Antidiabetic activity evaluation of Onobrychis species on alloxan-induced diabetic mice

Abstract

In the current study, four Onobrychis species, O. albiflora Hub.-Mor., O. argyrea Boiss. subsp. argyrea Boiss., O. galegifolia Boiss., and O. tournefortii (Willd.) Desv. were collected from Anatolia to be evaluated for their antidiabetic activities. Methanol water extracts of the aerial parts were used for experiments. An alloxan-induced diabetic mice test model was used. Phytochemical analysis of the tested extracts was investigated using the HPLC method. The highest activity was observed with treatment of O. albiflora aerial part extract. Significant decrements were detected in the blood glucose levels as follows: 180.83±47.48 and 252.83±50.47mg/dL at 100 mg/kg and 200 mg/kg doses of O. albiflora, respectively, when compared to the isotonic saline solution control group, eliciting a blood glucose level of 494.20±27.32. Among the tested standard compounds, rutin and isoquercetin were detected in the examined species. The highest amount of rutin (1.1981±0.0017%) and isoquercetin (0.7318±0.0197%) were found in O. albiflora and O. argyrea subsp. argyrea, respectively. Antidiabetic activities of the tested Onobrychis species seem to indicate a possible correlation with their rutin and isoquercetin contents. Therefore, rutin and isoquercetin may be the antidiabetic compounds that contribute to the antidiabetic activity of the tested Onobrychis species.

Antidiabetic Activity of Mung Bean or Vigna radiata (L.) Wilczek Seeds in Alloxan-Induced Diabetic Mice

“…A substance known to cause diabetes, alloxan enters the beta cells of the islets of Langerhans via the GLUT2 transporter, where it inhibits glucokinase and produces reactive oxygen species (ROS) [ 47 , 48 ]. Mice that receive alloxan intraperitoneally at a dose of 150 mg/kg may experience chronic hyperglycemia [ 39 , 48 ]. Similar results were found in our investigation, where the diabetic control group's BGL did not alter significantly during the two-week study.…”

“…Before receiving alloxan, the animals went on a 16-hour fast [35]. Fresh solutions of alloxan were given intraperitoneally to each mouse at a dose of 150 mg/kg after being dissolved with the appropriate volume of normal saline (0.9%) [38][39][40]. Following the administration of alloxan, animals were examined for diabetes 72 hours later, and those with fasting BGL higher than 200 mg/dl were designated as diabetic mice for the study [35,39,41,42].…”

Methanolic Extract of Myrsine africana Leaf Ameliorates Hyperglycemia and Dyslipidemia in Alloxan-Induced Diabetic Albino Mice

“…Before alloxan administration, the mice have been subjected to sixteen hours of fasting. Fresh alloxan solution was prepared using normal saline (0.9%) and administered intraperitoneally at the dose of 150 mg/kg according to previous studies [ 26 – 28 ]. The mice were screened for glucose levels after 72 hours of alloxan administration.…”

“…The mice were screened for glucose levels after 72 hours of alloxan administration. Animals with fasting blood glucose levels >200 mg/dl were included in the study as diabetic mice [ 28 – 30 ]. After the screening, diabetic mice were randomly allocated to the different test groups.…”

Potential use of wild Onobrychis species for climate change mitigation and adaptation

“…Özbek et al. (2019) suggested that methanol water extracts of the aerial parts antidiabetic activities of Onobrychis albiflora Hub‐Mor., Onobrychis argyrea Boiss. subsp.…”