The effect of Allium sativum in experimental peritoneal adhesion model in rats

Abstract

Purpose:To evaluate the effect of garlic on formation of postoperative adhesions in rats.Methods:Twenty-four Sprague dawley rats were divided into three groups. In Group 1 (sham), laparotomy was performed and stitched up. In Group 2 (control), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and 2 cc of saline was intraperitoneally administered to each rat. In Group 3 (experimental), after laparotomy was performed, punctate hemorrhage was induced by cecal abrasion in the cecum and each rat was intraperitoneally administered a sterile Allium sativum derivative. The rats in all groups were re-laparotomized on postoperative day 7; samples were obtained from the peritoneal tissue surrounding the cecumResults:In Group 3, there was a statistically significant difference in terms of inflammation, lymph node size, and free oxygen radicals; these parameters tended to increase. In terms of fibrosis evaluated using H&E and MT, there was no significant difference between groups 2 and 3.Conclusions:No positive outcomes indicating that Allium sativum reduces intra-abdominal adhesions were obtained. However, it caused severe inflammation in the tissue. Additionally, in immunohistochemical analyses conducted to detect oxidative stress, allium sativum increased the production of free oxygen radicals in the tissue.

Sıçanlarda Postoperatif Adezyonların Önlenmesinde Masere Sarımsak (Allium Sativum L.) Yağı Kullanımı

“…Because of the uncertainties in products, it was clearly emphasized that it is important to reveal the sulfurous compounds and their amounts in a commercially available garlic product before the use for potential beneficial effects on health [14]. From this aspect, it is thought that the difference between the results reported in previous studies examining the adhesionprevention effect of macerated garlic oil [3,4,7] arose from the use of commercial macerated oils manufactured by different companies and have different chemical compositions. This thought is also corroborated by the fact that oils used in those studies were not analyzed for suitability.…”

“…Intra-abdominal adhesion is a pathological condition, which is observed after various surgical interventions, has a high incidence [1][2][3][4][5], and can cause various complications including stomachache, intestinal obstruction, and female infertility [5,6]. The incidence rate of postoperative adhesions, which are known to occurs on the 3 rd and 5 th postoperative days, after abdominal surgery varies between 55 and 94 % [1].…”

“…In a prospective study examining 1000 patients who have undergone laparoscopic surgery at least once, adhesion incidence was reported to be 21.1% (n=211) and it was emphasized that the adhesions found in intestines in 28% (n=59) of cases [2]. Various agents and methods were analyzed regarding the prevention of adhesion and/or reducing its incidence [1,3,4,[6][7][8][9]. In a study using commercial garlic oil, authors reported no positive result [4], whereas several studies showed that garlic oil was successful in preventing adhesion at certain rates [3,7].…”

“…Various agents and methods were analyzed regarding the prevention of adhesion and/or reducing its incidence [1,3,4,[6][7][8][9]. In a study using commercial garlic oil, authors reported no positive result [4], whereas several studies showed that garlic oil was successful in preventing adhesion at certain rates [3,7].…”

“…The antiseptic properties of garlic originates from allicin (di-allyl-thiosulfate), one of the sulfurous essentail oil [11]. Thanks to its organosulfur components [di-allyl sulfide, di-allyl disulfide (DADS), di-allyl trisulfide (DATS), and di-allyl tetrasulfide], garlic was reported to be protective against various diseases [3,4,7,10,11,13] and to have hypolipidemic, antihypertensive, antioxidant [10][11][12][13][14], anticarcinogenic [12][13][14][15], antimicrobial, antithrombotic, fibrinolytic, and wound-healing effects [3,4,7,10,11,13]. It is recommended to use essential oils obtained from fresh garlic as anticarcinogenic and their aqueous solutions as herbal medication [15].…”