Effect of low power laser in biomodulation of cultured osteoblastic cells of Wistar rats

Abstract

Purpose To analyze aspects of the biomodulating effect of light in biological tissues, bone cells from surgical explants of the femur of rats were irradiated with low intensity laser. Methods Bone cells were cultured and irradiated with LASER light (GaAlAs). Growth, cell viability, mineralized matrix formation, total protein dosage, immunostimulatory properties, cytochemical analysis, gene expression of bone proteins were examined using live cell imaging and cell counting by colorimetric assay. The gene expression of: alkaline phosphatase (ALP), type 1 collagen, osteocalcin and osteopontin through the real-time polymerase chain reaction. Results At 8 days, the viability of the irradiated culture was 82.3% and 72.4% in non-irradiated cells. At 18 days, the cellular viability (with laser) was 77.42% and 47.62% without laser. At 8 days, the total protein concentration was 21.622 mg / mol in the irradiated group and 16, 604 mg / mol in the non-irradiated group and at 18 days the concentration was 37.25 mg / mol in the irradiated group and 24, 95 mg / mol in the non-irradiated group. Conclusion The laser interfered in the histochemical reaction, cell viability, matrix mineralization, and maintained the cellular expression of proteins

Interaction between Laser Light and Osteoblasts: Photobiomodulation as a Trend in the Management of Socket Bone Preservation—A Review

“…Similarly, the 800-900 nm wavelength range, which like 900-1000 nm better penetrates tissues than shorter wavelengths, positively [35,[39][40][41][42] or negatively [35,36] affects both the proliferation and viability of pre-osteoblasts after PBM; in some cases, cells are not influenced [43,44]. Pre-osteoblast differentiation is likewise modulated by the 800-900 nm range PBM, by showing an increase in differentiation markers [37,39,41,42,[45][46][47], a decrease [36] or no response [43,44].…”

“…The IGF-induced PI3-kinase-Akt signaling cascade is essential for bone morphogenetic protein (BMP) 2, runt-related transcription factor (RUNX) [ 37 ], osteopontin [ 30 , 37 ], osteocalcin [ 36 ], collagen type I [ 29 , 31 , 36 ], and bone sialoprotein [ 27 ] expression. Similarly, the 800–900 nm wavelength range, which like 900–1000 nm better penetrates tissues than shorter wavelengths, positively [ 35 , 39 , 40 , 41 , 42 ] or negatively [ 35 , 36 ] affects both the proliferation and viability of pre-osteoblasts after PBM; in some cases, cells are not influenced [ 43 , 44 ]. Pre-osteoblast differentiation is likewise modulated by the 800–900 nm range PBM, by showing an increase in differentiation markers [ 37 , 39 , 41 , 42 , 45 , 46 , 47 ], a decrease [ 36 ] or no response [ 43 , 44 ].…”

Effect of GaAlAs 940 nm Photobiomodulation on palatal wound healing after free gingival graft surgery: a split mouth randomized controlled clinical trial

“…Depending on the dosage, duration, and the level of radiation to wounds, LLLT regulates biochemical processes through uplifting the anti-oxidant system assigned to reduce tissue damages, boosting mitochondria breathing and producing ATP [ 31 ].…”

An Evaluation of the Effects of Photobiomodulation Therapy on the Peri-Implant Bone Healing of Implants with Different Surfaces: An In Vivo Study

“…Its action modulates the tissue repair process, promotes anti-inflammatory and analgesic effects, stimulates the immune system, allows cell regeneration and favors the processes of angiogenesis, collagen synthesis and osteogenesis [6][7][8]. The therapeutic effects of low-level lasers for the treatment of wounds were first described in 1971 by Mester et al [9], followed by several other researchers [10][11][12] who proved the biostimulation effects of laser therapy.…”