Therapeutic effect of adenosine on experimentally induced acute ulcerative colitis model in rats

Abstract

Purpose: To examine the therapeutic effect of external adenosine on an acetic acid-induced acute ulcerative colitis model in rats. Methods:Thirty male mature rats were divided into three groups as control, acute colitis (AC) and AC+adenosine group (AC+AD). AC was induced by rectal administration of 4% acetic acid (AA). 5mg/kg/day adenosine was performed i.p for 4 weeks to AC+AD group. Rectum and colon were excised for microscopic and histopathological histopathologic evaluations, and immunohistochemical analysis of nuclear factor kappa B (NF-kB). Blood samples were collected for biochemical detection of TNF-α, Pentraxin-3 and malondialdehyde (MDA) levels.Results: AC group had generalized hyperemia and hemorrhage with increased macroscopic and histopathological scores compared with control (P <0.0001) while adenosine treatment decreased these scores significantly (P <0.001), with reduced distribution of disrupted epithelium, leukocyte infiltrates, and focal hemorrhage. AC group showed significantly increased immunoexpression of NF-kB in rectum, plasma and tissue levels of TNF-α, plasma Pentraxin-3 and MDA levels (P <0.0001) while adenosine reduced these levels (P < 0.05).Conclusion: Adenosine appears to promote healing of colon and rectum exposed to AA-induced AC, suggesting a boosting effect of adenosine on the intestinal immune system to cure ulcerative colitis.

Ban-Lan-Gen Granule Alleviates Dextran Sulfate Sodium-Induced Chronic Relapsing Colitis in Mice via Regulating Gut Microbiota and Restoring Gut SCFA Derived-GLP-1 Production

“…11,12 Previous studies have well documented that the compounds adenosine, uridine, and indirubin exhibit potent anti-colitis effects in different animal colitis models. [13][14][15][16][17] However, no evidence-based study has been conducted to assess the efficacy of BLG against colitis. In the present study, we investigated the protective effect of BLG on dextran sulfate sodium (DSS)-induced chronic relapsing colitis in C57BL/6 mice and found that oral administration of BLG significantly alleviated DSS-induced chronic relapsing colitis in mice, and its regulatory mechanism was associated with regulating gut microbiota and restoring gut derived-glucagon-like peptide-1 (GLP-1) production.…”

Treatment of colitis by oral negatively charged nanostructured curcumin in rats

“…Some other experimental studies have been published using plant extracts and drugs for the treatment of UC, emphasizing other mechanisms as inhibiting the secretion of IL-13, increased immunoexpression of NF-kB in rectum, etc. 38 , 39 . The histopathologic and macroscopic validations of the scores used in the present study were based on previous methods 40 .…”

Anti-inflammatory, anti-apoptotic, and antioxidant effects of obestatin on the colonic mucosa following acetic acid–induced colitis

“…The grading of colonic damage (ulceration, inflammation, depth of the lesion, and fibrosis) and the number of goblet cells were determined using a scale of Vilaseca as described in detail previously [37,54]. The standard techniques of staining of Caspase-3, PCNA, and NFkB were followed as provided by the instructors [1,18,59].…”