Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours

Filho, Tomáz de Jesus Maria Grezzana; Longo, Larisse; Santos, Jorge Luiz dos; Gabiatti, Gémerson; Boffil, Carlos; Santos, Emanuel Bendo Dos; Cerski, Carlos Thadeu Schmidt; Chedid, Marcio; Corso, Carlos Otávio

doi:10.1590/s0102-865020200020000005

Cited by 3 publications

(3 citation statements)

References 52 publications

(65 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most of the data devoted to mild hypothermia's effectiveness for stroke treatment is limited to animal studies. Experiments on mice and rats noted its neuroprotective effect 30–32 . However, its mechanisms are still not sufficiently understood.…”

Section: Discussion/conclusionmentioning

confidence: 99%

See 1 more Smart Citation

Role of PERK‐mediated pathway in the effect of mild hypothermia after cerebral ischaemia/reperfusion

Zhao

Xia

Tang

et al. 2023

Eur J Clin Investigation

View full text Add to dashboard Cite

BackgroundHypothermia is an effective method of reducing brain injury caused by a variety of neurological insults. It is aimed to elucidate whether a change in the expression of PERK‐mediated pathway proteins is an indicator of the neuroprotective effect of mild hypothermia after cerebral ischaemia/reperfusion.MethodsOne hundred and ninety‐two male C57BL/6 mice were randomly divided into three groups: a sham group, a cerebral normothermic ischaemia/reperfusion (I/R) group and a cerebral hypothermic I/R group. A cerebral ischaemia model was established by ligating the bilateral common carotid artery for 15 min. Mice in the hypothermia group stayed in a cage that was set at 33°C, sprayed with a spray of 70% ethanol, and blown with two high‐speed fans. The state of neurons was assessed on micropreparations stained with haematoxylin–eosin and TUNEL. The expressions of GRP78, p‐perk, p‐eif2α, ATF4 and CHOP were measured by western blot analysis 6, 12, 24 and 72 h after reperfusion.ResultsThe number of surviving cells was significantly higher in the hypothermia group than in the group without hypothermia (p < .05). The GRP78 expression in the hypothermia group was statistically higher (p < .05) than in the ischaemia/reperfusion group. Optical densities of p‐perk, p‐eif2α and ATF4 in hippocampus CA1 neurons ischaemia were statistically significantly lower in the hypothermia group than in the ischaemia/reperfusion group (p < .05). The CHOP expression in the hypothermia group was statistically lower (p < .05) than in the ischaemia/reperfusion group.ConclusionMild hypothermia for 6 h promoted moderate neuroprotection by mediating the expression of GRP78, p‐PERK, p‐eIF2α, ATF4 and CHOP.

show abstract

Section: Discussion/conclusionmentioning

confidence: 99%

“…Experiments on mice and rats noted its neuroprotective effect. [30][31][32] However, its mechanisms are still not sufficiently understood. This study focused on the neuroprotective mechanism of mild hypothermia.…”

Section: The Expression Of Grp78 P-perk P-eif2α Atf4 and Chopmentioning

confidence: 99%

Role of PERK‐mediated pathway in the effect of mild hypothermia after cerebral ischaemia/reperfusion

Zhao

Xia

Tang

et al. 2023

Eur J Clin Investigation

View full text Add to dashboard Cite

show abstract

“…Reducing the testicular torsion enhances damages due to the ischaemic process, likely as a result of the enhanced vascular permeability (reperfusion injury) (Long et al., 2018; Payabvash et al., 2008; Turner et al., 2005). Researchers presented a lot of treatment options for preserving testicular viability before and following the reduced torsion such as calcium channel blocker, hypothermia, removal of the free radicals, suppression of the free radical formation, mitigation of lipid peroxidation and neutrophilic inhibition (Dokmeci et al., 2007; Grezzana Filho et al., 2020; Solmazgul et al., 2007; Ünsal et al., 2006). However, early surgical intervention accompanied by the reduced torsion has been proposed to be the prioritised procedure to preserve the viability of these testes, but early operations are constantly impossible due to diverse technical reasons.…”

Section: Introductionmentioning

confidence: 99%

Effects of hypothermia and pentoxifylline on the adnexal torsion/detorsion injuries in a rat testis model

et al. 2021

View full text Add to dashboard Cite

This study was designed to investigate the effects of separate and combined administration of hypothermia and pentoxifylline to preserve the effects on the testicles in an experimental model of testicular torsion/ detorsion injuries in rats. Forty male adult Wistar rats were randomly divided into five groups, control, torsion/detorsion (TD), torsion/detorsion/hypothermia (TD+ICE), torsion/detorsion received of pentoxifylline (40mg/kg, ip) (TD+PTX) and torsion/detorsion/hypothermia/PTX (TD+ICE+PTX). Left testicular torsion (TT) was performed for 4 and half hours, and ice fragments have been used at the beginning of torsion. After the reperfusion period (a week), oxidative maker's serum levels, testosterone hormone, sperm parameters, and histopathological and gene expression evaluations have been performed. Significant adverse changes were observed in the TD group for histological variables, sperm count, oxidative marker, testosterone hormone, Bax, BCL2 and caspase‐3 expression. The parameters studied in the group receiving PTX improved in comparison with the TD group, while macroscopical parameters of both the hypothermia and PTX+ICE groups were not different compared with the TD group. The results revealed that PTX, as an antioxidant component, was protective against testicular torsion, while hypothermia and hypothermia plus PTX did not exhibit this property, which may have been due to the duration of hypothermia (4 hr) or reperfusion period.

show abstract

Effects of iNOS in Hepatic Warm Ischaemia and Reperfusion Models in Mice and Rats: A Systematic Review and Meta-Analysis

Nakatake

Schulz

Kalvelage

et al. 2022

IJMS

View full text Add to dashboard Cite

Warm ischaemia is usually induced by the Pringle manoeuver (PM) during hepatectomy. Currently, there is no widely accepted standard protocol to minimise ischaemia-related injury, so reducing ischaemia-reperfusion damage is an active area of research. This systematic review and meta-analysis focused on inducible nitric oxide synthase (iNOS) as an early inflammatory response to hepatic ischaemia reperfusion injury (HIRI) in mouse- and rat-liver models. A systematic search of studies was performed within three databases. Studies meeting the inclusion criteria were subjected to qualitative and quantitative synthesis of results. We performed a meta-analysis of studies grouped by different HIRI models and ischaemia times. Additionally, we investigated a possible correlation of endothelial nitric oxide synthase (eNOS) and nitric oxide (NO) regulation with iNOS expression. Of 124 included studies, 49 were eligible for the meta-analysis, revealing that iNOS was upregulated in almost all HIRIs. We were able to show an increase of iNOS regardless of ischemia or reperfusion time. Additionally, we found no direct associations of eNOS or NO with iNOS. A sex gap of primarily male experimental animals used was observed, leading to a higher risk of outcomes not being translatable to humans of all sexes.

show abstract

Induction of selective liver hypothermia prevents significant ischemia/reperfusion injury in Wistar rats after 24 hours

Abstract: Conception and design of the study, technical procedures, manuscript writing.

Cited by 3 publications

References 52 publications

Role of PERK‐mediated pathway in the effect of mild hypothermia after cerebral ischaemia/reperfusion

Role of PERK‐mediated pathway in the effect of mild hypothermia after cerebral ischaemia/reperfusion

Effects of hypothermia and pentoxifylline on the adnexal torsion/detorsion injuries in a rat testis model

Effects of iNOS in Hepatic Warm Ischaemia and Reperfusion Models in Mice and Rats: A Systematic Review and Meta-Analysis

Contact Info

Product

Resources

About