Cardioprotective effect of lipstatin derivative orlistat on normotensive rats submitted to cardiac ischemia and reperfusion

Rodrigues, Francisco Sandro Menezes; Errante, Paolo Ruggero; Ferreira, Regiane Miranda; Tavares, José Roberto; Paula, Leandro Raphael N. de.; Araújo, Evaldo Stanislau Affonso de; Govato, Tânia Carmem Peñaranda; Tikazawa, Eduardo Hiroshi; Reis, Maria do Carmo Maia; Filho, Bráulio Luna; Ferraz, Renato Ribeiro Nogueira; Oliveira-Júnior, Itamar de Souza; Taha, Murched Omar; Caricati‐Neto, Afonso

doi:10.1590/s0102-865020180060000007

Cited by 11 publications

(12 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ECG was monitored and recorded from the beginning of the stabilization period with a commercial acquisition system (AqDados 7.02; Lynx Tecnologia Ltda., Brazil) as reported earlier [9][10][11][12][13] . To assess heart rate, incidences of reperfusion-induced ventricular arrythmia (VA), atrioventricular block (AVB) and lethality (LET), raw data were analyzed with a commercial software package included in the acquisition system (AqDAnalysis 7, Lynx Tecnologia Ltda.).…”

Section: Ecg Recordings and Analysissupporting

confidence: 60%

Cardioprotective effects of pharmacological blockade of the mitochondrial calcium uniporter on myocardial ischemia-reperfusion injury

et al. 2020

Self Cite

View full text Add to dashboard Cite

To evaluate whether the attenuation of mitochondrial Ca 2+ overload produced by pharmacological blockade of mitochondrial Ca 2+ uniporter (MCU) protects the myocardium against injuries caused by cardiac ischemia and reperfusion (CIR). Methods: CIR was induced in adult male Wistar rats (300-350 g) by occlusion of the left anterior descendent coronary artery (10 min), followed by reperfusion (120 min). Rats were treated with different doses of MCU blocker ruthenium red (RuR), administered 5 min before ischemia or reperfusion. Results: In untreated rats, the incidences of ventricular arrhythmias (VA), atrioventricular block (AVB) and the lethality (LET) induced by CIR were 85%, 79% and 70%, respectively. In rats treated with RuR before ischemia, the incidences of VA, AVB and LET were significantly reduced to 62%, 25% and 25%, respectively. In rats treated with RuR after ischemia, the incidences of VA, AVB and LET were significantly reduced to 50%, 25% and 25%, respectively. Conclusion: The significant reduction of the incidence of CIR-induced VA, AVB and LET produced by the treatment with RuR indicates that the attenuation of mitochondrial Ca 2+ overload produced by pharmacological blockade of MCU can protect the myocardium against injuries caused by CIR.

show abstract

Section: Ecg Recordings and Analysissupporting

confidence: 60%

Cardioprotective effects of pharmacological blockade of the mitochondrial calcium uniporter on myocardial ischemia-reperfusion injury

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The method for induction of CIR has previously been described by our lab 11 , 13 - 15 . Rats were anesthetized with urethane (1.25 g/kg), and fixed in the supine position.…”

Section: Methodsmentioning

confidence: 99%

“…After of 10 min of cardiac ischemia, the tourniquet was removed to allow coronary recirculation for 75 min (cardiac reperfusion). The cardiac electrical activity in all groups studied was monitored by electrocardiogram (ECG) system using a method previously described by our lab 11 , 13 - 15 . ECG analysis was performed during 100 min of duration (stabilization for 15 min, cardiac ischemia for 10 min and cardiac reperfusion for 75 min).…”

