Insulin-loaded polymeric mucoadhesive nanoparticles: development, characterization and cytotoxicity evaluation

Gatti, T; Eloy, Josimar O.; Ferreira, Leonardo Miziara Barboza; Silva, Isabel Cristine da; Pavan, Fernando Rogério; Gremião, Maria Palmira Daflon; Chorilli, Marlus

doi:10.1590/s2175-97902018000117314

Cited by 28 publications

(17 citation statements)

References 41 publications

(56 reference statements)

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“…Gatti et al prepared nanoparticles based on chitosan/dextran sulfate formed by polyelectrolytes condensation for insulin delivery. The encapsulation prevented insulin from partial degradation and displayed sustainable release indicating efficient mucus complexation between mucin and nanoparticles [15].…”

Section: Introductionmentioning

confidence: 99%

Therapeutic efficacy of nanoparticles and routes of administration

et al. 2019

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In modern-day medicine, nanotechnology and nanoparticles are some of the indispensable tools in disease monitoring and therapy. The term “nanomaterials” describes materials with nanoscale dimensions (< 100 nm) and are broadly classified into natural and synthetic nanomaterials. However, “engineered” nanomaterials have received significant attention due to their versatility. Although enormous strides have been made in research and development in the field of nanotechnology, it is often confusing for beginners to make an informed choice regarding the nanocarrier system and its potential applications. Hence, in this review, we have endeavored to briefly explain the most commonly used nanomaterials, their core properties and how surface functionalization would facilitate competent delivery of drugs or therapeutic molecules. Similarly, the suitability of carbon-based nanomaterials like CNT and QD has been discussed for targeted drug delivery and siRNA therapy. One of the biggest challenges in the formulation of drug delivery systems is fulfilling targeted/specific drug delivery, controlling drug release and preventing opsonization. Thus, a different mechanism of drug targeting, the role of suitable drug-laden nanocarrier fabrication and methods to augment drug solubility and bioavailability are discussed. Additionally, different routes of nanocarrier administration are discussed to provide greater understanding of the biological and other barriers and their impact on drug transport. The overall aim of this article is to facilitate straightforward perception of nanocarrier design, routes of various nanoparticle administration and the challenges associated with each drug delivery method.

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Section: Introductionmentioning

confidence: 99%

Therapeutic efficacy of nanoparticles and routes of administration

et al. 2019

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“…Chitosan is widely applied for nano-encapsulation due to its physical-chemical characteristics, including the ability to form gels by ionic bonds and its cationic nature, conferring mucoadhesiveness through electrostatic interactions and favoring the interaction of the transported agent with the mucus layer of epithelial surfaces [ 97 , 98 , 99 , 100 ].…”

Section: Brief Overview Of Nano-encapsulated Polyphenols: Nature and mentioning

confidence: 99%

Increasing the Power of Polyphenols through Nanoencapsulation for Adjuvant Therapy against Cardiovascular Diseases

et al. 2021

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Polyphenols play a therapeutic role in vascular diseases, acting in inherent illness-associate conditions such as inflammation, diabetes, dyslipidemia, hypertension, and oxidative stress, as demonstrated by clinical trials and epidemiological surveys. The main polyphenol cardioprotective mechanisms rely on increased nitric oxide, decreased asymmetric dimethylarginine levels, upregulation of genes encoding antioxidant enzymes via the Nrf2-ARE pathway and anti-inflammatory action through the redox-sensitive transcription factor NF-κB and PPAR-γ receptor. However, poor polyphenol bioavailability and extensive metabolization restrict their applicability. Polyphenols carried by nanoparticles circumvent these limitations providing controlled release and better solubility, chemical protection, and target achievement. Nano-encapsulate polyphenols loaded in food grade polymers and lipids appear to be safe, gaining resistance in the enteric route for intestinal absorption, in which the mucoadhesiveness ensures their increased uptake, achieving high systemic levels in non-metabolized forms. Nano-capsules confer a gradual release to these compounds, as well as longer half-lives and cell and whole organism permanence, reinforcing their effectiveness, as demonstrated in pre-clinical trials, enabling their application as an adjuvant therapy against cardiovascular diseases. Polyphenol entrapment in nanoparticles should be encouraged in nutraceutical manufacturing for the fortification of foods and beverages. This study discusses pre-clinical trials evaluating how nano-encapsulate polyphenols following oral administration can aid in cardiovascular performance.

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“…Such a nanoparticle system could be applied for nose-to-brain delivery [49]. Almost 100% of insulin was released from insulin-encapsulating nanoparticle composed of chitosan/dextran sulfate (6:4 ratio, diameter of 320.55 nm) after approximately 12 h in in vitro test using a dialysis membrane [50]. However, at present, few insulin-loaded nanoparticles targeting the olfactory nerve route have been investigated.…”

Section: Intranasal Administration Using Nanoparticlesmentioning

confidence: 99%

Shortcut Approaches to Substance Delivery into the Brain Based on Intranasal Administration Using Nanodelivery Strategies for Insulin

Tashima

2020

Molecules

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The direct delivery of central nervous system (CNS) drugs into the brain after administration is an ideal concept due to its effectiveness and non-toxicity. However, the blood–brain barrier (BBB) prevents drugs from penetrating the capillary endothelial cells, blocking their entry into the brain. Thus, alternative approaches must be developed. The nasal cavity directly leads from the olfactory epithelium to the brain through the cribriform plate of the skull bone. Nose-to-brain drug delivery could solve the BBB-related repulsion problem. Recently, it has been revealed that insulin improved Alzheimer’s disease (AD)-related dementia. Several ongoing AD clinical trials investigate the use of intranasal insulin delivery. Related to the real trajectory, intranasal labeled-insulins demonstrated distribution into the brain not only along the olfactory nerve but also the trigeminal nerve. Nonetheless, intranasally administered insulin was delivered into the brain. Therefore, insulin conjugates with covalent or non-covalent cargos, such as AD or other CNS drugs, could potentially contribute to a promising strategy to cure CNS-related diseases. In this review, I will introduce the CNS drug delivery approach into the brain using nanodelivery strategies for insulin through transcellular routes based on receptor-mediated transcytosis or through paracellular routes based on escaping the tight junction at the olfactory epithelium.

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Insulin-loaded polymeric mucoadhesive nanoparticles: development, characterization and cytotoxicity evaluation

Cited by 28 publications

References 41 publications

Therapeutic efficacy of nanoparticles and routes of administration

Therapeutic efficacy of nanoparticles and routes of administration

Increasing the Power of Polyphenols through Nanoencapsulation for Adjuvant Therapy against Cardiovascular Diseases

Shortcut Approaches to Substance Delivery into the Brain Based on Intranasal Administration Using Nanodelivery Strategies for Insulin

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