To analyze the effect of mixed liquor administration on the Johnsen's score and the number of Sertoli cells and Leydig cells on the Wistar strain white rats (Rattus norvegicus). A total of 28 rats were divided into 4 groups: C, P1, P2, and P3. Rats were given mixed liquor with different dose, namely P1 (1 ml), P2 (2 ml), and P3 (4 ml) each day for 14 days, then compared to C group which was given 4 ml of distilled water using an oral gavage. Mixed liquor consists of 20% ethanol and 4% methanol. The histopathological features were evaluated by the Johnsen's score, the number of Sertoli cells, and Leydig cells in cross-sectional preparation of rat testicular tissue with 400× magnification. Datas were analyzed using the Kruskal-Wallis test and One-Way ANOVA test with a confidence level of p <0.05. The P3 group had the lowest Johnsen's score and the number of Sertoli cells, 6.442±0.293 and 5.942±0.674, respectively. A significant decrease in increased dose occurred in the Sertoli cell count but not in the Johnsen's score. Group P2 had the lowest number of Leydig cells, 6.421±0.360. The administration of mixed liquor caused a decrease in Johnsen's score and the number of Sertoli cells and Leydig cells on the Wistar strain white rat (Rattus norvegicus).