Section: Methodsmentioning

confidence: 99%

Pharmacological modulation of b-adrenoceptors as a new cardioprotective strategy for therapy of myocardial dysfunction induced by ischemia and reperfusion

et al. 2019

Self Cite

View full text Add to dashboard Cite

Purpose: To evaluate the cardioprotective response of the pharmacological modulation of β-adrenergic receptors (β-AR) in animal model of cardiac ischemia and reperfusion (CIR), in spontaneously hypertensive (SHR) and normotensive (NWR) rats. Methods: CIR was induced by the occlusion of left anterior descendent coronary artery (10 min) and reperfusion (75 min). The SHR was treated with β-AR antagonist atenolol (AT, 10 mg/kg, IV) 5 min before CIR, and NWR were treated with β-AR agonist isoproterenol (ISO, 0.5 mg/kg, IV) 5 min before CIR. Results: The treatment with AT increased the incidence of VA, AVB and LET in SHR, suggesting that spontaneous cardioprotection in hypertensive animals was abolished by blockade of β-AR. In contrast, the treatment with ISO significantly reduced the incidence of ventricular arrhythmia, atrioventricular blockade and lethality in NWR (30%, 20% and 20%, respectively), suggesting that the activation of β-AR stimulate cardioprotection in normotensive animals. Serum CK-MB were higher in SHR/CIR and NWR/CIR compared to respective SHAM group (not altered by treatment with AT or ISO). Conclusion: The pharmacological modulation of β-AR could be a new cardioprotective strategy for the therapy of myocardial dysfunctions induced by CIR related to cardiac surgery and cardiovascular diseases.

show abstract

“…Reestablishing blood flow is considered to be the best therapeutic strategy and conventional remedy in myocardial infarction to prevent the myocardium from further damage, but nevertheless apoptosis of cardiomyocytes is inevitable in ischemia/reperfusion (I/R) injury [ 2 ]. The increasing prevalence of myocardial infarction means that researchers and pharmacologists have high interest in investigating new therapeutic agents that can reduce myocardial infarction (MI) and cardiac damage by I/R injury [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Novel Potentials of the DPP-4 Inhibitor Sitagliptin against Ischemia-Reperfusion (I/R) Injury in Rat Ex-Vivo Heart Model

Al-awar

Almási

Szabó

et al. 2018

IJMS

View full text Add to dashboard Cite

Dipeptidyl peptidase-4 (DPP-4) inhibitors are a class of oral anti-diabetic drugs, implicated in pleiotropic secondary cardioprotective effects. The aim of the study was to unveil the unknown and possible cardioprotective targets that can be exerted by sitagliptin (Sitg) against ischemia-reperfusion (I/R) injury. Male wistar rats received 2 weeks’ Sitg oral treatment of different doses (25, 50, 100, and 150 mg/kg/day), or saline as a Control. Hearts were then isolated and subjected to two different I/R injury protocols: 10 min perfusion, 45 min regional ischemia, and 120 min reperfusion for infarct size (IS) measurement, or: 10 min perfusion, 45 min regional ischemia and 10 min reperfusion for biochemical analysis: nitric oxide synthases (NOSs) and DPP-4 activity, glucagon-like peptide-1 (GLP-1), Calcium, transient receptor potential vanilloid (TRPV)-1 and calcitonin gene-related peptide (CGRP) levels, transient receptor potential canonical (TRPC)-1 and e-NOS protein expression. NOS inhibitor (l-NAME) and TRPV-1 inhibitor (Capsazepine) were utilized to confirm the implication of both signaling mechanisms in DPP-4 inhibition-induced at the level of IS. Findings show that Sitg (50 mg) resulted in significant decrease in IS and DPP-4 activity, and significant increase in GLP-1, NOS activity, e-NOS expression, TRPV-1 level and TRPC-1 expression, compared to controls. Results of CGRP are in line with TRPV-1, as a downstream regulatory effect. NOS system and transient receptor potential (TRP) channels can contribute to DPP-4 inhibition-mediated cardioprotection against I/R injury using Sitagliptin.

show abstract

Cardioprotective effect of lipstatin derivative orlistat on normotensive rats submitted to cardiac ischemia and reperfusion

Cited by 11 publications

References 26 publications

Cardioprotective effects of pharmacological blockade of the mitochondrial calcium uniporter on myocardial ischemia-reperfusion injury

Cardioprotective effects of pharmacological blockade of the mitochondrial calcium uniporter on myocardial ischemia-reperfusion injury

Pharmacological modulation of b-adrenoceptors as a new cardioprotective strategy for therapy of myocardial dysfunction induced by ischemia and reperfusion

Novel Potentials of the DPP-4 Inhibitor Sitagliptin against Ischemia-Reperfusion (I/R) Injury in Rat Ex-Vivo Heart Model

Contact Info

Product

Resources

About