morris water maze (MWM) tests., Levels of dopamine and serotonin in rats brains were determined using HPLC. Moreover, western blot and docking were performed to determine interaction between berberine and β-secretase. Results During fractionation, ethyl acetate and methanol (3:7) fraction was collected from solvent mixture of ethyl acetate and methanol. This fraction showed the highest anti-acetylcholinesterase activity and was alkaloid positive. The results of TLC and HPLC analysis indicated the presence of the isolated compound as berberine. Additionally, the confirmation of isolated compound as berberine was carried out using FTIR and NMR analysis. In vivo EPM and MWM tests showed improved memory patterns after berberine treatment in Alzheimer’s disease model. The levels of dopamine, serotonin and activity of antioxidant enzymes were significantly (p<0.05) enhanced in brain tissue homogenates of berberine treated group. This was supported by decreased expression of β-secretase in berberine treated rat brain homogenates and good binding affinity of berberine with β-secretase in docking studies. Binding energies for interaction of β-secretase with berberine and drug Rivastigmine is -7.0 kcal/mol and -5.8 kcal/mol respectively representing the strong interactions. The results of docked complex of secretase with berberine and Rivastigmine was carried out using Gromacs which showed significant stability of complex in terms of RMSD and radius of gyration. Overall, the study presents berberine as a potential drug against Alzheimer’s disease by providing evidence of its effects in improving memory, neurotransmitter levels and reducing β-secretase expression in the Alzheimer’s disease model.

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Section: Methodsmentioning

confidence: 99%

Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking

Ismail,

Khalid,

Waseem

et al. 2023

PLoS ONE

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“…Rats were exposed to different concentrations of plant extract (0, 50, 100 and 150 mg/kg/day) orally for 28 consecutive days. Doses were selected from previous literature where similar doses of H. nepalensis showed non-toxic effects (Hashmi et al, 2020). Stock solution of plant extract was prepared in methanol and further diluted with DMSO, so the final methanol concentration was 0.5%-1% in saline.…”

Section: In Vivo Experimentsmentioning

confidence: 99%

Assessment of antiandrogenic and antispermatogenic activity of Hedera nepalensis in adult male rats

et al. 2021

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The eventual fate of life on the planet is under the pressure of the population explosion, which is a noteworthy reason for pollution and neediness, particularly in the developing and under developed countries (Thanga et al., 2012). Fertility regulation, including contraceptive methods and infertility management, has become the major concern to improve reproductive health (Gupta & Sharma, 2006).Despite the fact that advances have been made for the development of safe, improved and effective contraceptive agent for female, progress on male is still lagged far behind. Currently available methods for male contraception results either unintended pregnancies or lead to complete sterility (El-Kashoury et al., 2009). Hence, development of safe, reversible and biologically active contraceptives for male is necessary and can provide remarkable public and social health benefits (Dehghan et al., 2006).According to the World Health Organization (WHO), about 80% people use plants for their basic health need (Okigbo et al., 2008) and about 70% of the available drugs are derived from natural and botanicals products (Newman & Cragg, 2016). Many plants having antifertility agents have also been reported with antispermatogenic, semen coagulant and spermicidal activities (Gupta & Tandon, 2004;Sharma et al., 2013). In the recent past, research has been done to explore the biologically active components of medicinal plants that interfere with spermatogenic progression or natural process of reproduction (Aladakatti et al., 2010;Jensen, 2002). Hedera, commonly called as "ivy", is a genus of flowering plants belonging to the family Araliaceae which have about 70 genera and

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“…It is widely cultivated as an ornamental vine in eastern Asia, and has long been used as a herbal medicine for the treatment of diabetes, cancer, etc. [1][2][3][4][5]. Also, Hedera nepalensis is a recognized medicinal plant that has been reported to work in antifungal, antimicrobial, antioxidant and antitumor capacities [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Many researchers have paid attention to the phytochemicals obtained from Hedera nepalensis in the relation to their isolation, purification, and characterization [1][2][3][4][5][6][7][8][9][10][11]. Kizu et al isolated various chemical compounds consisting of a complex mixture of saponins from Hedera nepalensis for the first time [11].…”

Section: Introductionmentioning

confidence: 99%

Structural characterization of water-soluble polysaccharides isolated from leaves of Hedera nepalensis

Wang

Liu

et al. 2023

Chem. Biol. Technol. Agric.

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Background Hedera nepalensis is a traditional medicinal plants, and the dried leaves of it are generally used for the cure and treatment of many diseases, also widely known as Chang-Chun-Teng in Chinese. Until now, structural characterization of water-soluble polysaccharides isolated from leaves of Hedera nepalensis have been scarcely studied, even though the chemical compounds derived from it and their biological activities have been widely studied. Methods Water-soluble polysaccharides (WHNP) were isolated from the dried leaves of Hedera nepalensis, and their structural features were investigated. One neutral polysaccharide fraction (WHNP-N) and three major pectin fractions (WHNP-A2b, WHNP-A2c and WHNP-A3b) were obtained from WHNP, respectively. There was no analysis of the neutral fraction (WHNP-N), while the structural characterization of three major pectin fractions (WHNP-A2b, WHNP-A2c and WHNP-A3b) were further studied by monosaccharide composition, HPGPC, NMR and methylation analyses. Results The results indicated that two fractions WHNP-A2b (Mw = 45.8 kDa) and WHNP-A3b (Mw = 58.6 kDa) were mainly composed of rhamnogalacturonan I (RG-I). In WHNP-A2b, RG-I domains primarily substituted with α-L-1,5/1,3,5-arabinan, type II arabinogalactan (AG-II), β-D-1,4-galactan and/or type I arabinogalactan (AG-I) as side chains, while RG-I-type pectin of WHNP-A3b mainly branched with α-L-1,5/1,3,5-arabinan, β-D-1,4-galactan and AG-II side chains. WHNP-A2c (Mw = 12.4 kDa) was primarily comprised of galacturonic acid (GalA, 60.8%), and enzymatic analysis indicated that this fraction mainly consisted of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II) and homogalacturonan (HG) domains with mass ratios of 1.8:1.0:0.6. On the other hand, WHNP-A2c was found to be rich in RG-I domains, which contained α-L-1,5/1,3,5-arabinan, AG-II, β-D-1,4-galactan and/or AG-I as side chains. The HG domains of WHNP-A2c was released in the form of un-esterified and partly methyl-esterified and/or acetyl-esterified oligogalacturonides with a 1 to 7 degree of polymerization after endo-polygalacturonase degradation. Conclusion Our results reveal the structural characteristics of these polysaccharide fractions, which will contribute to elucidating their structure–activity relationships. Graphical Abstract

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Ethnopharmacological activity of Hedera nepalensis K. Koch extracts and lupeol against alloxan-induced type I diabetes

Cited by 6 publications

References 34 publications

Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking

Bioassays guided isolation of berberine from Berberis lycium and its neuroprotective role in aluminium chloride induced rat model of Alzheimer’s disease combined with insilico molecular docking

Assessment of antiandrogenic and antispermatogenic activity of Hedera nepalensis in adult male rats

Structural characterization of water-soluble polysaccharides isolated from leaves of Hedera nepalensis

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