Chemical composition and bioactivity of methanolic extract obtained from Lepista sordida

Abstract

The present study aimed at the evaluation of chemical composition and bioactive potential of methanolic extract obtained from Lepista sordida in terms of antioxidative and antimicrobial efficacy. The macrofungus is recognized for its high nutritional value and medicinal properties. However, to the best of our knowledge bioactivity of its methanolic extract is yet to be explored. In this investigation, quantitative analysis of mycochemicals revealed the extract contained significant amount of phenolic compounds such as phenols and flavonoids. Ascorbic acid was found in higher amount than β carotene and lycopene which were present in vestigial amounts. A phenolic profile was also determined using high performance liquid chromatography that further confirmed the presence of 10 phenolic constituents in the extract. Furthermore, the extract was subjected for determining antioxidant potential in different in-vitro assays. The findings showed remarkable 2, 2-Diphenyl-1-picrylhydrazyl and ABTS radical scavenging ability which was evident by low EC 50 values, 330 µg/mL and 30 µg/mL respectively. The extract also demonstrated good chelating and reducing ability, an important marker of antioxidant compounds. Antimicrobial screening displayed positive results against Staphylococcus aureus and Escherichia coli. Altogether, the observations recommend therapeutic application of this mycotaxon on a commercial basis.

A review on Blewit mushrooms ( Lepista sp.) transition from farm to pharm

“…(2.083 ± 1.08 μg/mg) with gallic acid (17.1 ± 0.14 μg/mg) in L. sordida (Table 1) (Acharya et al, 2019). The presence of p-coumaric acid and pro-catechuic acid has also been reported from L. nuda (Table 1) (Ferreira et al, 2009).…”

“…However, β‐carotene and provitamin A are not abundant in L. nuda (Ferreira et al, 2009; Ouzouni et al, 2009). But traces of lycopene (0.045 ± 0.01 μg/mg) and β‐carotene (0.052 ± 0.04 μg/mg) are evidently found in L. sordida (Acharya et al, 2019). The tocopherols, being ample in composition, are analyzed and it is found that the β‐form and γ‐form of the tocopherols are abundant (Reis et al, 2012), whereas the α‐tocopherol isoform is less abundant, and δ‐tocopherol is totally absent in the studied samples (Elmastaş et al, 2007).…”

“…These acids can be of several types as monocarboxylic acid, hydroxyl group bearing carboxylic acid, or carboxylic acids having double bonds. The major quantifiable organic acids found in Lepista species are citric acid (0.2–0.4 g/100 g DW), fumaric acid (0.06–0.07 g/100 g DW), malic acid (1.39–1.79 g/100 g DW), oxalic acid (3.09–3.49 g/100 g DW) (Pinto et al, 2013), quinic acid (2.43–3.89 g/100 g DW), p‐Hydroxybenzoic acid (0.29 g/100 g DW), cinnamic acid (0.08 g/100 g DW) in Lepista nuda (Ferreira et al, 2009; Pinto et al, 2013), and ascorbic acid (2.083 ± 1.08 μg/mg) with gallic acid (17.1 ± 0.14 μg/mg) in L. sordida (Table 1) (Acharya et al, 2019). The presence of p‐coumaric acid and pro‐catechuic acid has also been reported from L. nuda (Table 1) (Ferreira et al, 2009).…”

“…Studies also demonstrated the antimicrobial activity of L. sordida . The methanolic extract of L. sordida exhibits antibacterial activities against gram‐positive bacteria, such as Staphylococcus aureus , Bacillus subtilis , Listeria monocytogenes , and gram‐negative bacteria, Escherichia coli and Salmonella typhimurium , with IC 50 values of 30.6, 535.76, 288.95, 90, and 658.83 μg/ml, respectively (Acharya et al, 2019). Thus, the extract of L. sordida can also be efficiently used in the field of therapeutic research.…”

First identification of potential bioactive compounds from ethanol extracts of Lepista sordida from Indonesia

“…In the past few years, bioactive compounds from mushrooms discovered in Southeast Asia such as Lepista sordida, Cyathus pyrstriatus, Gymnopus sp., Deconia sp., and Lentinus cf. fasciatus were extensively researched due to their potential to have anticancer, antimicrobial, antioxidants, and antitumor properties [8][9][10][11][12].…”