Quantification of chlorogenic acid, rosmarinic acid, and caffeic acid contents in selected Thai medicinal plants using RP-HPLC-DAD

Abstract

The chlorogenic acid, rosmarinic acid, and caffeic acid contents in 100 selected plants were determined using reversed phase high performance liquid chromatography equipped with diode array detector. The optimum condition was 0.2% phosphoric acid in water (solvent A) and methanol (solvent B) as the mobile phase, which was set at 45% B for 20 minutes at a flow rate of 1.2 mL/min. The column temperature was maintained at 30 °C and the detection wavelength was 325 nm. Among 100 selected plants, 39.64% contained all 3 compounds, 40.54% contained 2 compounds, 14.41% contained only 1 compound, and 5.41% could not detect any of the 3 compounds. The highest contents of chlorogenic acid, rosmarinic acid, and caffeic acid were found in Lonicera japonica flowering buds, Melissa officinalis leaves, and Coffea canephora seeds at the concentration of 9.900 ± 0.004, 19.908 ± 0.171, and 1.233 ± 0.003 g/100 g of dried plant, respectively.

Bioactive Compounds and Antioxidant, Antiperoxidative, and Antihemolytic Properties Investigation of Three Apiaceae Species Grown in the Southeast of Morocco

“…p-Coumaric, chlorogenic, caffeic acids, luteolin, and quercetin were the prevailing compounds retained by these extracts. Our findings are in agreement with those reported by Yao et al [ 35 ] and Chaowuttikul et al [ 36 ] who have analyzed eleven Chinese and two Thai celery cultivars and found caffeic, ferulic, and p-coumaric acids, as well as luteolin and chlorogenic acid, respectively. However, Hostetler et al [ 37 ] did not detect quercetin and rutin in the parsley extract while luteolin was found at the same amount.…”

Comparative Study on the Inhibition of Acetylcholinesterase Activity by Hyptis marrubioides, Hyptis pectinata, and Hyptis suaveolens Methanolic Extracts

“…Chaowuttikul et al also identified rosmarinic, caffeic, and chlorogenic acids and hydrocycinnamic acid derivatives in Hs methanolic extracts [13]. In this work, those phenolic acids were quantified in 100 selected Thai plant methanolic extracts using RP-HPLC-DAD [13].…”

Chemophenetic Significance of Anomalocalyx uleanus Metabolites Are Revealed by Dereplication Using Molecular Networking Tools

“…Chlorogenic acid ( 25 ) and its derivatives are present in Euphorbiaceae and could be isolated from some species of Euphorbiaceae, such as Jatropha aethiopica [ 55 ], Euphorbia peplus [ 56 ], Euphorbia hirta , Phyllanthus emblica , Ricinus communis [ 57 ], Sapium insigne [ 58 ], and Croton antisyphiliticus [ 39 ]. Coumarins are relatively common in many Euphorbiaceae genera, such as Pedilanthus [ 59 ], Cnidoscolus [ 22 ], Macaranga [ 60 ], Mallotus [ 61 ], Phyllanthus [ 62 ], Jatropha [ 63 ], Euphorbia [ 64 ], and others.…”

“…The families Euphorbiaceae and Meliaceae belong to the Rosids group; Meliaceae is included in the order Sapindales, while Euphorbiaceae is included in the order Malpighiales [46]. In addition to Meliaceae, cinchonain Ib (33) has already been identified in several families, such as Elaeagnaceae [47], Hypericaceae [48], Lauraceae [49], Rhizophoraceae [50], Rosaceae [51], Rubiaceae [52], Smilacaceae [53], and Theaceae [54] Chlorogenic acid (25) and its derivatives are present in Euphorbiaceae and could be isolated from some species of Euphorbiaceae, such as Jatropha aethiopica [55], Euphorbia peplus [56], Euphorbia hirta, Phyllanthus emblica, Ricinus communis [57], Sapium insigne [58], and Croton antisyphiliticus [39]. Coumarins are relatively common in many Euphorbiaceae genera, such as Pedilanthus [59], Cnidoscolus [22], Macaranga [60], Mallotus [61], Phyllanthus [62], Jatropha [63], Euphorbia [64], and others.…”