Comparison of platelet rich plasma versus fibrin glue on colonic anastomoses in rats

Dağlıoğlu, Yusuf Kenan; Düzgün, Özgül; Sarıcı, İnanç Şamil

doi:10.1590/s0102-865020180040000005

Cited by 13 publications

(25 citation statements)

References 24 publications

(26 reference statements)

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“…Many agents have been used to forestall adhesion formation by delimitating inflammatory reactions during peritoneal healing, thereby reducing the creation of a fibrinous network between serosal surfaces. [12][13][14]17,19,20] Although detecting the properties of KO that depress the formation of peritoneal adhesions may be an issue of another study, we think the administration of omega-3 sources (either KO or FO) decreases the inflammation on peritoneal surfaces in the critical stage of peritoneal wound healing.…”

Section: Discussionmentioning

confidence: 98%

“…[13] Leakage at the site of anastomosis has been reported to contribute to an increase in the length of hospital stays and medical costs. [14][15][16] Prevention of anastomosis leakage remains the most critical issue. Hence, highlighting the need for new treatment strategies to prevent anastomosis leakage is crucial.…”

Section: Discussionmentioning

confidence: 99%

“…Many investigators have researched on anastomotic wound healing process. [12,14,[17][18][19][20] And these studies also investigated the effectiveness of diverse systemic or local applied agents on the anastomotic healing process. [17,21,22] Inflammation during wound healing process stimulates the formation of fibrous bands between serosal surfaces ultimately.…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

Ferhatoğlu

Kıvılcım

Vural

et al. 2019

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: Oils from marine organisms have a different fatty acid composition. Fish oil (FO) has a high content of eicosapentaenoic and docosahexaenoic acids esterified to triacylglycerols; while in krill oil (KO), fatty acids are primarily esterified to phospholipids. This study aimed to compare the efficacy of two different, marine-derived omega-3 fatty acid sources in the wound healing of colon anastomoses rat model. METHODS: For the study, we used 42 male Wistar albino rats. The rats were divided into six groups with seven rats in each group-CO3: left colonic anastomosis (control group), sacrificed on the third day; KO3: left colonic anastomosis + oral KO, sacrificed on the third day; FO3: left colonic anastomosis + oral FO, sacrificed on the third day; CO7: left colonic anastomosis (control group), sacrificed on the seventh day; KO7: left colonic anastomosis + oral KO, sacrificed on the seventh day; FO7: left colonic anastomosis + oral FO, sacrificed on the seventh day. Peritoneal adhesions, anastomotic bursting pressures, hydroxyproline levels, and histological examination of the anastomotic tissue were evaluated. RESULTS: On day 7, bursting pressure and hydroxyproline measurements of the KO group was significantly higher than the FO group (p=0.012; p=0.002, respectively). Also, on day 7, a statistically significant difference was observed between the groups according to inflammatory cell infiltration, fibroblast activity, neoangiogenesis, and collagen deposition in favor of the KO group (p=0.023; p=0.028; p=0.016; p=0.012, respectively). CONCLUSION: Both KO and FO supplementation in patients before colorectal surgery may reduce some risk of anastomotic leakage; and KO might be a better alternative and excellent omega-3 source.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

Ferhatoğlu

Kıvılcım

Vural

et al. 2019

Ulus Travma Acil Cerrahi Derg

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“…This result shows that the HIPEC procedure significantly reduces ABP through its negative effects on anastomotic healing, which is in agreement with the findings of previous reports. [25][26][27] There was also a statistically significant difference between the 5-FU group and the 5-FU + PRP group (P = 0.047), which indicates that PRP significantly increases ABP. These findings suggest that the negative effect of HIPEC on anastomotic healing can be reversed by the application of PRP gel, which is supported by similar studies in the literature.…”

Section: Discussionmentioning

confidence: 99%

“…These findings suggest that the negative effect of HIPEC on anastomotic healing can be reversed by the application of PRP gel, which is supported by similar studies in the literature. 13,23,24,27 Since the 1970s, the positive effects of administration of autologous PRP gel obtained from the patients' own blood have been shown to improve healing in chronic wounds, such as diabetic foot ulcers and venous ulcers. Due to the advantage of preparing the treatment material from the patients' own blood, some researchers referred to PRP as 'key for self-regeneration'.…”

Section: Discussionmentioning

confidence: 99%

Effect of platelet‐rich plasma on colon anastomosis in rats in which hyperthermic intra‐peritoneal chemotherapy was performed using 5‐fluorouracil

Göksu

Alakuş

Ertan

et al. 2020

ANZ Journal of Surgery

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Background: Hyperthermic intra-peritoneal chemotherapy (HIPEC) performed in patients with peritoneal carcinomatosis has a cytotoxic effect on tumour cells. This cytotoxic effect also adversely affects the wound healing of anastomosis, which is frequently undertaken in this patient group, and causes anastomotic leakage and intestinal fistulas. Platelet-rich plasma (PRP) gel has been used in wound healing and has shown efficacy. We investigated the effect of PRP gel on colon anastomosis healing in rats treated with 5-fluorouracil (5-FU). Methods: Twenty-four rats were divided into three groups, each containing eight rats: group 1 (control) underwent colon anastomosis alone; group 2 (5-FU) underwent a colon anastomosis and HIPEC; and group 3 (5-FU + PRP) underwent a colon anastomosis and HIPEC and received topical PRP gel. The rats were sacrificed on the post-operative day 7 and the anastomotic bursting pressure (ABP) was measured. Tissue samples were obtained to measure the hydroxyproline level and investigate the histopathological changes on the anastomosis line. Results: ABP was higher in the control and 5-FU + PRP groups than in the 5-FU group (P = 0.009, P = 0.047). The tissue hydroxyproline levels were higher in the control and 5-FU + PRP groups than in the 5-FU group (P = 0.001, P = 0.030). The histopathological findings of the 5-FU + PRP group were better than those of the 5-FU group. Conclusion: This study showed that the application of PRP gel to colon anastomoses increased ABP in rats treated with HIPEC using 5-FU. It also showed positive effects on anastomotic healing by increasing the tissue hydroxyproline levels on the anastomosis line and reducing the inflammatory response.

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Fibrin sealants as an adequate treatment alternative to traditional suturing for confined bowel lesions: A hypothesis for future experimental research

et al. 2020

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Comparison of platelet rich plasma versus fibrin glue on colonic anastomoses in rats

Abstract: Fibrin glue and platelet rich plasma are shown to be effective in healing intestinal anastomoses without superior to each other.

Cited by 13 publications

References 24 publications

Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

Comparison of the effects of two different marine-derived omega- 3 fatty acid sources, krill oil and fish oil, on the healing of primary colonic anastomoses after colectomy applied Wistar albino rat model

Effect of platelet‐rich plasma on colon anastomosis in rats in which hyperthermic intra‐peritoneal chemotherapy was performed using 5‐fluorouracil

Fibrin sealants as an adequate treatment alternative to traditional suturing for confined bowel lesions: A hypothesis for future experimental research

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